GlycoEnzDB
The Comprehensive Glyco-enzyme Database
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Man(a1-0)Ser|ThrR(-CHAR).jpg)
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[GlcNAc(b1-6)]Man(a1-0)Ser|ThrR(-CHAR).jpg)
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* ~30 % of all O-linked glycans in the brain are O-mannose based. This is initiated by protein O-mannosyltransferase 1 (POMT1) and POMT 2, followed by POMGnT1 activity.
* A brain-specific β1,6-N-GlcNAcT (MGAT5B) has been cloned that transfers β1,6GlcNAc to a-mannose in GlcNAc-β1,2-Man-a sequence on both N-glycans and O-mannosyl glycans. In vivo this enzyme is dominantly considered to act on o-mannose glycans of the brain.
* Due to its activity, a variety of extended structures can be formed on the O-mannose backbone including sialylated glycans, glycans with HNK-1 epitope, and those with Lewis X structures.
| ER | Cis- | Medial- | Trans- | TGN |
|---|---|---|---|---|
| MGAT5B/hsa-miR-3116 |
|---|
| miRNA Target | Site Type | Pos. | Context Score | Frac. | Wt. Context | Frac. | Conserved | Quality |
|---|---|---|---|---|---|---|---|---|
|
5'...AAUCCCGCUCCCUC
UCCAGGCA
... 3' UCAGGGAUGAUACA AGGUCCG U |
8mer | 1263-1270 | -0.282 | 97.0 | -0.282 | 98.0 | noncon | |
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5'...UGGGUGGUACCUGC
UCCAGGCA
... 3' UCAGGGAUGAUACA AGGUCCG U |
8mer | 492-499 | -0.164 | 90.0 | -0.164 | 93.0 | noncon | |
|
5'...UCCUGUGAGGGGAG
CCAGGCA
C... 3' UCAGGGAUGAUACAA GGUCCG U |
7mer-A1 | 537-543 | -0.158 | 89.0 | -0.158 | 93.0 | noncon | |
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5'...GCCCCUGGGACCUC
CCAGGCA
G... 3' UCAGGGAUGAUACAA GGUCCG U |
7mer-A1 | 201-207 | -0.073 | 70.0 | -0.073 | 80.0 | noncon |