GlycoEnzDB
The Comprehensive Glyco-enzyme Database
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B3GALT5


 General Info

 About

 Related Pathway

 Related Database

 Enzyme Specificity

 Simulation Para.

 Expression Data

 Cancer Cell Line

 Transcription factor

 miRNA-GlycoEnzyme


General Information
Gene Symbol:   
B3GALT5
Protein name:   
Beta-1,3-galactosyltransferase 5
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.*
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

* Produces type 1 chain linkage in glycolipids and core-3 O-glycans, but not N-linked glycoproteins.
* May be an important enzyme forming CA19-9 in colon cancer. Beyond serving as a biomarker, CA19-9 also promotes pancreatitis and pancreatic cancer (in mice).
* B3GalT5 efficiently transfers Gal to the GlcNAc of the glycolipid Lc3Cer and to the terminal GalNAc residue of the globoside Gb4.
* It may thus contribute to the formation of CA 19-9, SSEA-3 and elongated core-3 O-glycans.
* Expression is restricted to the colorectum, small intestine, stomach, and pancreas.

Related Pathway
KEGG ORTHOLOGY:   
K03877
KEGG Pathway:   
ko00601  ko00603  ko01100 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y2C3 
NCBI GeneID:   
10317
GlyMap GeneID:   
10317
DNA_RefSeq:   
NM_001278650.1 
Protein_RefSeq:   
NP_001265579.1 
BRENDA:   
Kinetic info.
OMIM number:   
604066
GeneCards database:   
B3GALT5
Enzyme Specificity
Glycan Type:  
     O_linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-?) or GalNAc(b1-?)
Product:  
     Gal(b1-3)GlcNAc(b1-?) or Gal(b1-3)GalNAc(b1-?)
Constraints:  
     !Gal(b1-4)@GlcNAc(b1-?)&!GalNAc(b1-4)@GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
Vm:       (pmol/cell • hr)       Km:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression data are collected from NCBI gene database
Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 10 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
B3GALT5/hsa-miR-323a-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...ACCUGAGACUCCAA GACCACCA ...
3'   CGCUUGCGCGGUGC CUGGUGG A
 8mer 6834-6841 -0.274 94.0 -0.274 95.0 noncon
5'...UGCCCCUCCACUGC GACCACC C...
3'   CGCUUGCGCGGUGC CUGGUGG A
 7mer-m8 3788-3794 -0.119 77.0 -0.119 84.0 noncon
5'...GAUUAUAGGCAUGC ACCACCA A...
3'   CGCUUGCGCGGUGCC UGGUGG A
 7mer-A1 4953-4959 -0.032 43.0 -0.032 58.0 noncon