UDP-Gal [Cyto] | → | UDP-Gal[Golgi] |
* Multiple-segment transmembrane protein of the Golgi apparatus that delivers UDP-Gal, synthesized in the cytosol, into the Golgi lumen. This is an antiport that simultaneously exchanges luminal UMP
* This is specific for UDP-Gal and not UDP-GlcNAc. It can also transport UDP-GalNAc
* CHO-derived Lec8 are incapable of transporting UDP-Gal but are competent in UDP-GlcNAc and CMP-Sia transport
* Mutant cells accumulate Glc-Cer instead of lactosylceramide, and contain truncated N-glycans terminated at GlcNAc
* Human UDP-Gal transporter occurs in two isoforms: hUGT1 and hUGT2
* The incorporation of galactose residues into the link tetra-saccharide of proteoglycans seems to be surprisingly resistant to the deficiency of UDP-Gal, as the amounts of chondroitin sulfate and heparin sulfate were not reduced significantly in UDP-Gal transporter-deficient cells derived from MDCK cells
ER | Cis- | Medial- | Trans- | TGN |
---|---|---|---|---|
SLC35A2/hsa-miR-6887-3p |
---|
miRNA Target | Site Type | Pos. | Context Score | Frac. | Wt. Context | Frac. | Conserved | Quality |
---|---|---|---|---|---|---|---|---|
5'...CACUGAGACUUCU
GGAGGGG
U... 3' GAUCCUCCUUUCA CCUCCCC U |
7mer-m8 | 145-151 | -0.284 | 90.0 | -0.284 | 92.0 | noncon | |
5'...GGGGGGACACAAG
GGAGGGG
G... 3' GAUCCUCCUUUCA CCUCCCC U |
7mer-m8 | 28-34 | -0.266 | 88.0 | -0.266 | 91.0 | noncon | |
5'...ACCCCCUUCCCCU
GGAGGGG
G... 3' GAUCCUCCUUUCA CCUCCCC U |
7mer-m8 | 211-217 | -0.038 | 43.0 | -0.038 | 57.0 | noncon |