GlycoEnzDB
The Comprehensive Glyco-enzyme Database
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Man(a1-0)Ser|ThrR(-CHAR).jpg)
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[GlcNAc(b1-6)]Man(a1-0)Ser|ThrR(-CHAR).jpg)
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* ~30 % of all O-linked glycans in the brain are O-mannose based. This is initiated by protein O-mannosyltransferase 1 (POMT1) and POMT 2, followed by POMGnT1 activity.
* A brain-specific β1,6-N-GlcNAcT (MGAT5B) has been cloned that transfers β1,6GlcNAc to a-mannose in GlcNAc-β1,2-Man-a sequence on both N-glycans and O-mannosyl glycans. In vivo this enzyme is dominantly considered to act on o-mannose glycans of the brain.
* Due to its activity, a variety of extended structures can be formed on the O-mannose backbone including sialylated glycans, glycans with HNK-1 epitope, and those with Lewis X structures.
| ER | Cis- | Medial- | Trans- | TGN |
|---|---|---|---|---|
| MGAT5B/hsa-miR-6766-5p |
|---|
| miRNA Target | Site Type | Pos. | Context Score | Frac. | Wt. Context | Frac. | Conserved | Quality |
|---|---|---|---|---|---|---|---|---|
|
5'... UCAGAACACUGCCA
CCCACCCA
... 3' GAGUUAUUCUAGACGA GGGUGGG C |
8mer | 1234-1241 | -0.332 | 94.0 | -0.332 | 95.0 | noncon | |
|
5'... CCUCUCCAGGCAAC
CCCACCCA
... 3' GAGUUAUUCUAGACGA GGGUGGG C |
8mer | 1273-1280 | -0.185 | 74.0 | -0.185 | 81.0 | noncon | |
|
5'... GAUUUCCCGAUUCC
CCCACCC
U... 3' GAGUUAUUCUAGACGA GGGUGGG C |
7mer-m8 | 662-668 | -0.178 | 72.0 | -0.178 | 79.0 | noncon | |
|
5'... CCUUCAGAACACUG
CCACCCA
C... 3' GAGUUAUUCUAGACGAG GGUGGG C |
7mer-A1 | 1231-1237 | -0.126 | 59.0 | -0.126 | 70.0 | noncon | |
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5'... ACCCUAGGUAGCCC
CCCACCC
C... 3' GAGUUAUUCUAGACGA GGGUGGG C |
7mer-m8 | 1056-1062 | -0.079 | 47.0 | -0.079 | 61.0 | noncon | |
|
5'... UGGGGGUGUAGGGA
CCACCCA
G... 3' GAGUUAUUCUAGACGAG GGUGGG C |
7mer-A1 | 708-714 | -0.029 | 30.0 | -0.029 | 45.0 | noncon |