GlycoEnzDB
The Comprehensive Glyco-enzyme Database
GlycoEnzDB
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MGAT5B


 General Info

 About

 Related Pathway

 Related Database

 Enzyme Specificity

 Simulation Para.

 Expression Data

 Cancer Cell Line

 Transcription factor

 miRNA-GlycoEnzyme


General Information
Gene Symbol:   
MGAT5B
Protein name:   
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT18
E.C. No:   
2.4.1.*
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

* ~30 % of all O-linked glycans in the brain are O-mannose based. This is initiated by protein O-mannosyltransferase 1 (POMT1) and POMT 2, followed by POMGnT1 activity.
* A brain-specific β1,6-N-GlcNAcT (MGAT5B) has been cloned that transfers β1,6GlcNAc to a-mannose in GlcNAc-β1,2-Man-a sequence on both N-glycans and O-mannosyl glycans. In vivo this enzyme is dominantly considered to act on o-mannose glycans of the brain.
* Due to its activity, a variety of extended structures can be formed on the O-mannose backbone including sialylated glycans, glycans with HNK-1 epitope, and those with Lewis X structures.

Related Pathway
KEGG ORTHOLOGY:   
K09661
KEGG Pathway:   
ko00510  ko00515  ko01100 
Reactome:   
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q3V5L5 
NCBI GeneID:   
146664
GlyMap GeneID:   
146664
DNA_RefSeq:   
NM_001199172.1 
Protein_RefSeq:   
NP_001186101.1 
BRENDA:   
Kinetic info.
OMIM number:   
612441
GeneCards database:   
MGAT5B
Enzyme Specificity
Glycan Type:  
     O_linked (not mucin type)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-2)Man(a1-?)Ser|Thr
Product:  
     GlcNAc(b1-2)[GlcNAc(b1-6)]Man(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
Vm:   0.00001    (pmol/cell • hr)       Km:   130000000    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression data are collected from NCBI gene database
Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000428789 ENST00000565043 ENST00000565675 ENST00000568598 POU5F1 MYCN GRHL2
miRNA-GlycoEnzyme Interactome {ref. 9}
MGAT5B/hsa-miR-296-5p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...CCAGGUGGCGAAA GGGGCCCA ...
3'   UGUCCUAACUCCC CCCCGGG A
 8mer 348-355 -0.345 93.0 -0.345 95.0 noncon
5'...AAGGGUCCAAGGA GGGGCCC U...
3'   UGUCCUAACUCCC CCCCGGG A
 7mer-m8 446-452 -0.176 76.0 -0.176 82.0 noncon
5'...AGCCAGGCACACA GGGCCCA U...
3'   UGUCCUAACUCCCC CCCGGG A
 7mer-A1 548-554 -0.122 65.0 -0.122 74.0 noncon
5'...CCCAGUCGUUCUU GGGCCCA G...
3'   UGUCCUAACUCCCC CCCGGG A
 7mer-A1 365-371 -0.085 56.0 -0.085 67.0 noncon