EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GNE



General Information
Gene Symbol:   
GNE
Protein name:   
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Enzyme Class:   
CAZy Family:   
E.C. No:   
3.2.1.183
Organs:   
Reaction (SNFG):   

UDP-GlcNAc or ManNAc → ManNAc or ManNAc(6P)
Reaction (IUBMB):   
No specified reaction.
About

• Bifunctional enzyme responsible for epimerization of UDP-GlcNAc to UDP-ManNAc, and the phosphorylation of UDP-ManNAc to UDP-ManNAc 1 phosphate.
• Dysfunctional GNE results in two disorders in Human: sialuria and GNE-myopathy. Sialuria is caused by the decreased feedback inhibition of GNE via the accumulation of CMP-sialic acids. GNE-myopathy is a late onset disease where muscle weakness manifests later in life. The molecular mechanisms are unclear, but increased sialic acid is observed in patients.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y223 
NCBI GeneID:   
10020
GlyMap GeneID:   
10020
DNA aRefSeq:   
NM_001128227.2 
Protein RefSeq:   
NP_001121699.1 
BRENDA:   
Kinetic info.
OMIM number:   
603824
GeneCards database:   
GNE
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     UDP-GlcNAc or ManNAc
Product:  
     ManNAc or ManNAc(6P)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1271-5p 260-267 -0.471 98.0 -0.471 99.0 con
hsa-miR-96-5p 260-267 -0.458 98.0 -0.458 99.0 con
hsa-miR-24-3p 2879-2886 -0.452 99.0 -0.432 99.0 con
hsa-miR-6885-5p 2108-2115 -0.425 92.0 -0.406 93.0 noncon
hsa-miR-2276-5p 1092-1099 -0.419 99.0 -0.419 99.0 noncon
hsa-miR-6769b-3p 282-289 -0.392 98.0 -0.392 98.0 noncon
hsa-miR-675-5p 629-636 -0.385 93.0 -0.385 94.0 noncon
hsa-miR-4723-3p 282-289 -0.384 98.0 -0.384 98.0 noncon
hsa-miR-4278 1256-1263 -0.362 94.0 -0.362 96.0 noncon
hsa-miR-328-5p 2108-2115 -0.347 90.0 -0.332 92.0 noncon
hsa-miR-4433b-3p 168-175 -0.343 98.0 -0.343 98.0 noncon
hsa-miR-4723-5p 1237-1244 -0.34 90.0 -0.34 93.0 noncon
hsa-miR-6846-3p 213-220 -0.331 96.0 -0.331 97.0 noncon
hsa-miR-3183 282-289 -0.327 98.0 -0.327 99.0 noncon
hsa-miR-1178-5p 1958-1965 -0.315 96.0 -0.302 96.0 noncon
hsa-miR-134-5p 245-252 -0.314 96.0 -0.314 97.0 noncon
hsa-miR-1273f 669-676 -0.314 98.0 -0.314 98.0 noncon
hsa-miR-513a-5p 1192-1199 -0.311 98.0 -0.311 98.0 noncon
hsa-miR-3118 245-252 -0.306 95.0 -0.306 96.0 noncon
hsa-miR-610 223-230 -0.301 96.0 -0.301 97.0 noncon
hsa-miR-4466 629-636 -0.301 91.0 -0.301 93.0 noncon
hsa-miR-4476 2477-2484 -0.3 98.0 -0.287 98.0 noncon
hsa-miR-183-5p.2 260-266 -0.296 94.0 -0.296 96.0 con
hsa-miR-6876-5p 2477-2484 -0.292 98.0 -0.28 98.0 noncon
hsa-miR-3170 1051-1058 -0.291 95.0 -0.291 97.0 noncon
hsa-miR-651-5p 1711-1718 -0.285 97.0 -0.273 98.0 noncon
hsa-miR-5589-3p 2367-2374 -0.283 96.0 -0.271 97.0 noncon
hsa-miR-6862-3p 2623-2630 -0.272 94.0 -0.261 95.0 noncon
hsa-miR-7109-5p 2109-2116 -0.27 90.0 -0.259 92.0 noncon
hsa-miR-1271-3p 2162-2169 -0.264 91.0 -0.253 93.0 noncon
hsa-miR-6855-5p 1051-1058 -0.262 94.0 -0.262 95.0 noncon
hsa-miR-4297 2789-2795 -0.26 91.0 -0.249 93.0 noncon
hsa-miR-802 2870-2876 -0.252 96.0 -0.242 97.0 noncon
hsa-miR-6784-3p 2623-2630 -0.251 92.0 -0.241 94.0 noncon