EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MAN1B1



General Information
Gene Symbol:   
MAN1B1
Protein name:   
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Enzyme Class:   
Glycosidase
CAZy Family:   
GH47
E.C. No:   
3.2.1.113
Organs:   
Reaction (SNFG):   
MAN1B1
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Cleavage of four α1,2-mannose (α1,2-Man) residues from Man9GlcNAc2-Asn.
• ER resident Man1B1 first cleaves the single α1,2Man residue from the B branch of the Man9GlcNAc2-Asn.
• This intermediate is then rapidly cleaved in the Golgi by the concerted action of Golgi resident α-mannosidases IA, IB, and IC depending on the relative expression levels in specific cell types.
• Cleavage of Man by these enzymes is followed by the addition of GlcNAc by MGAT1.

Related Pathway
KEGG ORTHOLOGY:   
K01230
KEGG Pathway:   
ko00510  ko00513  ko01100  ko04141 
Reactome:   
R-HSA-4793950
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9UKM7 
NCBI GeneID:   
11253
GlyMap GeneID:   
11253
DNA aRefSeq:   
NM_016219.4 
Protein RefSeq:   
NP_057303.2 
BRENDA:   
Kinetic info.
OMIM number:   
604346
GeneCards database:   
MAN1B1
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Man(a1-2)Man(a1-3)...Man(a1-6)
Product:  
     Man(a1-3)...Man(a1-6)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.00e-04    (pmol/cell • hr)       KM:    1.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 180 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-187-3p 239-246 -0.662 99.0 -0.662 99.0 noncon
hsa-miR-125b-5p 470-477 -0.452 98.0 -0.45 99.0 con
hsa-miR-4319 470-477 -0.444 98.0 -0.442 99.0 con
hsa-miR-125a-5p 470-477 -0.444 98.0 -0.442 99.0 con
hsa-miR-6075 286-293 -0.419 96.0 -0.417 97.0 noncon
hsa-miR-6810-5p 386-393 -0.391 98.0 -0.389 98.0 noncon
hsa-miR-3934-3p 270-277 -0.382 98.0 -0.38 98.0 noncon
hsa-miR-6740-3p 236-243 -0.355 97.0 -0.355 97.0 noncon
hsa-miR-4730 92-98 -0.347 94.0 -0.347 95.0 noncon
hsa-miR-516b-5p 232-239 -0.336 97.0 -0.336 98.0 noncon
hsa-miR-6773-5p 71-77 -0.333 96.0 -0.333 97.0 noncon
hsa-miR-6753-5p 317-324 -0.309 96.0 -0.308 97.0 noncon
hsa-miR-6724-5p 71-77 -0.297 94.0 -0.297 96.0 noncon
hsa-miR-4649-3p 377-384 -0.291 98.0 -0.29 98.0 noncon
hsa-miR-3667-5p 555-561 -0.283 96.0 -0.282 97.0 noncon
hsa-miR-3937 221-227 -0.264 92.0 -0.264 94.0 noncon