EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GANAB



General Information
Gene Symbol:   
GANAB
Protein name:   
Neutral alpha-glucosidase AB
Enzyme Class:   
Glycosidase
CAZy Family:   
GH31
E.C. No:   
3.2.1.207
Organs:   
Reaction (SNFG):   
GANAB GANAB Man(a1-2) GANAB GANAB Glc(a1-3)Man(a1-2)
H2O +
→
+
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• This functions as a heterodimeric enzyme complex GANAB (active part)/PRKCHS (inactive part) to provide a-glucosidase II activity.
• This removes the second and third α(1-3)Glc from Glc2Man9GlcNAc2-Asn during N-glycosylation processing to produce Man9GlcNAc2-Asn.
• In addition to N-glycan processing, α-Glucosidase II is also involved in protein folding quality control in the ER.
• Glycoproteins containing a single α(1-3)Glc residue is recognized by the ER lectin chaperones, calreticulin (CRT) and calnexin (CNX), that are complexed with protein disulfide isomerase isoforms and aid in folding and maturation of glycoproteins.
• Upon transient release from the lectin chaperones, α-Glucosidase II clips the remaining Glc and the protein moves to the next steps of processing provided it is well folded.
• If the glycoprotein is incompletely folded, the misfolded protein is recognized in the context of its N-glycan by the folding sensor UGGT. UGGT puts back one α(1-3)Glc that is removed by GANAB, and refolding is again attempted by CNX/CRT.
• This enzyme activity is inhibited by 1-deoxynojirimycin, N-5-carboxypentyl-dNM, and castanospermine.
• Mutations in PRKCSH were detected in patients with autosomal dominant polycystic liver disease (ADPLD).

Related Pathway
KEGG ORTHOLOGY:   
K05546
KEGG Pathway:   
ko00510  ko01100  ko04141 
Reactome:   
R-HSA-532668
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q14697 
NCBI GeneID:   
23193
GlyMap GeneID:   
23193
DNA aRefSeq:   
NM_001278192.1 
Protein RefSeq:   
NP_001265121.1 
BRENDA:   
Kinetic info.
OMIM number:   
104160
GeneCards database:   
GANAB
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER, Cis, Medial, Trans, TGN
Substrate:  
     Glc(a1-3)Man(a1-2) or Glc(a1-3)Glc(a1-3)Man(a1-2)
Product:  
     Man(a1-2) or Glc(a1-3)Man(a1-2)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 700 600 500 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6889-5p 596-603 -0.666 99.0 -0.605 98.0 noncon
hsa-miR-6777-5p 596-603 -0.66 99.0 -0.6 98.0 noncon
hsa-miR-193a-5p 111-118 -0.596 99.0 -0.596 99.0 con
hsa-miR-188-5p 970-977 -0.551 99.0 -0.082 83.0 noncon
hsa-miR-6786-5p 491-498 -0.515 98.0 -0.47 98.0 noncon
hsa-miR-6866-3p 970-977 -0.486 99.0 -0.073 87.0 noncon
hsa-miR-584-5p 978-985 -0.471 99.0 -0.004 31.0 noncon
hsa-miR-4665-5p 227-234 -0.441 97.0 -0.404 98.0 noncon
hsa-miR-6870-5p 333-340 -0.438 96.0 -0.401 95.0 noncon
hsa-miR-7515 218-225 -0.432 99.0 -0.425 99.0 noncon
hsa-miR-7111-5p 333-340 -0.422 95.0 -0.387 95.0 noncon
hsa-miR-5698 333-340 -0.422 95.0 -0.387 95.0 noncon
hsa-miR-4723-5p 333-340 -0.422 95.0 -0.387 95.0 noncon
hsa-miR-383-3p 43-50 -0.422 99.0 -0.422 99.0 noncon
hsa-miR-4667-5p 85-92 -0.408 98.0 -0.408 99.0 noncon
hsa-miR-625-5p 226-233 -0.403 96.0 -0.369 96.0 noncon
hsa-miR-8089 85-92 -0.403 98.0 -0.403 99.0 noncon
hsa-miR-637 335-342 -0.398 98.0 -0.365 98.0 noncon
hsa-miR-591 775-782 -0.398 97.0 -0.063 65.0 noncon
hsa-miR-4456 431-438 -0.393 98.0 -0.36 98.0 noncon
hsa-miR-4749-3p 731-738 -0.393 97.0 -0.36 97.0 noncon
hsa-miR-4700-5p 85-92 -0.382 98.0 -0.382 99.0 noncon
hsa-miR-6877-5p 78-84 -0.373 94.0 -0.373 95.0 noncon
hsa-miR-6756-3p 30-37 -0.368 96.0 -0.368 97.0 noncon
hsa-miR-3127-3p 30-37 -0.36 96.0 -0.36 97.0 noncon
hsa-miR-6805-5p 711-717 -0.354 94.0 -0.325 94.0 noncon
hsa-miR-571 209-215 -0.352 97.0 -0.346 97.0 noncon
hsa-miR-8085 190-197 -0.352 97.0 -0.348 97.0 noncon
hsa-miR-6731-5p 190-197 -0.352 97.0 -0.348 97.0 noncon
hsa-miR-640 535-542 -0.35 98.0 -0.321 98.0 noncon
hsa-miR-4749-3p 631-638 -0.345 95.0 -0.317 96.0 noncon
hsa-miR-6825-5p 83-90 -0.339 92.0 -0.339 94.0 noncon
hsa-miR-3150b-3p 181-188 -0.336 98.0 -0.332 98.0 noncon
hsa-miR-1275 227-234 -0.336 97.0 -0.308 97.0 noncon
hsa-miR-6778-5p 192-198 -0.335 95.0 -0.331 96.0 noncon
hsa-miR-6755-5p 583-589 -0.335 95.0 -0.308 95.0 noncon
hsa-miR-3132 582-588 -0.331 92.0 -0.304 93.0 noncon
hsa-miR-149-3p 426-433 -0.322 97.0 -0.296 96.0 noncon
hsa-miR-6864-5p 219-225 -0.321 97.0 -0.316 98.0 noncon
hsa-miR-4784 181-188 -0.32 97.0 -0.316 98.0 noncon
hsa-miR-1298-3p 115-121 -0.306 96.0 -0.306 97.0 noncon
hsa-miR-3127-5p 325-332 -0.305 95.0 -0.28 95.0 noncon
hsa-miR-211-5p 969-975 -0.303 97.0 -0.048 75.0 noncon
hsa-miR-4745-5p 194-200 -0.298 93.0 -0.295 94.0 noncon
hsa-miR-6720-5p 845-852 -0.292 96.0 -0.047 70.0 noncon
hsa-miR-4683 179-185 -0.292 96.0 -0.289 97.0 noncon
hsa-miR-204-5p 969-975 -0.292 96.0 -0.047 75.0 noncon
hsa-miR-1233-5p 192-198 -0.291 93.0 -0.288 94.0 noncon
hsa-miR-6883-5p 426-433 -0.29 96.0 -0.267 95.0 noncon
hsa-miR-6785-5p 426-433 -0.288 95.0 -0.265 95.0 noncon
hsa-miR-4728-5p 426-433 -0.288 95.0 -0.265 95.0 noncon
hsa-miR-552-3p 781-788 -0.284 95.0 -0.046 68.0 noncon
hsa-miR-8085 442-449 -0.273 93.0 -0.251 93.0 noncon
hsa-miR-7160-3p 288-294 -0.272 94.0 -0.25 94.0 noncon
hsa-miR-483-3p.2 543-550 -0.269 96.0 -0.248 97.0 con
hsa-miR-6512-3p 845-852 -0.268 95.0 -0.044 69.0 noncon
hsa-miR-766-5p 461-468 -0.267 95.0 -0.246 95.0 noncon
hsa-miR-4660 613-619 -0.264 94.0 -0.243 94.0 noncon
hsa-miR-1233-3p 408-414 -0.262 95.0 -0.241 96.0 noncon
hsa-miR-597-5p 939-945 -0.262 94.0 -0.043 67.0 noncon
hsa-miR-4481 194-200 -0.258 94.0 -0.255 95.0 noncon
hsa-miR-7106-3p 34-40 -0.257 92.0 -0.257 94.0 noncon
hsa-miR-6731-5p 442-449 -0.257 92.0 -0.236 93.0 noncon
hsa-miR-371b-3p 734-740 -0.255 92.0 -0.235 93.0 noncon