EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
UGT8



General Information
Gene Symbol:   
UGT8
Protein name:   
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT1
E.C. No:   
2.4.1.47
Organs:   
Reaction (SNFG):   
+ Cer →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Involved in galactosylation of ceramide in ER. Reaction product galactosylceramide (GalCer) is used in synthesis of GalCer series GSLs including sulfatide.
• GalCers play an important role in myelin, since GalCers and sulfatides comprise 23 % and 4 % of the total lipid content in the myelin sheath respectively. In mice lacking this enzyme activity, myelin is unstable and degenerates resulting in the formation of large vacuoles in the ventral white matter of the spinal cord and the brain.
• CGT/UGT8-deficient mice exhibit a generalized tremor at 12-14 days followed by hind-limb paralysis, and death by 30-60 days.
• 90 % of glycolipid in the testis also consists of a unique glyceroglycolipid, seminolipid. Thus, the lipid has important functions during spermatogenesis.

Related Pathway
KEGG ORTHOLOGY:   
K04628
KEGG Pathway:   
ko00565  ko00600  ko01100 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q16880 
NCBI GeneID:   
7368
GlyMap GeneID:   
7368
DNA aRefSeq:   
NM_001128174.2 
Protein RefSeq:   
NP_001121646.1 
BRENDA:   
Kinetic info.
OMIM number:   
601291
GeneCards database:   
UGT8
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Cer
Product:  
     Gal(b1-r):Cer
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000310836 ENST00000394511
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6887-5p 547-554 -0.486 95.0 -0.421 95.0 noncon
hsa-miR-20a-3p 175-182 -0.478 99.0 -0.451 99.0 noncon
hsa-miR-6795-5p 547-554 -0.478 95.0 -0.414 95.0 noncon
hsa-miR-4703-5p 49-56 -0.474 99.0 -0.453 99.0 noncon
hsa-miR-7109-5p 548-555 -0.47 98.0 -0.407 98.0 noncon
hsa-miR-3942-5p 49-56 -0.468 99.0 -0.447 99.0 noncon
hsa-miR-24-1-5p 1856-1863 -0.43 96.0 -0.363 96.0 noncon
hsa-miR-24-2-5p 1856-1863 -0.43 95.0 -0.363 96.0 noncon
hsa-miR-6514-3p 1771-1778 -0.423 99.0 -0.358 99.0 noncon
hsa-miR-6811-3p 1770-1777 -0.414 98.0 -0.35 98.0 noncon
hsa-miR-1197 393-400 -0.395 99.0 -0.373 99.0 noncon
hsa-miR-3681-3p 1895-1902 -0.395 99.0 -0.335 98.0 con
hsa-miR-218-5p 161-168 -0.394 98.0 -0.372 98.0 con
hsa-miR-544a 177-184 -0.385 98.0 -0.364 98.0 noncon
hsa-miR-361-3p 678-685 -0.382 98.0 -0.332 98.0 noncon
hsa-miR-324-5p 1301-1308 -0.38 96.0 -0.322 96.0 noncon
hsa-miR-216a-3p 1895-1902 -0.377 99.0 -0.32 98.0 con
hsa-miR-4317 1774-1781 -0.37 97.0 -0.314 97.0 noncon
hsa-miR-128-3p 1895-1902 -0.369 98.0 -0.313 98.0 con
hsa-miR-3679-3p 680-687 -0.369 96.0 -0.321 96.0 noncon
hsa-miR-574-5p 449-456 -0.366 96.0 -0.346 96.0 noncon
hsa-miR-24-1-5p 1475-1482 -0.364 93.0 -0.309 94.0 noncon
hsa-miR-6889-3p 591-597 -0.363 97.0 -0.316 97.0 noncon
hsa-miR-6773-3p 1166-1173 -0.359 98.0 -0.306 97.0 noncon
hsa-miR-6862-5p 107-113 -0.356 94.0 -0.337 95.0 noncon
hsa-miR-4288 1651-1658 -0.349 98.0 -0.296 97.0 noncon
hsa-miR-24-2-5p 1475-1482 -0.348 92.0 -0.296 93.0 noncon
hsa-miR-4253 107-113 -0.338 93.0 -0.32 94.0 noncon
hsa-miR-6730-3p 268-275 -0.336 98.0 -0.318 98.0 noncon
hsa-miR-365b-3p 883-889 -0.334 96.0 -0.291 97.0 noncon
hsa-miR-501-5p 468-475 -0.334 98.0 -0.316 98.0 noncon
hsa-miR-365a-3p 883-889 -0.334 96.0 -0.291 97.0 noncon
hsa-miR-4654 1454-1461 -0.325 97.0 -0.276 97.0 noncon
hsa-miR-4711-3p 1766-1773 -0.323 96.0 -0.275 96.0 noncon
hsa-miR-632 1651-1658 -0.313 98.0 -0.266 98.0 noncon
hsa-miR-6731-5p 1584-1591 -0.313 96.0 -0.266 95.0 noncon
hsa-miR-8085 1584-1591 -0.305 95.0 -0.26 94.0 noncon
hsa-miR-5093 972-979 -0.304 98.0 -0.265 98.0 noncon
hsa-miR-4445-3p 1747-1753 -0.302 90.0 -0.257 91.0 noncon
hsa-miR-1260a 1784-1790 -0.301 95.0 -0.256 95.0 noncon
hsa-miR-1260b 1784-1790 -0.301 95.0 -0.256 95.0 noncon
hsa-miR-214-3p 1338-1345 -0.298 98.0 -0.254 97.0 noncon
hsa-miR-20a-3p 1648-1655 -0.298 97.0 -0.254 96.0 noncon
hsa-miR-4769-5p 1454-1461 -0.297 98.0 -0.253 97.0 noncon
hsa-miR-1287-3p 2267-2273 -0.291 93.0 -0.02 51.0 noncon
hsa-miR-526b-5p 453-460 -0.291 97.0 -0.276 97.0 noncon
hsa-miR-4464 166-173 -0.287 98.0 -0.272 98.0 noncon
hsa-miR-4721 1229-1236 -0.285 93.0 -0.243 92.0 noncon
hsa-miR-935 924-931 -0.284 97.0 -0.248 97.0 noncon
hsa-miR-377-3p 512-519 -0.281 95.0 -0.246 95.0 noncon
hsa-miR-586 816-823 -0.277 96.0 -0.242 97.0 noncon
hsa-miR-4427 1962-1969 -0.276 98.0 -0.028 65.0 noncon
hsa-miR-3677-5p 670-676 -0.274 95.0 -0.24 95.0 noncon
hsa-miR-3619-5p 1338-1345 -0.272 97.0 -0.232 96.0 noncon
hsa-miR-4731-5p 1237-1244 -0.268 95.0 -0.228 94.0 noncon
hsa-miR-203a-5p 1180-1186 -0.262 93.0 -0.225 93.0 noncon
hsa-miR-4766-3p 50-56 -0.258 96.0 -0.247 97.0 noncon
hsa-miR-140-3p.1 137-143 -0.257 96.0 -0.243 97.0 noncon
hsa-miR-6852-3p 1796-1802 -0.257 91.0 -0.219 91.0 noncon
hsa-miR-3909 1796-1802 -0.257 91.0 -0.219 91.0 noncon
hsa-miR-6815-3p 234-240 -0.255 93.0 -0.242 94.0 noncon
hsa-miR-3941 347-353 -0.252 97.0 -0.239 97.0 noncon
hsa-miR-4446-5p 1758-1765 -0.252 96.0 -0.215 96.0 noncon
hsa-miR-148b-3p 1861-1867 -0.251 91.0 -0.214 91.0 noncon
hsa-miR-152-3p 1861-1867 -0.251 91.0 -0.214 91.0 noncon
hsa-miR-148a-3p 1861-1867 -0.251 91.0 -0.214 91.0 noncon
hsa-miR-4761-3p 108-114 -0.25 97.0 -0.237 98.0 noncon