EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GALT2



General Information
Gene Symbol:   
B3GALT2
Protein name:   
Beta-1,3-galactosyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.86
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Forms type 1 LacNAc chains (Galβ1-3GlcNAc) primarily in cell types derived from the endoderm (lung, thyroid, pancreas etc.).
• Helps form Lewisa and Lewisb antigens, and also sialyl-Lea (CA19-9) and disialyl-Lea (FH7).
• Three enzymes may form the type I LacNAc chain (B3GalT-1, -2 and -5). The expression pattern of these three enzymes vary with B3Gal-T1 being restricted to brain as well as intestine and colon; B3Gal-T2 in heart, brain, and bone marrow; and B3Gal-T5 in the gastrointestinal tract.
• B3Gal-T1 showed activity toward simple βGlcNAc saccharides and lactoseries glycosphingolipids, but not N-glycans on ovalbumin and asialo-agalacto-fetuin.
• B3Gal-T2 shows activity with both simple βGlcNAc saccharides, βGlcNAc terminating lactoseries glycosphingolipids, and ovalbumin and asialoagalacto-fetuin N-glycoprotein acceptors.
• B3Gal-T5 produces type 1 chain linkage in glycolipids and core-3 O-glycans, but not N-linked glycoproteins.
• Thus, multiple enzymes act on GSLs, but only B3GalT2 is the primary enzyme modifying N-glycans.

Related Pathway
KEGG ORTHOLOGY:   
K07820
KEGG Pathway:   
ko00601  ko01100 
Reactome:   
R-HSA-9037629
Related Database
Organism:   
Homo sapiens
Uniprot:   
O43825 
NCBI GeneID:   
8707
GlyMap GeneID:   
8707
DNA aRefSeq:   
NM_003783.3 
Protein RefSeq:   
NP_003774.1 
BRENDA:   
Kinetic info.
OMIM number:   
603018
GeneCards database:   
B3GALT2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-?)
Product:  
     Gal(b1-3)GlcNAc(b1-?)
Constraints:  
     !Gal(b1-4)GlcNAc(b1-?)&!@GlcNAc(b1-4)...Man(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000367434 EBF3 GATA1 GATA3 HOXC9 ELF3 ASCL1 STAT5A TEAD4 ATF2 ZNF629 HOXA2 PRDM10 ZNF652 FOXA1 RUNX1 USF2 ZBTB42 BATF TAL1 PKNOX1 KLF9 KMT2A TFAP4 TCF4 ASCL1 MYF5
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4491 314-321 -0.583 99.0 -0.583 99.0 noncon
hsa-miR-4657 314-321 -0.575 99.0 -0.575 99.0 noncon
hsa-miR-3679-3p 632-639 -0.548 99.0 -0.548 99.0 noncon
hsa-miR-550b-2-5p 889-896 -0.534 99.0 -0.534 99.0 noncon
hsa-miR-183-5p.2 1062-1069 -0.486 98.0 -0.486 99.0 con
hsa-miR-142-3p.2 1486-1493 -0.469 99.0 -0.001 30.0 noncon
hsa-miR-32-5p 23-29 -0.451 99.0 -0.451 99.0 con
hsa-miR-188-5p 1439-1446 -0.443 99.0 -0.001 26.0 noncon
hsa-miR-367-3p 23-29 -0.441 98.0 -0.441 99.0 con
hsa-miR-92a-3p 23-29 -0.44 99.0 -0.44 99.0 con
hsa-miR-4445-5p 1408-1415 -0.433 98.0 -0.001 22.0 noncon
hsa-miR-25-3p 23-29 -0.426 98.0 -0.426 99.0 con
hsa-miR-363-3p 23-29 -0.419 98.0 -0.419 99.0 con
hsa-miR-8082 1288-1295 -0.418 99.0 -0.001 23.0 noncon
hsa-miR-92b-3p 23-29 -0.407 98.0 -0.407 99.0 con
hsa-miR-943 341-348 -0.404 98.0 -0.404 99.0 noncon
hsa-miR-4666b 236-243 -0.399 98.0 -0.399 99.0 noncon
hsa-miR-105-3p 70-76 -0.398 95.0 -0.398 96.0 noncon
hsa-miR-4703-5p 37-44 -0.397 99.0 -0.397 99.0 noncon
hsa-miR-6804-3p 929-936 -0.392 97.0 -0.392 98.0 noncon
hsa-miR-3121-5p 443-450 -0.391 98.0 -0.391 99.0 noncon
hsa-miR-4766-3p 38-45 -0.387 98.0 -0.387 99.0 noncon
hsa-miR-3942-5p 37-44 -0.383 99.0 -0.383 99.0 noncon
hsa-miR-20a-5p 1204-1210 -0.364 99.0 -0.364 99.0 con
hsa-miR-3591-5p 1487-1494 -0.364 98.0 -0.001 27.0 noncon
hsa-miR-106b-5p 1204-1210 -0.364 99.0 -0.364 99.0 con
hsa-miR-6866-3p 1439-1446 -0.362 99.0 -0.001 34.0 noncon
hsa-miR-106a-5p 1204-1210 -0.354 98.0 -0.354 99.0 con
hsa-miR-4299 1449-1456 -0.353 99.0 -0.001 29.0 noncon
hsa-miR-93-5p 1204-1210 -0.35 99.0 -0.35 99.0 con
hsa-miR-20b-5p 1204-1210 -0.35 98.0 -0.35 99.0 con
hsa-miR-17-5p 1204-1210 -0.35 98.0 -0.35 99.0 con
hsa-miR-450a-1-3p 186-193 -0.346 98.0 -0.346 99.0 noncon
hsa-miR-519d-3p 1204-1210 -0.339 98.0 -0.339 99.0 con
hsa-miR-561-3p 1242-1249 -0.337 99.0 -0.337 99.0 noncon
hsa-miR-522-3p 817-824 -0.332 99.0 -0.332 99.0 noncon
hsa-miR-676-3p 77-84 -0.325 98.0 -0.325 98.0 noncon
hsa-miR-4309 1286-1292 -0.322 97.0 -0.001 47.0 noncon
hsa-miR-4534 1288-1295 -0.318 98.0 -0.001 34.0 noncon
hsa-miR-491-3p 253-260 -0.313 95.0 -0.313 97.0 noncon
hsa-miR-4277 60-67 -0.313 98.0 -0.313 99.0 noncon
hsa-miR-657 1077-1084 -0.309 97.0 -0.309 98.0 noncon
hsa-miR-224-3p 817-824 -0.308 99.0 -0.308 99.0 noncon
hsa-miR-6833-5p 505-512 -0.303 98.0 -0.303 98.0 noncon
hsa-miR-659-3p 753-760 -0.299 97.0 -0.299 98.0 noncon
hsa-miR-653-5p 712-719 -0.297 98.0 -0.297 99.0 con
hsa-miR-4539 524-531 -0.296 98.0 -0.296 98.0 noncon
hsa-miR-598-3p 651-657 -0.294 90.0 -0.294 94.0 noncon
hsa-miR-519b-3p 1203-1209 -0.292 97.0 -0.292 98.0 noncon
hsa-miR-519a-3p 1203-1209 -0.292 97.0 -0.292 98.0 noncon
hsa-miR-519c-3p 1203-1209 -0.292 97.0 -0.292 98.0 noncon
hsa-miR-526b-3p 1204-1210 -0.291 99.0 -0.291 99.0 con
hsa-miR-874-5p 284-290 -0.286 92.0 -0.286 94.0 noncon
hsa-miR-1205 1079-1085 -0.282 97.0 -0.282 98.0 noncon
hsa-miR-4709-5p 906-913 -0.277 97.0 -0.277 98.0 noncon
hsa-miR-4456 1450-1456 -0.274 94.0 -0.001 30.0 noncon
hsa-miR-3198 1286-1292 -0.274 97.0 -0.001 54.0 noncon
hsa-miR-4328 207-213 -0.272 98.0 -0.272 98.0 noncon
hsa-miR-183-3p 327-334 -0.268 99.0 -0.268 99.0 noncon
hsa-miR-4684-3p 977-983 -0.268 95.0 -0.268 96.0 noncon
hsa-miR-510-3p 713-720 -0.265 99.0 -0.265 99.0 noncon
hsa-miR-494-3p 711-718 -0.263 98.0 -0.263 99.0 con
hsa-miR-421 907-914 -0.262 96.0 -0.262 97.0 noncon
hsa-miR-7153-5p 546-553 -0.258 95.0 -0.258 97.0 noncon
hsa-miR-433-3p 1292-1299 -0.257 96.0 -0.001 33.0 noncon
hsa-miR-3614-5p 1552-1558 -0.257 96.0 -0.001 32.0 noncon
hsa-miR-23a-3p 663-670 -0.256 93.0 -0.256 95.0 con
hsa-miR-23c 663-670 -0.256 93.0 -0.256 95.0 con
hsa-miR-23b-3p 663-670 -0.256 93.0 -0.256 95.0 con
hsa-miR-130a-5p 663-670 -0.254 93.0 -0.254 95.0 con
hsa-miR-1271-5p 1063-1069 -0.254 93.0 -0.254 95.0 con