EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
MANBA



General Information
Gene Symbol:   
MANBA
Protein name:   
Beta-mannosidase
Enzyme Class:   
Glycosidase
CAZy Family:   
GH2
E.C. No:   
3.2.1.25
Tissues:   
all, Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   
MANBA
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Beta mannosidase active in lysosomes.
• Involved in the turnover of N-linked glycans.
• Deficiency in MANBA activity causes beta mannosidosis, which is analogous to alpha mannosidosis.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-6798695
Related Database
Organism:   
Homo sapiens
Uniprot:   
O00462 
NCBI GeneID:   
4126
GlyMap GeneID:   
4126
DNA aRefSeq:   
NM_005908.3 
Protein RefSeq:   
NP_005899.3 
BRENDA:   
Kinetic info.
OMIM number:   
609489
GeneCards database:   
MANBA
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     
Substrate:  
     Man(b1-4)GlcNAc(b1-?)
Product:  
     GlcNAc(b1-?)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1250-5p 51-58 -0.733 99.0 -0.733 99.0 noncon
hsa-miR-4638-5p 91-98 -0.604 99.0 -0.604 99.0 noncon
hsa-miR-3615 93-100 -0.604 98.0 -0.604 98.0 noncon
hsa-miR-4722-3p 328-335 -0.566 99.0 -0.563 99.0 noncon
hsa-miR-6727-3p 328-335 -0.548 99.0 -0.545 99.0 noncon
hsa-miR-5581-5p 66-73 -0.488 98.0 -0.488 99.0 noncon
hsa-miR-4297 66-73 -0.478 98.0 -0.478 99.0 noncon
hsa-miR-218-1-3p 341-348 -0.471 98.0 -0.468 99.0 noncon
hsa-miR-4423-5p 69-76 -0.469 98.0 -0.469 99.0 noncon
hsa-miR-181a-2-3p 20-27 -0.454 99.0 -0.454 99.0 noncon
hsa-miR-5004-5p 190-197 -0.42 98.0 -0.418 98.0 noncon
hsa-miR-6132 299-306 -0.4 97.0 -0.398 98.0 noncon
hsa-miR-6845-3p 83-90 -0.389 98.0 -0.389 98.0 noncon
hsa-miR-661 181-188 -0.389 99.0 -0.387 99.0 noncon
hsa-miR-2276-5p 434-441 -0.389 99.0 -0.387 99.0 noncon
hsa-miR-6836-5p 299-306 -0.379 97.0 -0.377 98.0 noncon
hsa-miR-593-3p 75-82 -0.372 98.0 -0.372 98.0 noncon
hsa-miR-6755-3p 559-565 -0.354 95.0 0.0 0.0 noncon
hsa-miR-410-3p 543-550 -0.347 99.0 -0.345 99.0 noncon
hsa-miR-582-3p 281-288 -0.342 98.0 -0.34 98.0 noncon
hsa-miR-367-5p 71-78 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-1307-3p 92-98 -0.333 98.0 -0.333 98.0 noncon
hsa-miR-6895-3p 95-102 -0.331 97.0 -0.331 98.0 noncon
hsa-miR-642a-5p 435-442 -0.326 98.0 -0.324 98.0 noncon
hsa-miR-4707-5p 209-215 -0.32 93.0 -0.318 95.0 noncon
hsa-miR-499b-3p 364-371 -0.317 98.0 -0.315 99.0 noncon
hsa-miR-499a-3p 364-371 -0.317 98.0 -0.315 99.0 noncon
hsa-miR-6509-3p 21-27 -0.311 96.0 -0.311 97.0 noncon
hsa-miR-4529-5p 295-301 -0.3 93.0 -0.298 95.0 noncon
hsa-miR-6818-3p 76-83 -0.298 97.0 -0.298 98.0 noncon
hsa-miR-3925-3p 58-65 -0.296 95.0 -0.296 96.0 noncon
hsa-miR-664b-5p 246-252 -0.296 91.0 -0.294 94.0 noncon
hsa-miR-5591-3p 501-507 -0.294 95.0 -0.292 97.0 noncon
hsa-miR-7111-3p 62-69 -0.291 96.0 -0.291 97.0 noncon
hsa-miR-3918 298-304 -0.29 95.0 -0.289 96.0 noncon
hsa-miR-744-3p 70-76 -0.274 94.0 -0.274 96.0 noncon
hsa-miR-496.1 403-410 -0.273 94.0 -0.272 96.0 noncon
hsa-miR-659-3p 342-349 -0.269 96.0 -0.268 97.0 noncon
hsa-miR-5591-3p 488-494 -0.266 94.0 -0.265 96.0 noncon
hsa-miR-6508-3p 296-302 -0.259 95.0 -0.258 96.0 noncon
hsa-miR-1224-5p 191-198 -0.255 97.0 -0.254 98.0 noncon