EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GALE



General Information
Gene Symbol:   
GALE
Protein name:   
UDP-glucose 4-epimerase
Enzyme Class:   
CAZy Family:   
E.C. No:   
5.1.3.2
Organs:   
Reaction (SNFG):   

UDP-Gal or UDP-Glc or UDP-GalNAc or UDP-GlcNAc → UDP-Glc or UDP-Gal or UDP-GlcNAc or UDP-GalNAc
Reaction (IUBMB):   
No specified reaction.
About

• Epimerizes glucose to galactose on the UDP-glucose sugar nucleotide.
• GALE impairment leads to the human disorder known as galactosemia III which is associated with impaired growth and cognitive deficiencies.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-5609977
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q14376 
NCBI GeneID:   
2582
GlyMap GeneID:   
2582
DNA aRefSeq:   
NM_000403.3 
Protein RefSeq:   
NP_000394.2 
BRENDA:   
Kinetic info.
OMIM number:   
606953
GeneCards database:   
GALE
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     UDP-Gal or UDP-Glc or UDP-GalNAc or UDP-GlcNAc
Product:  
     UDP-Glc or UDP-Gal or UDP-GlcNAc or UDP-GalNAc
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 60 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 220 200 180 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1909-5p 278-285 -0.687 99.0 -0.687 99.0 noncon
hsa-miR-6501-3p 293-300 -0.563 99.0 -0.563 99.0 noncon
hsa-miR-4650-5p 399-406 -0.558 99.0 -0.027 63.0 noncon
hsa-miR-4313 241-248 -0.556 99.0 -0.556 99.0 noncon
hsa-miR-661 274-281 -0.539 99.0 -0.539 99.0 noncon
hsa-miR-4458 106-113 -0.523 98.0 -0.523 98.0 con
hsa-let-7d-5p 106-113 -0.515 98.0 -0.515 98.0 con
hsa-miR-4500 106-113 -0.505 98.0 -0.505 98.0 con
hsa-let-7c-5p 106-113 -0.497 98.0 -0.497 98.0 con
hsa-miR-98-5p 106-113 -0.497 98.0 -0.497 98.0 con
hsa-let-7i-5p 106-113 -0.497 98.0 -0.497 98.0 con
hsa-let-7e-5p 106-113 -0.497 98.0 -0.497 98.0 con
hsa-let-7a-5p 106-113 -0.497 98.0 -0.497 98.0 con
hsa-let-7f-5p 106-113 -0.497 98.0 -0.497 98.0 con
hsa-let-7g-5p 106-113 -0.497 98.0 -0.497 98.0 con
hsa-let-7b-5p 106-113 -0.497 98.0 -0.497 98.0 con
hsa-miR-654-5p 135-142 -0.495 99.0 -0.495 99.0 noncon
hsa-miR-541-3p 135-142 -0.495 99.0 -0.495 99.0 noncon
hsa-miR-6803-5p 224-231 -0.493 98.0 -0.493 99.0 noncon
hsa-miR-3190-3p 196-203 -0.487 99.0 -0.487 99.0 noncon
hsa-miR-6751-5p 224-231 -0.48 98.0 -0.48 99.0 noncon
hsa-miR-874-5p 84-91 -0.466 98.0 -0.466 98.0 noncon
hsa-miR-4677-3p 358-365 -0.459 99.0 -0.023 62.0 noncon
hsa-miR-589-5p 354-361 -0.442 99.0 -0.022 65.0 noncon
hsa-miR-4685-5p 71-78 -0.434 98.0 -0.434 98.0 noncon
hsa-miR-6878-3p 148-155 -0.423 98.0 -0.423 98.0 noncon
hsa-miR-193b-5p 113-120 -0.412 97.0 -0.412 97.0 noncon
hsa-miR-6837-5p 71-78 -0.407 98.0 -0.407 98.0 noncon
hsa-miR-6731-3p 384-391 -0.39 98.0 -0.02 64.0 noncon
hsa-miR-7152-3p 18-24 -0.389 98.0 -0.389 98.0 noncon
hsa-miR-7106-5p 270-277 -0.386 99.0 -0.386 99.0 noncon
hsa-miR-3978 306-313 -0.359 99.0 -0.359 99.0 noncon
hsa-miR-608 376-382 -0.358 92.0 -0.018 30.0 noncon
hsa-miR-4722-3p 56-63 -0.357 99.0 -0.357 99.0 noncon
hsa-miR-664b-5p 132-139 -0.348 94.0 -0.348 96.0 noncon
hsa-miR-324-3p 348-354 -0.347 97.0 -0.018 58.0 noncon
hsa-miR-615-5p 222-228 -0.343 95.0 -0.343 96.0 noncon
hsa-miR-4651 376-382 -0.343 91.0 -0.018 33.0 noncon
hsa-miR-6727-3p 56-63 -0.339 99.0 -0.339 99.0 noncon
hsa-miR-1913 348-354 -0.335 97.0 -0.017 60.0 noncon
hsa-miR-550a-5p 276-282 -0.334 95.0 -0.334 97.0 noncon
hsa-miR-550a-3-5p 276-282 -0.334 95.0 -0.334 97.0 noncon
hsa-miR-199b-5p 343-349 -0.326 95.0 -0.073 78.0 noncon
hsa-miR-199a-5p 343-349 -0.326 95.0 -0.073 78.0 noncon
hsa-miR-4680-5p 211-218 -0.32 97.0 -0.32 98.0 noncon
hsa-miR-7160-3p 69-75 -0.318 96.0 -0.318 97.0 noncon
hsa-miR-4731-5p 116-122 -0.313 97.0 -0.313 97.0 noncon
hsa-miR-6504-5p 394-400 -0.309 97.0 -0.016 52.0 noncon
hsa-miR-1271-3p 276-282 -0.301 94.0 -0.301 96.0 noncon
hsa-miR-1252-3p 282-289 -0.301 99.0 -0.301 99.0 noncon
hsa-miR-2113 257-264 -0.3 98.0 -0.3 98.0 noncon
hsa-miR-765 266-272 -0.297 94.0 -0.297 95.0 noncon
hsa-miR-6779-5p 271-277 -0.292 94.0 -0.292 96.0 noncon
hsa-miR-6775-3p 67-73 -0.292 92.0 -0.292 94.0 noncon
hsa-miR-4665-5p 225-231 -0.29 92.0 -0.29 94.0 noncon
hsa-miR-3064-5p 394-400 -0.287 96.0 -0.015 51.0 noncon
hsa-miR-1254 275-281 -0.285 97.0 -0.285 98.0 noncon
hsa-miR-711 204-210 -0.284 96.0 -0.284 97.0 noncon
hsa-miR-519e-3p 277-283 -0.282 94.0 -0.282 96.0 noncon
hsa-miR-30b-3p 271-277 -0.281 94.0 -0.281 96.0 noncon
hsa-miR-33b-3p 277-283 -0.278 94.0 -0.278 96.0 noncon
hsa-miR-7155-5p 115-121 -0.271 96.0 -0.271 97.0 noncon
hsa-miR-5699-5p 347-353 -0.27 96.0 -0.014 33.0 noncon
hsa-miR-3689b-3p 271-277 -0.27 93.0 -0.27 95.0 noncon
hsa-miR-3689c 271-277 -0.27 93.0 -0.27 95.0 noncon
hsa-miR-3918 70-76 -0.266 94.0 -0.266 95.0 noncon
hsa-miR-3165 374-380 -0.265 95.0 -0.014 40.0 noncon
hsa-miR-1972 400-406 -0.264 95.0 -0.014 37.0 noncon
hsa-miR-6745 375-381 -0.259 93.0 -0.014 29.0 noncon
hsa-miR-550b-2-5p 256-262 -0.259 94.0 -0.259 96.0 noncon
hsa-miR-1273h-5p 271-277 -0.259 91.0 -0.259 94.0 noncon
hsa-miR-4725-5p 202-208 -0.256 91.0 -0.256 94.0 noncon
hsa-miR-515-3p 277-283 -0.252 95.0 -0.252 97.0 noncon
hsa-miR-6780a-5p 271-277 -0.251 92.0 -0.251 94.0 noncon