EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GFPT1



General Information
Gene Symbol:   
GFPT1
Protein name:   
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.6.1.16
Tissues:   
all, Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   

Fruc(6P) → GlcN(6P)
Reaction (IUBMB):   
No specified reaction.
About

• GFPT 1 / 2 Converts fructose 6-phosphate into D-glucosamine 6 phosphate in the hexosamine biosynthesis pathway with L-glutamate as a substrate.
• Evidence suggests GFPT1 may be inhibited by high UDP-GlcNAc concentrations.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-381038
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q06210 
NCBI GeneID:   
2673
GlyMap GeneID:   
2673
DNA aRefSeq:   
NM_001244710.1 
Protein RefSeq:   
NP_001231639.1 
BRENDA:   
Kinetic info.
OMIM number:   
138292
GeneCards database:   
GFPT1
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     Fruc(6P)
Product:  
     GlcN(6P)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4758-5p 140-147 -0.413 99.0 -0.384 99.0 noncon
hsa-miR-1238-5p 140-147 -0.405 99.0 -0.377 99.0 noncon
hsa-miR-6835-5p 1323-1330 -0.39 97.0 -0.18 76.0 noncon
hsa-miR-6730-3p 1261-1268 -0.39 98.0 -0.182 94.0 noncon
hsa-miR-6782-5p 84-90 -0.386 97.0 -0.384 98.0 noncon
hsa-miR-541-5p 109-116 -0.375 99.0 -0.366 99.0 noncon
hsa-miR-19a-3p 668-675 -0.37 97.0 -0.34 97.0 con
hsa-miR-581 42-49 -0.369 98.0 -0.369 98.0 noncon
hsa-miR-30e-3p 524-531 -0.362 99.0 -0.333 99.0 noncon
hsa-miR-19b-3p 668-675 -0.362 97.0 -0.333 97.0 con
hsa-miR-487b-3p 4269-4275 -0.358 92.0 -0.162 80.0 noncon
hsa-miR-30d-3p 524-531 -0.346 99.0 -0.318 99.0 noncon
hsa-miR-30a-3p 524-531 -0.346 99.0 -0.318 99.0 noncon
hsa-miR-646 889-896 -0.329 98.0 -0.299 98.0 noncon
hsa-miR-223-3p 715-722 -0.326 95.0 -0.3 96.0 con
hsa-miR-4777-3p 1622-1629 -0.324 95.0 -0.151 82.0 noncon
hsa-miR-449c-3p 3232-3239 -0.322 96.0 -0.147 88.0 noncon
hsa-miR-27a-3p 4639-4646 -0.32 97.0 -0.03 69.0 con
hsa-miR-27b-3p 4639-4646 -0.32 97.0 -0.03 69.0 con
hsa-miR-6803-5p 1324-1331 -0.319 96.0 -0.148 83.0 noncon
hsa-miR-3973 6348-6355 -0.317 99.0 -0.014 56.0 noncon
hsa-miR-4660 888-894 -0.312 96.0 -0.283 96.0 noncon
hsa-miR-4524b-5p 890-896 -0.305 94.0 -0.277 95.0 noncon
hsa-miR-4524a-5p 890-896 -0.305 94.0 -0.277 95.0 noncon
hsa-miR-6751-5p 1324-1331 -0.298 95.0 -0.139 83.0 noncon
hsa-miR-3943 6283-6289 -0.296 95.0 -0.013 46.0 noncon
hsa-miR-183-5p.1 3065-3072 -0.295 95.0 -0.135 89.0 noncon
hsa-miR-4771 1918-1925 -0.287 94.0 -0.132 83.0 noncon
hsa-miR-3145-5p 386-393 -0.276 97.0 -0.255 98.0 noncon
hsa-miR-6888-5p 786-793 -0.275 98.0 -0.253 98.0 noncon
hsa-miR-3115 3410-3417 -0.265 91.0 -0.122 80.0 noncon
hsa-miR-4475 1002-1009 -0.264 96.0 -0.142 92.0 noncon
hsa-miR-487a-3p 29-35 -0.263 97.0 -0.263 98.0 noncon
hsa-miR-7973 1687-1693 -0.262 91.0 -0.123 83.0 noncon
hsa-miR-3936 85-91 -0.262 95.0 -0.261 96.0 noncon
hsa-miR-6757-3p 2830-2837 -0.261 97.0 -0.12 89.0 noncon
hsa-miR-4291 843-850 -0.258 95.0 -0.236 95.0 noncon
hsa-miR-154-3p 29-35 -0.252 97.0 -0.252 98.0 noncon
hsa-miR-1910-5p 4206-4213 -0.251 93.0 -0.116 84.0 noncon
hsa-miR-6875-3p 606-613 -0.251 96.0 -0.231 96.0 noncon