EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
FUT8



General Information
Gene Symbol:   
FUT8
Protein name:   
Alpha-(1,6)-fucosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT23
E.C. No:   
2.4.1.68
Organs:   
Reaction (SNFG):   
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• Mediates the α1,6-fucosylation of N-glycans in many eukaryotes, but not plants and fungi.
• FUT8 primarily acts on complex and hybrid N-glycans as it requires GlcNAc-beta attached to Asn, and also the β1,2 GlcNAc residue linked to the α1,3 mannose arm.
• In some instances this may be cancer specific as core fucosylation is observed specifically on hepatocellular carcinoma (HCC), but not in chronic liver disease.
• Depletion of the core fucose in human IgG1 enhances the antibody-dependent cellular cytotoxicity (ADCC) activity.
• FUT8 activity is inhibited by bisecting GlcNAc (produced by MGAT3) and also terminal fucosylation.
• FUT8 is highly expressed in brain tissue, human blood platelet, cancer tissues and cancer cell lines.

Related Pathway
KEGG ORTHOLOGY:   
K00717
KEGG Pathway:   
ko00510  ko00513  ko00533  ko01100  ko05202 
Reactome:   
R-HSA-975578
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9BYC5 
NCBI GeneID:   
2530
GlyMap GeneID:   
2530
DNA aRefSeq:   
NM_004480.4 
Protein RefSeq:   
NP_004471.4 
BRENDA:   
Kinetic info.
OMIM number:   
602589
GeneCards database:   
FUT8
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     ...GlcNAc(b1-?)Asn
Product:  
     ...[Fuc(a1-6)]GlcNAc(b1-?)Asn
Constraints:  
     nGlcNAc(b1-2)Man(a1-3)[Man(a1-6)]Man(b1-4)GlcNAc(b1-4)GlcNAc(b1-?)>=1&!GlcNAc(b1-4)...Man(b1-4)&!Fuc(a1-3)&!Fuc(a1-3)...GlcNAc(b1-?)Asn
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.80e-05    (pmol/cell • hr)       KM:    2.50e+07    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-449b-5p 296-303 -0.578 99.0 -0.578 99.0 con
hsa-miR-34c-5p 296-303 -0.562 99.0 -0.562 99.0 con
hsa-miR-449a 296-303 -0.56 99.0 -0.56 99.0 con
hsa-miR-34a-5p 296-303 -0.544 99.0 -0.544 99.0 con
hsa-miR-7843-3p 156-163 -0.542 99.0 -0.542 99.0 noncon
hsa-miR-1256 653-660 -0.468 99.0 -0.468 99.0 noncon
hsa-miR-5192 627-634 -0.468 99.0 -0.468 99.0 noncon
hsa-miR-8065 1647-1654 -0.456 98.0 -0.252 92.0 noncon
hsa-miR-3135a 1514-1521 -0.411 97.0 -0.228 89.0 noncon
hsa-miR-628-5p 1657-1664 -0.41 98.0 -0.228 96.0 noncon
hsa-miR-4493 1539-1546 -0.406 98.0 -0.226 93.0 noncon
hsa-miR-4666b 239-246 -0.402 98.0 -0.402 99.0 noncon
hsa-miR-597-5p 319-326 -0.397 98.0 -0.397 98.0 noncon
hsa-miR-3616-3p 196-203 -0.386 97.0 -0.386 97.0 noncon
hsa-miR-6851-5p 1040-1047 -0.377 97.0 -0.211 89.0 noncon
hsa-miR-3689a-3p 1622-1629 -0.37 97.0 -0.207 92.0 noncon
hsa-miR-3689c 1622-1629 -0.37 97.0 -0.207 92.0 noncon
hsa-miR-30b-3p 1622-1629 -0.37 97.0 -0.207 92.0 noncon
hsa-miR-3689b-3p 1622-1629 -0.37 97.0 -0.207 92.0 noncon
hsa-miR-1273h-5p 1622-1629 -0.37 97.0 -0.207 91.0 noncon
hsa-miR-198 688-695 -0.366 98.0 -0.366 99.0 noncon
hsa-miR-342-3p 335-342 -0.363 99.0 -0.363 99.0 con
hsa-miR-6779-5p 1622-1629 -0.362 97.0 -0.203 91.0 noncon
hsa-miR-383-5p.2 88-95 -0.359 98.0 -0.359 99.0 noncon
hsa-miR-6837-3p 596-603 -0.359 98.0 -0.359 98.0 noncon
hsa-miR-6780a-5p 1622-1629 -0.357 97.0 -0.2 91.0 noncon
hsa-miR-7156-3p 188-195 -0.355 97.0 -0.355 98.0 noncon
hsa-miR-3171 604-611 -0.354 97.0 -0.354 98.0 noncon
hsa-miR-203b-3p 47-54 -0.351 99.0 -0.351 99.0 noncon
hsa-miR-3150b-3p 1380-1387 -0.343 98.0 -0.193 92.0 noncon
hsa-miR-4784 1380-1387 -0.343 98.0 -0.193 92.0 noncon
hsa-miR-1206 659-666 -0.335 98.0 -0.335 98.0 noncon
hsa-miR-6879-5p 1144-1151 -0.334 97.0 -0.188 90.0 noncon
hsa-miR-4688 265-271 -0.332 96.0 -0.332 97.0 noncon
hsa-miR-139-5p 69-76 -0.326 98.0 -0.326 98.0 noncon
hsa-miR-3184-3p 339-346 -0.325 98.0 -0.325 99.0 noncon
hsa-miR-4436b-3p 1144-1151 -0.318 97.0 -0.179 89.0 noncon
hsa-miR-4281 1262-1269 -0.314 99.0 -0.177 94.0 noncon
hsa-miR-8064 1231-1238 -0.313 97.0 -0.177 93.0 noncon
hsa-miR-4661-3p 1510-1517 -0.31 95.0 -0.175 89.0 noncon
hsa-miR-6132 1161-1167 -0.309 95.0 -0.175 87.0 noncon
hsa-miR-3689d 1040-1047 -0.307 97.0 -0.173 91.0 noncon
hsa-miR-4695-5p 1417-1424 -0.305 97.0 -0.172 91.0 noncon
hsa-miR-4268 809-815 -0.304 97.0 -0.283 97.0 noncon
hsa-miR-6735-5p 1144-1151 -0.302 96.0 -0.171 88.0 noncon
hsa-miR-2116-3p 1270-1277 -0.302 98.0 -0.171 96.0 noncon
hsa-miR-4693-3p 626-632 -0.301 95.0 -0.301 96.0 noncon
hsa-miR-6743-5p 265-271 -0.3 95.0 -0.3 96.0 noncon
hsa-miR-144-3p 1565-1572 -0.298 97.0 -0.169 94.0 noncon
hsa-miR-133a-3p.1 1263-1270 -0.298 91.0 -0.169 81.0 noncon
hsa-miR-630 601-608 -0.296 95.0 -0.296 96.0 noncon
hsa-miR-6810-5p 280-286 -0.295 96.0 -0.295 97.0 noncon
hsa-miR-887-5p 1623-1630 -0.29 96.0 -0.164 91.0 noncon
hsa-miR-6758-3p 480-487 -0.287 98.0 -0.287 98.0 noncon
hsa-miR-4687-5p 81-87 -0.286 97.0 -0.286 97.0 noncon
hsa-miR-4802-5p 1042-1048 -0.286 96.0 -0.162 89.0 noncon
hsa-miR-4762-3p 608-615 -0.284 98.0 -0.284 98.0 noncon
hsa-miR-6836-5p 1161-1167 -0.283 94.0 -0.16 86.0 noncon
hsa-miR-593-5p 352-358 -0.28 91.0 -0.28 94.0 noncon
hsa-miR-4330 362-369 -0.278 98.0 -0.278 98.0 noncon
hsa-miR-4423-3p 354-360 -0.277 93.0 -0.277 96.0 noncon
hsa-miR-4689 1381-1387 -0.27 96.0 -0.153 89.0 noncon
hsa-miR-583 175-182 -0.268 98.0 -0.268 98.0 noncon
hsa-miR-767-5p 351-357 -0.266 96.0 -0.266 97.0 noncon
hsa-miR-1267 468-475 -0.265 96.0 -0.265 97.0 noncon
hsa-miR-1226-5p 197-203 -0.264 95.0 -0.264 96.0 noncon
hsa-miR-218-1-3p 1133-1140 -0.262 93.0 -0.149 86.0 noncon
hsa-miR-7843-5p 1144-1151 -0.261 97.0 -0.148 91.0 noncon
hsa-miR-5187-3p 474-481 -0.259 97.0 -0.259 98.0 noncon
hsa-miR-1233-3p 82-88 -0.258 95.0 -0.258 96.0 noncon
hsa-miR-4632-5p 1144-1151 -0.253 97.0 -0.144 91.0 noncon
hsa-miR-4694-3p 1672-1679 -0.253 98.0 -0.144 94.0 noncon
hsa-miR-4786-3p 156-162 -0.252 94.0 -0.252 96.0 noncon
hsa-miR-5586-3p 625-631 -0.251 96.0 -0.251 97.0 noncon
hsa-miR-4653-5p 882-889 -0.251 96.0 -0.143 92.0 noncon
hsa-miR-5681a 268-275 -0.251 96.0 -0.251 97.0 noncon