EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG12



General Information
Gene Symbol:   
ALG12
Protein name:   
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT22
E.C. No:   
2.4.1.260
Organs:   
Reaction (SNFG):   
+
→
+ P-Dol
Reaction (IUBMB):   
No specified reaction.
About

• Extension of Man5GlcNAc2-P-P-Dol in the ER lumen involves the addition of four Man (by ALG3 [α(1,3)ManT], ALG9 [α(1,2)ManT], ALG12 [α(1,6)ManT] followed by ALG9 [α(1,2)ManT] again) and three Glc residues (by ALG6 [α(1,3)GlcT], ALG8 [α(1,3)GlcT] and ALG10 [α(1,2)GlcT]).
• In all instances the transfer uses either Dol-P-Man or Dol-P-Glc as donor.
• Dol-P-Man is formed from GDP-Man using enzymes DPM1,2,3
• Dol-P-Glc is formed from UDP-Glc using ALG5

Related Pathway
KEGG ORTHOLOGY:   
K03847
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9BV10 
NCBI GeneID:   
79087
GlyMap GeneID:   
79087
DNA aRefSeq:   
NM_024105.3 
Protein RefSeq:   
NP_077010.1 
BRENDA:   
Kinetic info.
OMIM number:   
607144
GeneCards database:   
ALG12
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Man(a1-2)Man(a1-3)Man(a1-6)
Product:  
     Man(a1-2)Man(a1-3)[Man(a1-6)]Man(a1-6)
Constraints:  
     nMan=7&nMan(a1-6)=1
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000330817 ZNF143 BCL6 PPARG TAL1 PPARG TFAP2C PPARG CTCF NFE2 ZBED5 REST USF2 PPARG PRDM14 E2F6 GATA1 REST AR KLF4 ZNF48 ZNF48 CTCFL MYCN BACH1 ZFX NR2F1 SMAD2 NONO TCF3 GATA4 TAF1 TAF1 E2F6 KLF9 EGR1 CEBPD EGR3 ZNF341 TFAP2A ERG GLIS1 GLIS1 PBX3 CTCF FOS NEUROD1 NFYB NFYC E2F6 IRF3 IRF3 SP2 ATF1 RFX5 AR SOX9 SP4 MXI1 MNT EGR2 CTCF MAF MAF E2F4 MAZ KLF15 BCL11B EGR1 KLF12 MYCN USF2 RBPJ SOX17 KLF1 STAT5B TFAP2C USF1 USF1 REST KLF9 CREM CREM SP5 ZNF792 SP3 ZNF35 BCL11B RFXANK PBX3 PBX3 THAP1 ATF1 FOXA3 GATAD1 PPARG PPARG THAP11 NFYC TEAD1 CEBPB ZBTB48 RXRB RUNX3 ZSCAN9
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-648 1544-1551 -1.551 99.0 -0.03 64.0 noncon
hsa-miR-648 993-999 -1.431 99.0 -0.028 63.0 noncon
hsa-miR-648 1136-1142 -1.43 99.0 -0.028 63.0 noncon
hsa-miR-511-3p 3600-3607 -0.929 99.0 -0.01 39.0 noncon
hsa-miR-6867-5p 2226-2233 -0.798 99.0 -0.019 37.0 noncon
hsa-miR-6867-5p 2046-2053 -0.78 99.0 -0.019 37.0 noncon
hsa-miR-6867-5p 972-979 -0.769 99.0 -0.019 37.0 noncon
hsa-miR-6867-5p 1115-1122 -0.765 99.0 -0.019 37.0 noncon
hsa-miR-6867-5p 937-944 -0.764 99.0 -0.019 37.0 noncon
hsa-miR-6867-5p 1080-1087 -0.76 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 1252-1259 -0.756 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 1975-1982 -0.754 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 1491-1498 -0.75 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 1526-1533 -0.75 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 1697-1704 -0.749 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 2224-2231 -0.747 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 1870-1877 -0.746 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 1658-1665 -0.745 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 2135-2142 -0.743 99.0 -0.018 37.0 noncon
hsa-miR-3650 1969-1976 -0.743 99.0 -0.018 50.0 noncon
hsa-miR-3650 2168-2175 -0.734 99.0 -0.018 50.0 noncon
hsa-miR-6867-5p 2282-2289 -0.732 99.0 -0.018 37.0 noncon
hsa-miR-6867-5p 840-847 -0.727 99.0 -0.018 37.0 noncon
hsa-miR-3650 2315-2322 -0.726 99.0 -0.018 50.0 noncon
hsa-miR-3650 931-938 -0.724 99.0 -0.018 50.0 noncon
hsa-miR-3650 1074-1081 -0.72 99.0 -0.018 50.0 noncon
hsa-miR-3650 1349-1356 -0.72 99.0 -0.018 50.0 noncon
hsa-miR-3650 1178-1185 -0.716 99.0 -0.018 50.0 noncon
hsa-miR-6867-5p 1767-1774 -0.715 99.0 -0.018 36.0 noncon
hsa-miR-3650 892-899 -0.714 99.0 -0.018 50.0 noncon
hsa-miR-3650 1652-1659 -0.711 99.0 -0.018 50.0 noncon
hsa-miR-3650 1485-1492 -0.71 99.0 -0.018 50.0 noncon
hsa-miR-3650 1691-1698 -0.71 99.0 -0.018 50.0 noncon
hsa-miR-4793-3p 2513-2519 -0.71 99.0 -0.018 60.0 noncon
hsa-miR-3650 1246-1253 -0.71 99.0 -0.018 50.0 noncon
hsa-miR-3650 1864-1871 -0.706 99.0 -0.017 50.0 noncon
hsa-miR-3650 1035-1042 -0.704 99.0 -0.017 50.0 noncon
hsa-miR-3650 1615-1622 -0.704 99.0 -0.017 50.0 noncon
hsa-miR-3650 1446-1453 -0.704 99.0 -0.017 50.0 noncon
hsa-miR-3650 2259-2266 -0.702 99.0 -0.017 50.0 noncon
hsa-miR-3650 2117-2124 -0.702 99.0 -0.017 50.0 noncon
hsa-miR-6867-5p 1286-1292 -0.702 99.0 -0.017 36.0 noncon
hsa-miR-6867-5p 1937-1943 -0.688 99.0 -0.017 36.0 noncon
hsa-miR-6867-5p 2008-2014 -0.683 99.0 -0.017 36.0 noncon
hsa-miR-6867-5p 1801-1807 -0.682 99.0 -0.017 36.0 noncon
hsa-miR-491-3p 2393-2400 -0.663 99.0 -0.017 42.0 noncon
hsa-miR-6867-5p 1904-1910 -0.662 99.0 -0.017 36.0 noncon
hsa-miR-3650 843-850 -0.659 99.0 -0.016 49.0 noncon
hsa-miR-3650 2197-2204 -0.647 99.0 -0.016 49.0 noncon
hsa-miR-4455 3424-3430 -0.638 99.0 -0.007 28.0 noncon
hsa-miR-3650 822-829 -0.626 99.0 -0.016 48.0 noncon
hsa-miR-3650 1572-1579 -0.62 99.0 -0.016 48.0 noncon
hsa-miR-3650 2356-2363 -0.615 99.0 -0.016 48.0 noncon
hsa-miR-3650 2302-2309 -0.609 99.0 -0.015 48.0 noncon
hsa-miR-3650 1995-2002 -0.595 99.0 -0.015 48.0 noncon
hsa-miR-3650 1956-1963 -0.592 99.0 -0.015 48.0 noncon
hsa-miR-3650 1233-1240 -0.585 99.0 -0.015 48.0 noncon
hsa-miR-3650 918-925 -0.584 99.0 -0.015 48.0 noncon
hsa-miR-3650 2027-2034 -0.581 99.0 -0.015 48.0 noncon
hsa-miR-3650 1061-1068 -0.579 99.0 -0.015 48.0 noncon
hsa-miR-3650 1639-1646 -0.576 99.0 -0.015 47.0 noncon
hsa-miR-3650 1336-1343 -0.573 99.0 -0.015 47.0 noncon
hsa-miR-3650 1403-1410 -0.573 99.0 -0.015 47.0 noncon
hsa-miR-3650 1165-1172 -0.571 99.0 -0.015 47.0 noncon
hsa-miR-3650 1678-1685 -0.571 99.0 -0.015 47.0 noncon
hsa-miR-3650 1373-1380 -0.571 99.0 -0.015 47.0 noncon
hsa-miR-3650 1022-1029 -0.569 99.0 -0.015 47.0 noncon
hsa-miR-3650 1472-1479 -0.567 99.0 -0.015 47.0 noncon
hsa-miR-3650 1851-1858 -0.565 99.0 -0.015 47.0 noncon
hsa-miR-3650 1602-1609 -0.565 99.0 -0.015 47.0 noncon
hsa-miR-3650 879-886 -0.564 99.0 -0.014 47.0 noncon
hsa-miR-3650 1433-1440 -0.563 99.0 -0.014 47.0 noncon
hsa-miR-3650 1748-1755 -0.562 99.0 -0.014 47.0 noncon
hsa-miR-3650 2246-2253 -0.556 99.0 -0.014 47.0 noncon
hsa-miR-3650 1762-1768 -0.555 99.0 -0.014 47.0 noncon
hsa-miR-3650 2156-2162 -0.551 99.0 -0.014 47.0 noncon
hsa-miR-3650 2105-2111 -0.523 98.0 -0.014 47.0 noncon
hsa-miR-7112-5p 406-413 -0.521 98.0 -0.521 98.0 noncon
hsa-miR-4532 219-226 -0.493 97.0 -0.493 98.0 noncon
hsa-miR-6811-3p 516-523 -0.471 99.0 -0.471 99.0 noncon
hsa-miR-296-5p 198-205 -0.456 97.0 -0.456 97.0 noncon
hsa-miR-1199-3p 470-477 -0.44 97.0 -0.44 98.0 noncon
hsa-miR-8082 530-537 -0.43 99.0 -0.43 99.0 noncon
hsa-miR-5700 743-749 -0.421 99.0 -0.011 58.0 noncon
hsa-miR-92b-5p 3080-3087 -0.413 93.0 -0.011 18.0 noncon
hsa-miR-6867-5p 842-848 -0.407 95.0 -0.011 32.0 noncon
hsa-miR-6867-5p 2196-2202 -0.406 95.0 -0.011 32.0 noncon
hsa-miR-6867-5p 898-904 -0.398 95.0 -0.011 32.0 noncon
hsa-miR-933 2152-2159 -0.391 91.0 -0.011 19.0 noncon
hsa-miR-6867-5p 1452-1458 -0.39 94.0 -0.011 32.0 noncon
hsa-miR-6867-5p 1041-1047 -0.39 94.0 -0.011 32.0 noncon
hsa-miR-6758-3p 574-581 -0.389 99.0 -0.389 99.0 noncon
hsa-miR-4486 279-286 -0.386 99.0 -0.386 99.0 noncon
hsa-miR-6867-5p 2383-2389 -0.377 94.0 -0.01 32.0 noncon
hsa-miR-6867-5p 821-827 -0.371 93.0 -0.01 32.0 noncon
hsa-miR-6762-3p 20-27 -0.368 97.0 -0.368 98.0 noncon
hsa-miR-6867-5p 1571-1577 -0.362 93.0 -0.01 31.0 noncon
hsa-miR-6867-5p 849-855 -0.359 93.0 -0.01 31.0 noncon
hsa-miR-4534 530-537 -0.354 99.0 -0.354 99.0 noncon
hsa-miR-6867-5p 991-997 -0.35 92.0 -0.01 31.0 noncon
hsa-miR-492 143-150 -0.35 95.0 -0.35 96.0 noncon
hsa-miR-4679 534-541 -0.349 98.0 -0.349 99.0 noncon
hsa-miR-6867-5p 1134-1140 -0.348 92.0 -0.01 30.0 noncon
hsa-miR-146a-3p 91-98 -0.343 98.0 -0.343 99.0 noncon
hsa-miR-626 433-440 -0.343 95.0 -0.343 96.0 noncon
hsa-miR-342-5p 392-399 -0.338 96.0 -0.338 97.0 noncon
hsa-miR-6867-5p 2355-2361 -0.335 91.0 -0.009 30.0 noncon
hsa-miR-6876-3p 433-440 -0.335 95.0 -0.335 96.0 noncon
hsa-miR-6867-5p 917-923 -0.331 91.0 -0.009 30.0 noncon
hsa-miR-6867-5p 1060-1066 -0.328 90.0 -0.009 30.0 noncon
hsa-miR-4664-5p 392-399 -0.328 96.0 -0.328 96.0 noncon
hsa-miR-6867-5p 1471-1477 -0.324 90.0 -0.009 30.0 noncon
hsa-miR-6867-5p 2301-2307 -0.324 90.0 -0.009 30.0 noncon
hsa-miR-6769a-5p 3235-3242 -0.323 97.0 -0.009 33.0 noncon
hsa-miR-219a-2-3p 87-94 -0.322 97.0 -0.322 98.0 noncon
hsa-miR-3650 899-905 -0.321 96.0 -0.009 42.0 noncon
hsa-miR-3650 1042-1048 -0.32 96.0 -0.009 42.0 noncon
hsa-miR-6853-5p 396-402 -0.318 90.0 -0.318 92.0 noncon
hsa-miR-3650 1453-1459 -0.315 96.0 -0.009 42.0 noncon
hsa-miR-6769b-5p 3235-3242 -0.305 97.0 -0.009 34.0 noncon
hsa-miR-4793-3p 3203-3209 -0.299 98.0 -0.008 49.0 noncon
hsa-miR-6721-5p 360-367 -0.295 94.0 -0.295 95.0 noncon
hsa-miR-7847-3p 157-164 -0.293 93.0 -0.293 95.0 noncon
hsa-miR-6756-5p 364-371 -0.292 90.0 -0.292 92.0 noncon
hsa-miR-661 326-333 -0.291 96.0 -0.291 97.0 noncon
hsa-miR-7978 134-141 -0.288 96.0 -0.288 97.0 noncon
hsa-miR-6766-5p 364-371 -0.287 91.0 -0.287 93.0 noncon
hsa-miR-4258 2765-2772 -0.273 91.0 -0.008 23.0 noncon
hsa-miR-6884-5p 163-170 -0.271 95.0 -0.271 96.0 noncon
hsa-miR-5008-5p 414-420 -0.265 93.0 -0.265 95.0 noncon
hsa-miR-485-5p 163-170 -0.263 95.0 -0.263 96.0 noncon
hsa-miR-6769a-5p 2210-2217 -0.259 95.0 -0.007 31.0 noncon
hsa-miR-654-5p 363-369 -0.25 92.0 -0.25 94.0 noncon