GlycoEnzDB
The Comprehensive Glyco-enzyme Database
GlycoEnzDB
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POMGNT1


 General Info

 About

 Related Pathway

 Related Database

 Enzyme Specificity

 Simulation Para.

 Expression Data

 Cancer Cell Line

 Transcription factor

 miRNA-GlycoEnzyme


General Information
Gene Symbol:   
POMGNT1
Protein name:   
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT13
E.C. No:   
2.4.1.*
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

* O-mannosyl glycans are a type of O-glycan in which the reducing terminal mannose is attached to Ser/Thr.
* POMGnT1 catalyzes the transfer of β1,2GlcNAc from UDP-GlcNAc to O-mannose of glycoproteins.
* POMGnT1 deficiency results in muscle-eye-brain disease (MEB), a form of congenital muscular dystrophy/ a-dystroglycanopathy.
* POMGnT1 cannot recognize 6P-Man-peptide as an acceptor. Thus O-mannose phosphorylation occurs after the formation of GlcNAcβ1-2Man linkage.

Related Pathway
KEGG ORTHOLOGY:   
K09666
KEGG Pathway:   
ko00515  ko01100 
Reactome:   
R-HSA-5083628
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8WZA1 
NCBI GeneID:   
55624
GlyMap GeneID:   
55624
DNA_RefSeq:   
NM_017739.3 
Protein_RefSeq:   
NP_060209.3 
BRENDA:   
Kinetic info.
OMIM number:   
606822
GeneCards database:   
POMGNT1
Enzyme Specificity
Glycan Type:  
     O_linked (not mucin type)
Compartment:  
     ER
Substrate:  
     Man(a1-?)Sre|Thr
Product:  
     GlcNAc(b1-2)Man(a1-?)Sre|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
Vm:       (pmol/cell • hr)       Km:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression data are collected from NCBI gene database
Cancer Cell Line Encyclopedia {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
POMGNT1/hsa-miR-660-3p
miRNA Target Site Type Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
5'...ACCCCACACCCAU CGAGUUCA ...
3'   AUUAGGUACGUGU GUCCUCC A
 8mer 291-298 -0.263 96.0 -0.263 97.0 noncon
5'...GCUAUGGAAGUGU AUGCAGC U...
3'   AUUAGGUACGUGUG UCCUCC A
 6mer 264-270 -0.157 86.0 -0.157 90.0 noncon
5'...AGUUGAGGGGUGA UGAAGUG A...
3'   AUUAGGUACGUGUG UCCUCC A
 6mer 910-916 -0.087 68.0 -0.087 79.0 noncon
5'...AUGAUGUCCCAUU CCCCUUC U...
3'   AUUAGGUACGUGUG UCCUCC A
 6mer 1615-1621 -0.088 68.0 -0.088 79.0 noncon
5'...UGGGGGGUGCCUC GGGUGGA G...
3'   AUUAGGUACGUGUG UCCUCC A
 7mer-A1 1045-1051 -0.08 65.0 -0.08 77.0 noncon
5'...AUGAGGUGAGCAG GACUUGG G...
3'   AUUAGGUACGUGUG UCCUCC A
 6mer 1028-1034 -0.02 28.0 -0.02 52.0 noncon