EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG11



General Information
Gene Symbol:   
ALG11
Protein name:   
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT4
E.C. No:   
2.4.1.131
Organs:   
Reaction (SNFG):   
+
→
+ GDP
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• These enzymes sequentially add 5-mannose from GDP-man to generate Man5Glc2-PP-Dol.
• Alg1 is a β1-4Mannosyltransferase.
• Alg2 adds both α(1-3) and α(1-6) Mannose to Man1GlcNAc2-PP-Dol.
• Alg1, Alg2, and Alg11 mannosyltransferases are ER membrane proteins with catalytic domains that face the cytosol and that use GDP-man as the sugar donor for mannosylation.
• Deficiencies in Alg1, Alg2, and Alg11 result in CDG-Ik, CDG-Ii and CDG-Ip, respectively.
• Intermediate glycan built on dolichol phosphate containing 7 monosaccahrides is flipped into the ER lumen. This is further built with 4 Man and 3 Glc.

Related Pathway
KEGG ORTHOLOGY:   
K03844
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q2TAA5 
NCBI GeneID:   
440138
GlyMap GeneID:   
440138
DNA aRefSeq:   
NM_001004127.2 
Protein RefSeq:   
NP_001004127.2 
BRENDA:   
Kinetic info.
OMIM number:   
613666
GeneCards database:   
ALG11
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Man(a1-3)...GlcNAc(a1-?)Dol-PP or Man(a1-2)...GlcNAc(a1-?)Dol-PP
Product:  
     Man(a1-2)Man(a1-3)...GlcNAc(a1-?)Dol-PP or Man(a1-2)Man(a1-2)...GlcNAc(a1-?)Dol-PP
Constraints:  
     nMan<4
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-718 384-391 -0.67 98.0 -0.022 27.0 noncon
hsa-miR-423-3p 879-886 -0.55 97.0 -0.019 20.0 noncon
hsa-miR-3137 1021-1028 -0.522 99.0 -0.018 65.0 noncon
hsa-miR-5186 76-83 -0.518 99.0 -0.018 56.0 noncon
hsa-miR-6762-3p 798-805 -0.473 98.0 -0.017 42.0 noncon
hsa-miR-6878-3p 923-930 -0.393 98.0 -0.014 55.0 noncon
hsa-miR-1294 620-627 -0.382 98.0 -0.014 42.0 noncon
hsa-miR-4802-5p 784-791 -0.372 98.0 -0.014 48.0 noncon
hsa-miR-2115-5p 351-358 -0.365 97.0 -0.013 32.0 noncon
hsa-miR-765 782-789 -0.355 97.0 -0.013 39.0 noncon
hsa-miR-942-5p 940-947 -0.345 98.0 -0.013 51.0 noncon
hsa-miR-6773-3p 806-813 -0.342 97.0 -0.013 56.0 noncon
hsa-miR-7114-5p 594-601 -0.339 98.0 -0.013 43.0 noncon
hsa-miR-5581-5p 744-751 -0.329 94.0 -0.012 42.0 noncon
hsa-miR-5580-3p 52-59 -0.328 99.0 -0.012 67.0 noncon
hsa-miR-4297 744-751 -0.327 95.0 -0.012 46.0 noncon
hsa-miR-6084 384-390 -0.323 94.0 -0.012 28.0 noncon
hsa-miR-3677-5p 511-517 -0.32 97.0 -0.012 54.0 noncon
hsa-miR-1825 1046-1053 -0.302 97.0 -0.011 59.0 noncon
hsa-miR-8086 162-168 -0.296 92.0 -0.011 36.0 noncon
hsa-miR-671-5p 173-180 -0.291 95.0 -0.011 46.0 noncon
hsa-miR-7110-3p 327-334 -0.29 92.0 -0.011 53.0 noncon
hsa-miR-6730-3p 422-429 -0.283 97.0 -0.011 59.0 noncon
hsa-miR-3926 508-515 -0.28 96.0 -0.011 38.0 noncon
hsa-miR-3163 25-32 -0.279 99.0 -0.279 99.0 noncon
hsa-miR-3936 288-294 -0.278 96.0 -0.01 32.0 noncon
hsa-miR-6720-5p 740-747 -0.272 96.0 -0.01 49.0 noncon
hsa-miR-6762-3p 702-709 -0.27 93.0 -0.01 37.0 noncon
hsa-miR-6884-3p 321-328 -0.267 96.0 -0.01 59.0 noncon
hsa-miR-3152-5p 866-873 -0.265 94.0 -0.01 47.0 noncon
hsa-miR-6512-3p 740-747 -0.264 95.0 -0.01 47.0 noncon
hsa-miR-182-5p 275-282 -0.262 93.0 -0.01 33.0 noncon
hsa-miR-3194-3p 671-678 -0.259 95.0 -0.01 49.0 noncon
hsa-miR-647 799-805 -0.251 96.0 -0.01 61.0 noncon
hsa-miR-604 799-805 -0.25 95.0 -0.01 36.0 noncon