GlycoEnzDB

General Information

Gene Symbol:

A4GNT

Protein name:

Alpha-1,4-N-acetylglucosaminyltransferase

Enzyme Class:

Glycosyltransferase

CAZy Family:

GT32

E.C. No:

2.4.1.*

Reaction (SNFG):

+

→

+

UDP

Reaction (IUBMB):

No specified reaction.

About

* Transfers GlcNAc(α1-4) to βGal preferentially present in O-glycans forming GlcNAc(α1-4)Galβ-R or terminal αGlcNAc.
* The enzyme shows preference to act on core-2 glycan rather than core-1 glycans indicating a role for GlcNAc(β1-6) in promoting its activity. It extends the core-1 arm of O-glycans.
* It can act on both arms of core-2 glycan to corm structures like GlcNAc(α1-4)Gal(β1-4)GlcNAc(β1-6)[GlcNAc(α1-4)Gal(β1-3)]GalNAc.
* A4GNT is expressed in stomach, gall bladder, duodenum, and thus such structures are normally restricted to gland & gastric mucins, particulalry MUC6 with a small amount also being associated with MUC5AC.
* αGlcNAc is also expressed in pancreatic ducts showing gastric metaplasia or pancreatic intraepithelial neoplasia 1 (PanIN-1).
* A4gnt knockout mice revealed that αGlcNAc serves as a tumor suppressor of differentiated type of gastric adenocarcinoma. In addition, αGlcNAc protects gastric gland mucous cells from H. pylori infection. Thus, αGlcNAc plays a dual role to prevent the gastric mucosa from gastric tumorigenesis.

Related Pathway

KEGG ORTHOLOGY:

K08251

KEGG Pathway:

No Record

Reactome:

R-HSA-913709

Related Database

Organism:

Homo sapiens

Uniprot:

Q9UNA3

NCBI GeneID:

51146

GlyMap GeneID:

51146

DNA_RefSeq:

NM_016161.2

Protein_RefSeq:

NP_057245.1

BRENDA:

Kinetic info.

OMIM number:

-

GeneCards database:

A4GNT

Enzyme Specificity

Glycan Type:

O_linked (mucin type)

Compartment:

Substrate:

Gal(b1-?)

Product:

GlcNAc(a1-4)Gal(b1-?)

Simulation Parameters

Mechaelis-Menten parameters (suggested)
Vm: (pmol/cell • hr) Km: (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)

ER	Cis-	Medial-	Trans-	TGN

Expression Data {ref. 5} Source from NCBI gene database

Expression data are collected from NCBI gene database

Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database

Transcription factor {ref. 10}

miRNA-GlycoEnzyme Interactome {ref. 9}

miRNA Name	Pos.	Context Score	Frac.	Wt. Context	Frac.	Conserved
hsa-let-7e-3p	302-309	-0.723	99.0	-0.723	99.0	noncon
hsa-miR-3136-5p	524-531	-0.607	99.0	-0.607	99.0	noncon
hsa-miR-22-3p	397-404	-0.601	99.0	-0.601	99.0	con
hsa-miR-6772-5p	182-189	-0.569	99.0	-0.569	99.0	noncon
hsa-miR-6769a-5p	216-223	-0.56	99.0	-0.56	99.0	noncon
hsa-miR-4777-3p	470-477	-0.549	99.0	-0.549	99.0	noncon
hsa-miR-575	167-174	-0.54	99.0	-0.54	99.0	noncon
hsa-miR-891a-3p	52-59	-0.534	99.0	-0.534	99.0	noncon
hsa-miR-6769b-5p	216-223	-0.534	99.0	-0.534	99.0	noncon
hsa-miR-660-5p	381-388	-0.53	99.0	-0.53	99.0	noncon
hsa-miR-4439	524-531	-0.526	99.0	-0.526	99.0	noncon
hsa-miR-4676-5p	167-174	-0.516	99.0	-0.516	99.0	noncon
hsa-miR-148b-3p	325-332	-0.498	99.0	-0.498	99.0	noncon
hsa-miR-152-3p	325-332	-0.498	99.0	-0.498	99.0	noncon
hsa-miR-148a-3p	325-332	-0.498	99.0	-0.498	99.0	noncon
hsa-miR-4683	69-76	-0.464	99.0	-0.464	99.0	noncon
hsa-miR-6513-5p	155-162	-0.461	99.0	-0.461	99.0	noncon
hsa-miR-3619-3p	189-196	-0.451	99.0	-0.451	99.0	noncon
hsa-miR-6881-3p	254-261	-0.439	98.0	-0.439	99.0	noncon
hsa-miR-1301-3p	342-349	-0.415	98.0	-0.415	99.0	noncon
hsa-miR-5047	342-349	-0.415	98.0	-0.415	98.0	noncon
hsa-miR-567	178-185	-0.412	99.0	-0.412	99.0	noncon
hsa-miR-4264	331-338	-0.412	99.0	-0.412	99.0	noncon
hsa-miR-5089-5p	154-161	-0.401	99.0	-0.401	99.0	noncon
hsa-miR-873-5p.2	290-297	-0.381	99.0	-0.381	99.0	noncon
hsa-miR-7977	355-362	-0.361	99.0	-0.361	99.0	noncon
hsa-miR-6875-3p	372-379	-0.358	98.0	-0.358	99.0	noncon
hsa-miR-4455	183-189	-0.332	91.0	-0.332	94.0	noncon
hsa-miR-6882-3p	396-402	-0.322	96.0	-0.322	97.0	noncon
hsa-miR-4274	200-206	-0.318	97.0	-0.318	98.0	noncon
hsa-miR-4712-5p	386-392	-0.306	96.0	-0.306	97.0	noncon
hsa-miR-5591-3p	382-388	-0.302	96.0	-0.302	97.0	noncon
hsa-miR-500a-3p	262-268	-0.3	96.0	-0.3	97.0	noncon
hsa-miR-6503-3p	493-499	-0.296	96.0	-0.296	97.0	noncon
hsa-miR-770-5p	386-392	-0.295	96.0	-0.295	97.0	noncon
hsa-miR-4264	467-473	-0.292	97.0	-0.292	97.0	noncon
hsa-miR-6855-3p	524-530	-0.29	96.0	-0.29	97.0	noncon
hsa-miR-1324	138-144	-0.29	97.0	-0.29	98.0	noncon
hsa-miR-4776-5p	190-196	-0.289	95.0	-0.289	96.0	noncon
hsa-miR-3184-3p	481-487	-0.282	97.0	-0.282	98.0	noncon
hsa-miR-6792-3p	369-375	-0.279	95.0	-0.279	96.0	noncon
hsa-miR-4258	85-91	-0.275	91.0	-0.275	93.0	noncon
hsa-miR-6780a-3p	255-261	-0.273	96.0	-0.273	97.0	noncon
hsa-miR-200b-5p	435-441	-0.269	97.0	-0.269	98.0	noncon
hsa-miR-200a-5p	435-441	-0.269	97.0	-0.269	98.0	noncon
hsa-miR-4697-3p	327-333	-0.267	92.0	-0.267	94.0	noncon
hsa-miR-4513	524-530	-0.266	95.0	-0.266	96.0	noncon
hsa-miR-3121-3p	422-429	-0.265	99.0	-0.265	99.0	noncon
hsa-miR-6804-3p	262-268	-0.263	92.0	-0.263	95.0	noncon
hsa-miR-609	183-189	-0.261	92.0	-0.261	94.0	noncon
hsa-miR-627-3p	250-257	-0.26	97.0	-0.26	98.0	noncon
hsa-miR-7153-3p	187-193	-0.258	96.0	-0.258	97.0	noncon
hsa-miR-450a-1-3p	156-162	-0.257	96.0	-0.257	97.0	noncon