GlycoEnzDB
The Comprehensive Glyco-enzyme Database
GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
DDOST


 General Info

 About

 Related Pathway

 Related Database

 Enzyme Specificity

 Simulation Para.

 Expression Data

 Cancer Cell Line

 Transcription factor

 miRNA-GlycoEnzyme


General Information
Gene Symbol:   
DDOST
Protein name:   
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Enzyme Class:   
CAZy Family:   
E.C. No:   
Reaction (SNFG):   

No reaction →
Reaction (IUBMB):   
No specified reaction.
About

* A multi-membrane protein ER membrane associated protein complex that mediates the en-bloc transfer of 14 sugar LLO to N-X-S/T motif in nascent polypeptide
* STT3 is the catalytic core of this complex
* Stt3 forms a complex with the accessory membrane proteins DDOST/OST48, RPN1/ribophorin I, RPN2/ribophorin II, DAD1, Ost4, TUSC3/N33 and MGAT1/IAP in mammals for maximal ativity.
* There are two STT3 genes: STT3A and STT3B. Human Stt3A and Stt3B have redundant functions in the co-translational N-glycosylation. Stt3B also mediates the post-translational N-glycosylation after some time, rather than just at the time when the protein enters the ER from the ribosome.
* Glc[0-3]Man[0-9]GlcNAc2β1-PP-Dol + Asn-Xaa-Ser/Thr -> Dol-PP + Glc[0-3]Man[0-9]GlcNAc2β1-Asn-Xaa-Ser/Thr.

Related Pathway
KEGG ORTHOLOGY:   
K12670
KEGG Pathway:   
ko00510  ko00513  ko01100  ko04141 
Reactome:   
R-HSA-1799339
Related Database
Organism:   
Homo sapiens
Uniprot:   
P39656 
NCBI GeneID:   
1650
GlyMap GeneID:   
1650
DNA_RefSeq:   
NM_005216.4 
Protein_RefSeq:   
NP_005207.2 
BRENDA:   
Kinetic info.
OMIM number:   
602202
GeneCards database:   
DDOST
Enzyme Specificity
Glycan Type:  
     N_linked
Compartment:  
     ER
Substrate:  
     No reaction(-CHAR)
Product:  
     (-CHAR)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
Vm:       (pmol/cell • hr)       Km:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 140 120 100 80 60 40 20 0 RPKM
Expression data are collected from NCBI gene database
Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 600 500 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
DDOST/hsa-miR-520g-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'... AAAAGAAGUUUUUC CACUUUGA ...
3'   UGUGAGAUUUCCCUUC GUGAAAC A
 8mer 185-192 -0.453 99.0 -0.453 99.0 noncon
5'... GCCUGUAAUCCCAG CACUUUG G...
3'   UGUGAGAUUUCCCUUC GUGAAAC A
 7mer-m8 289-295 -0.02 44.0 -0.001 42.0 noncon