GlycoEnzDB
The Comprehensive Glyco-enzyme Database
GlycoEnzDB
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B4GALT2


 General Info

 About

 Related Pathway

 Related Database

 Enzyme Specificity

 Simulation Para.

 Expression Data

 Cancer Cell Line

 Transcription factor

 miRNA-GlycoEnzyme


General Information
Gene Symbol:   
B4GALT2
Protein name:   
Beta-1,4-galactosyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT7
E.C. No:   
2.4.1.*
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

* In enzymology assays, β4GalT1 and β4Gal2 display similar specificity for Type II LacNAc chains. Both enzymes preferring the branch of GlcNAcβ1-2Manα1-3 and GlcNAcβ1-4Manα1-3 arm over GlcNAcβ1-2Manα1-6. But, both can transfer β4-Gal residues to N-glycans in the tetra antennary glycan.

Related Pathway
KEGG ORTHOLOGY:   
K07967
KEGG Pathway:   
ko00052  ko00510  ko00513  ko00514  ko00515  ko00533  ko00601  ko01100 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
O60909 
NCBI GeneID:   
8704
GlyMap GeneID:   
8704
DNA_RefSeq:   
NM_001005417.2 
Protein_RefSeq:   
NP_001005417.1 
BRENDA:   
Kinetic info.
OMIM number:   
604013
GeneCards database:   
B4GALT2
Enzyme Specificity
Glycan Type:  
     N_linked, O_linked (mucin type), O_linked (not mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-?)
Product:  
     Gal(b1-4)GlcNAc(b1-?)
Constraints:  
     !Gal(b1-3)GlcNAc(b1-?)&!@GlcNAc(b1-4)...Man(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
Vm:   0.000575    (pmol/cell • hr)       Km:   150000000    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression data are collected from NCBI gene database
Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 180 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
B4GALT2/hsa-miR-185-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...CCCUCCCCCUAGCC CAGCCCCA ...
3'   CUGGUCUCCUUUCG GUCGGGG A
 8mer 251-258 -0.348 93.0 -0.348 94.0 noncon
5'...CCCCUGCUGAGGAG CAGCCCCA ...
3'   CUGGUCUCCUUUCG GUCGGGG A
 8mer 514-521 -0.353 93.0 -0.353 95.0 noncon
5'...AGAGAGGAGGCAGG AGCCCCA G...
3'   CUGGUCUCCUUUCGG UCGGGG A
 7mer-A1 647-653 -0.109 49.0 -0.109 63.0 noncon
5'...UGUCAGGGUCGGGC CAGCCCC U...
3'   CUGGUCUCCUUUCG GUCGGGG A
 7mer-m8 278-284 -0.076 40.0 -0.076 55.0 noncon