GlycoEnzDB

General Information

Gene Symbol:

B3GALT5

Protein name:

Beta-1,3-galactosyltransferase 5

Enzyme Class:

Glycosyltransferase

CAZy Family:

GT31

E.C. No:

2.4.1.*

Reaction (SNFG):

	+		→		+	UDP
	+		→		+	UDP

Reaction (IUBMB):

No specified reaction.

About

* Produces type 1 chain linkage in glycolipids and core-3 O-glycans, but not N-linked glycoproteins.
* May be an important enzyme forming CA19-9 in colon cancer. Beyond serving as a biomarker, CA19-9 also promotes pancreatitis and pancreatic cancer (in mice).
* B3GalT5 efficiently transfers Gal to the GlcNAc of the glycolipid Lc3Cer and to the terminal GalNAc residue of the globoside Gb4.
* It may thus contribute to the formation of CA 19-9, SSEA-3 and elongated core-3 O-glycans.
* Expression is restricted to the colorectum, small intestine, stomach, and pancreas.

Related Pathway

KEGG ORTHOLOGY:

K03877

KEGG Pathway:

ko00601 ko00603 ko01100

Reactome:

R-HSA-9037629

Related Database

Organism:

Homo sapiens

Uniprot:

Q9Y2C3

NCBI GeneID:

10317

GlyMap GeneID:

10317

DNA_RefSeq:

NM_001278650.1

Protein_RefSeq:

NP_001265579.1

BRENDA:

Kinetic info.

OMIM number:

604066

GeneCards database:

B3GALT5

Enzyme Specificity

Glycan Type:

O_linked (mucin type), GSL

Compartment:

Cis, Medial, Trans, TGN

Substrate:

GlcNAc(b1-?) or GalNAc(b1-?)

Product:

Gal(b1-3)GlcNAc(b1-?) or Gal(b1-3)GalNAc(b1-?)

Constraints:

!Gal(b1-4)@GlcNAc(b1-?)&!GalNAc(b1-4)@GlcNAc(b1-?)

Simulation Parameters

Mechaelis-Menten parameters (suggested)
Vm: (pmol/cell • hr) Km: (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)

ER	Cis-	Medial-	Trans-	TGN

Expression Data {ref. 5} Source from NCBI gene database

Expression data are collected from NCBI gene database

Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database

Transcription factor {ref. 10}

No high degree of confidence data

miRNA-GlycoEnzyme Interactome {ref. 9}

B3GALT5/hsa-miR-4498

miRNA Target	Site Type	Pos.	Context Score	Frac.	Wt. Context	Frac.	Conserved
5'...UAACUGCCCCACAU CCAGCCCA ... 3' GUCGUGAACGGGAC GGUCGGG U	8mer	3613-3620	-0.292	92.0	-0.292	94.0	noncon
5'...AUAAACUCUUCAGU CCAGCCCA ... 3' GUCGUGAACGGGAC GGUCGGG U	8mer	8651-8658	-0.279	91.0	-0.279	93.0	noncon
5'...AUACACGCCUACAC CCAGCCC C... 3' GUCGUGAACGGGAC GGUCGGG U	7mer-m8	2816-2822	-0.161	73.0	-0.161	80.0	noncon
5'...CAAGUGUCCAAUGG CAGCCCA A... 3' GUCGUGAACGGGACG GUCGGG U	7mer-A1	8608-8614	-0.154	71.0	-0.154	79.0	noncon
5'...CUGUCCUGCCCUGG CCAGCCC U... 3' GUCGUGAACGGGAC GGUCGGG U	7mer-m8	8572-8578	-0.14	68.0	-0.14	76.0	noncon
5'...ACAGCAUCAGCUCA CAGCCCA C... 3' GUCGUGAACGGGACG GUCGGG U	7mer-A1	1854-1860	-0.137	67.0	-0.137	76.0	noncon
5'...UAGGAAGCUUGGGA CCAGCCC U... 3' GUCGUGAACGGGAC GGUCGGG U	7mer-m8	8377-8383	-0.114	62.0	-0.114	71.0	noncon
5'...UGUGCGCCUGUCCU CCAGCCC G... 3' GUCGUGAACGGGAC GGUCGGG U	7mer-m8	4758-4764	-0.091	55.0	-0.091	67.0	noncon
5'...UCCGCCUCCGGGUU CCAGCCC U... 3' GUCGUGAACGGGAC GGUCGGG U	7mer-m8	6369-6375	-0.043	39.0	-0.043	53.0	noncon