EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HYAL2



General Information
Gene Symbol:   
HYAL2
Protein name:   
Enzyme Class:   
Glycosidase
CAZy Family:   
GH56
E.C. No:   
3.2.1.35
Organs:   
Reaction (SNFG):   
HYAL2
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Hyaluronanidases cleave glycosidic β1-4 linkages between glucuronic acid and N-acetylglucosamine residues in the hyaluronan polymer.
• Evidence is mixed as to the role hyaluronanidases play in the context of promoting cancer. HYAL protein expression has been correlated to cancer survival and disease progression for various cancer types, such as bladder and pancreatic.
• HYAL2 is traditionally thought to be critical for cell surface degradation of HA, though TMEM2 is now also considered to facilitate this process.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-2160916
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q12891 
NCBI GeneID:   
8692
GlyMap GeneID:   
8692
DNA aRefSeq:   
NM_003773.4 
Protein RefSeq:   
NP_003764.3 
BRENDA:   
Kinetic info.
OMIM number:   
603551
GeneCards database:   
HYAL2
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     GlcNAc(b1-4)GlcA(b1-3)
Product:  
     GlcA(b1-3)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4290 123-130 -0.491 98.0 -0.491 98.0 noncon
hsa-miR-4451 33-40 -0.456 98.0 -0.456 98.0 noncon
hsa-miR-4536-5p 35-42 -0.432 97.0 -0.432 98.0 noncon
hsa-miR-1178-5p 239-246 -0.415 98.0 -0.415 99.0 noncon
hsa-miR-6846-5p 106-113 -0.386 94.0 -0.386 96.0 noncon
hsa-miR-6848-5p 106-113 -0.386 94.0 -0.386 95.0 noncon
hsa-miR-4763-3p 195-202 -0.386 98.0 -0.386 98.0 noncon
hsa-miR-1207-5p 195-202 -0.384 98.0 -0.384 98.0 noncon
hsa-miR-1915-3p 175-182 -0.38 99.0 -0.38 99.0 noncon
hsa-miR-6798-5p 148-154 -0.375 97.0 -0.375 98.0 noncon
hsa-miR-6764-5p 175-182 -0.375 99.0 -0.375 99.0 noncon
hsa-miR-6826-3p 203-210 -0.365 96.0 -0.365 97.0 noncon
hsa-miR-6734-3p 206-213 -0.355 97.0 -0.355 98.0 noncon
hsa-miR-6887-3p 164-171 -0.319 92.0 -0.319 94.0 noncon
hsa-miR-939-3p 118-125 -0.311 97.0 -0.311 97.0 noncon
hsa-miR-342-5p 144-150 -0.307 94.0 -0.307 96.0 noncon
hsa-miR-1233-3p 43-49 -0.297 96.0 -0.297 97.0 noncon
hsa-miR-4664-5p 144-150 -0.295 94.0 -0.295 96.0 noncon
hsa-miR-4667-3p 210-216 -0.289 96.0 -0.289 97.0 noncon
hsa-miR-6868-5p 197-204 -0.28 96.0 -0.28 97.0 noncon
hsa-miR-6778-3p 179-185 -0.27 95.0 -0.27 96.0 noncon
hsa-miR-7848-3p 212-218 -0.264 92.0 -0.264 94.0 noncon
hsa-miR-133a-5p 135-141 -0.263 93.0 -0.263 95.0 noncon
hsa-miR-1343-3p 110-117 -0.256 95.0 -0.256 96.0 noncon
hsa-miR-6826-3p 165-172 -0.252 91.0 -0.252 93.0 noncon
hsa-miR-6852-5p 109-116 -0.251 95.0 -0.251 96.0 noncon
hsa-miR-4718 216-222 -0.25 91.0 -0.25 94.0 noncon