EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
UGGT1



General Information
Gene Symbol:   
UGGT1
Protein name:   
UDP-glucose:glycoprotein glucosyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT24
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• UGGT is a large soluble protein that is mostly ER retained.
• This is the glycoprotein folding sensor enzyme. If the glycoprotein is incompletely folded, the misfolded protein is recognized in the context of its N-glycan by the folding sensor UGGT. UGGT puts back one α(1-3)Glc, and refolding is again attempted by CNX/CRT.
• Proteins unable to properly fold after multiple cycles are eventually retro-translocated to the cytosol to be degraded by the proteasome, a process known as endoplasmic reticulum-associated degradation (ERAD).
• It is currently thought that the N-terminal may bind the misfolded polypeptide, while the C-terminal performs glycosyltransferase activity. In humans there are two proteins mediating this enzyme activity.
• Although extensively distributed in nature, UGGT is not required for single cell viability under normal conditions.

Related Pathway
KEGG ORTHOLOGY:   
K11718
KEGG Pathway:   
ko04141 
Reactome:   
R-HSA-901032
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NYU2 
NCBI GeneID:   
56886
GlyMap GeneID:   
56886
DNA aRefSeq:   
NM_020120.3 
Protein RefSeq:   
NP_064505.1 
BRENDA:   
Kinetic info.
OMIM number:   
605897
GeneCards database:   
UGGT1
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Man(a1-2)Man(a1-2)Man(a1-3)
Product:  
     Glc(a1-3)Man(a1-2)Man(a1-2)Man(a1-3)
Constraints:  
     nMan(a1-?)<9
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 60 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6761-3p 984-991 -0.43 94.0 -0.171 80.0 noncon
hsa-miR-4460 2599-2606 -0.352 95.0 -0.14 82.0 noncon
hsa-miR-1224-3p 5680-5687 -0.35 98.0 -0.055 78.0 noncon
hsa-miR-4746-3p 960-967 -0.339 94.0 -0.137 80.0 noncon
hsa-miR-1260b 5681-5688 -0.331 96.0 -0.052 67.0 noncon
hsa-miR-1260a 5681-5688 -0.315 95.0 -0.05 66.0 noncon
hsa-miR-6737-5p 23-29 -0.305 95.0 -0.305 96.0 noncon
hsa-miR-6071 5581-5588 -0.291 97.0 -0.047 68.0 noncon
hsa-miR-6819-5p 23-29 -0.283 93.0 -0.283 94.0 noncon
hsa-miR-4780 4160-4166 -0.26 94.0 -0.052 63.0 noncon
hsa-miR-124-5p 3064-3071 -0.26 92.0 -0.052 58.0 noncon