EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG9



General Information
Gene Symbol:   
ALG9
Protein name:   
Alpha-1,2-mannosyltransferase ALG9
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT22
E.C. No:   
2.4.1.259
Organs:   
Reaction (SNFG):   
+
→
+ P-Dol
+
→
+ P-Dol
Reaction (IUBMB):   
No specified reaction.
About

• Extension of Man5GlcNAc2-P-P-Dol in the ER lumen involves the addition of four Man (by ALG3 [α(1,3)ManT], ALG9 [α(1,2)ManT], ALG12 [α(1,6)ManT] followed by ALG9 [α(1,2)ManT] again) and three Glc residues (by ALG6 [α(1,3)GlcT], ALG8 [α(1,3)GlcT] and ALG10 [α(1,2)GlcT]).
• In all instances the transfer uses either Dol-P-Man or Dol-P-Glc as donor.
• Dol-P-Man is formed from GDP-Man using enzymes DPM1,2,3
• Dol-P-Glc is formed from UDP-Glc using ALG5

Related Pathway
KEGG ORTHOLOGY:   
K03846
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9H6U8 
NCBI GeneID:   
79796
GlyMap GeneID:   
79796
DNA aRefSeq:   
NM_001077690.1 
Protein RefSeq:   
NP_001071158.1 
BRENDA:   
Kinetic info.
OMIM number:   
606941
GeneCards database:   
ALG9
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Man(a1-3)Man(a1-6) or Man(a1-6)...Man(a1-6)
Product:  
     Man(a1-2)Man(a1-3)Man(a1-6) or Man(a1-2)Man(a1-6)...Man(a1-6)
Constraints:  
     nMan>6&nMan(a1-6)=2
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-494-5p 23-30 -0.565 99.0 -0.565 99.0 noncon
hsa-miR-410-5p 23-30 -0.549 99.0 -0.549 99.0 noncon
hsa-miR-323b-5p 23-30 -0.549 99.0 -0.549 99.0 noncon
hsa-miR-513a-5p 4164-4171 -0.521 99.0 -0.015 56.0 noncon
hsa-miR-1296-5p 234-241 -0.513 98.0 -0.037 44.0 noncon
hsa-miR-6866-5p 651-658 -0.494 98.0 -0.036 61.0 noncon
hsa-miR-4738-5p 2113-2120 -0.482 97.0 -0.017 32.0 noncon
hsa-miR-6829-5p 3476-3483 -0.46 99.0 -0.014 48.0 noncon
hsa-miR-4512 233-240 -0.447 99.0 -0.033 57.0 noncon
hsa-miR-5008-3p 242-249 -0.414 98.0 -0.031 63.0 noncon
hsa-miR-7151-3p 77-84 -0.411 99.0 -0.411 99.0 noncon
hsa-miR-370-5p 627-634 -0.397 99.0 -0.03 71.0 noncon
hsa-miR-6781-5p 3217-3224 -0.391 91.0 -0.012 19.0 noncon
hsa-miR-5095 77-84 -0.382 98.0 -0.382 99.0 noncon
hsa-miR-1193 627-634 -0.376 98.0 -0.028 68.0 noncon
hsa-miR-6737-3p 242-249 -0.353 97.0 -0.027 61.0 noncon
hsa-miR-6851-5p 532-539 -0.35 96.0 -0.026 48.0 noncon
hsa-miR-4317 1001-1008 -0.33 96.0 -0.018 55.0 noncon
hsa-miR-7157-3p 242-249 -0.329 97.0 -0.025 59.0 noncon
hsa-miR-3164 167-174 -0.329 97.0 -0.026 56.0 noncon
hsa-miR-6765-3p 4031-4038 -0.328 98.0 -0.01 60.0 noncon
hsa-miR-4802-5p 1292-1299 -0.323 97.0 -0.012 46.0 noncon
hsa-miR-6820-3p 167-174 -0.321 97.0 -0.026 55.0 noncon
hsa-miR-6757-3p 2238-2245 -0.321 98.0 -0.012 52.0 noncon
hsa-miR-765 745-752 -0.318 95.0 -0.024 48.0 noncon
hsa-miR-8082 2725-2732 -0.309 96.0 -0.01 38.0 noncon
hsa-miR-187-5p 4189-4196 -0.302 98.0 -0.009 36.0 noncon
hsa-miR-7-5p 4104-4110 -0.302 97.0 -0.009 55.0 con
hsa-miR-6880-5p 434-441 -0.295 93.0 -0.023 46.0 noncon
hsa-miR-7973 1570-1577 -0.292 93.0 -0.011 43.0 noncon
hsa-miR-601 299-305 -0.291 90.0 -0.022 39.0 noncon
hsa-miR-152-5p 315-322 -0.29 95.0 -0.022 47.0 noncon
hsa-miR-1278 1545-1552 -0.288 96.0 -0.011 43.0 noncon
hsa-miR-367-5p 3933-3940 -0.288 95.0 -0.009 49.0 noncon
hsa-miR-7847-3p 3822-3829 -0.28 93.0 -0.009 36.0 noncon
hsa-miR-4419a 2803-2810 -0.28 94.0 -0.009 33.0 noncon
hsa-miR-7162-5p 4059-4066 -0.279 95.0 -0.009 35.0 noncon
hsa-miR-4527 462-468 -0.279 94.0 -0.022 53.0 noncon
hsa-miR-6759-3p 95-101 -0.276 95.0 -0.276 96.0 noncon
hsa-miR-4755-3p 387-394 -0.276 94.0 -0.021 56.0 noncon
hsa-miR-3681-3p 3718-3725 -0.274 96.0 -0.009 46.0 con
hsa-miR-6828-3p 312-319 -0.272 96.0 -0.021 62.0 noncon
hsa-miR-4270 61-67 -0.271 96.0 -0.271 97.0 noncon
hsa-miR-4501 169-175 -0.269 96.0 -0.022 68.0 noncon
hsa-miR-107 4042-4049 -0.268 95.0 -0.009 36.0 con
hsa-miR-103a-3p 4042-4049 -0.268 95.0 -0.009 36.0 con
hsa-miR-4717-3p 750-757 -0.267 97.0 -0.021 64.0 noncon
hsa-miR-6871-3p 125-131 -0.266 95.0 -0.022 58.0 noncon
hsa-miR-6838-3p 569-576 -0.264 95.0 -0.021 52.0 noncon
hsa-miR-216a-3p 3718-3725 -0.264 96.0 -0.009 47.0 con
hsa-miR-516a-3p 4059-4066 -0.263 94.0 -0.009 36.0 noncon
hsa-miR-516b-3p 4059-4066 -0.263 94.0 -0.009 36.0 noncon
hsa-miR-676-3p 787-793 -0.262 96.0 -0.02 49.0 noncon
hsa-miR-1910-5p 1332-1339 -0.26 94.0 -0.01 42.0 noncon
hsa-miR-6857-5p 3327-3334 -0.258 96.0 -0.008 37.0 noncon
hsa-miR-3689d 532-539 -0.256 96.0 -0.02 55.0 noncon
hsa-miR-128-3p 3718-3725 -0.256 96.0 -0.008 47.0 con
hsa-miR-519d-5p 796-803 -0.254 96.0 -0.02 55.0 noncon
hsa-miR-370-5p 52-58 -0.252 95.0 -0.252 96.0 noncon
hsa-miR-1271-3p 3062-3069 -0.252 90.0 -0.008 32.0 noncon
hsa-miR-765 1290-1297 -0.251 91.0 -0.01 36.0 noncon