GlycoEnzDB
The Comprehensive Glyco-enzyme Database
GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
ALG14


 General Info

 About

 Related Pathway

 Related Database

 Enzyme Specificity

 Simulation Para.

 Expression Data

 Cancer Cell Line

 Transcription factor

 miRNA-GlycoEnzyme


General Information
Gene Symbol:   
ALG14
Protein name:   
UDP-N-acetylglucosamine transferase subunit ALG14 homolog
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT1
E.C. No:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

* Alg13/Alg14 are part of a heterodimer that transfers second GlcNAc from UDP-GlcNAc to generate Dol-PP-GlcNAc2
* Cells lacking these activities grow slowly, and mutations in Alg13 leads to CDG-1r

Related Pathway
KEGG ORTHOLOGY:   
K07441
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q96F25 
NCBI GeneID:   
199857
GlyMap GeneID:   
199857
DNA_RefSeq:   
NM_001305242.1 
Protein_RefSeq:   
NP_001292171.1 
BRENDA:   
Kinetic info.
OMIM number:   
612866
GeneCards database:   
ALG14
Enzyme Specificity
Glycan Type:  
     N_linked
Compartment:  
     ER
Substrate:  
     GlcNAc(a1-?)Dol-PP
Product:  
     GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP
Simulation Parameters
Mechaelis-Menten parameters (suggested)
Vm:       (pmol/cell • hr)       Km:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression data are collected from NCBI gene database
Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
ALG14/hsa-miR-6508-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...AAACCAAAUUUCU UUUCUAGA ...
3'   CCACCCAGUACGU AAAGAUC U
 8mer 235-242 -0.477 99.0 -0.453 99.0 noncon
5'...AAGGUAGUUGGUG UUCUAGA U...
3'   CCACCCAGUACGUA AAGAUC U
 7mer-A1 4311-4317 -0.022 40.0 0.0 19.0 noncon
5'...GCUUGAGACUGUG UUUCUAG U...
3'   CCACCCAGUACGU AAAGAUC U
 7mer-m8 1252-1258 -0.02 27.0 -0.001 22.0 noncon
5'...UAAGCCUUGACUG UUCUAGA A...
3'   CCACCCAGUACGUA AAGAUC U
 7mer-A1 1900-1906 -0.01 17.0 0.0 17.0 noncon