EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GALNT8



General Information
Gene Symbol:   
GALNT8
Protein name:   
Probable polypeptide N-acetylgalactosaminyltransferase 8
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
CBM13
E.C. No:   
2.4.1.41
Organs:   
Reaction (SNFG):   
+ Ser →
+ UDP
+ Thr →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Localized in Golgi where it catalyzes O-GalNAc glycosylation.

Related Pathway
KEGG ORTHOLOGY:   
K00710
KEGG Pathway:   
ko00512  ko01100 
Reactome:   
R-HSA-913709
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NY28 
NCBI GeneID:   
26290
GlyMap GeneID:   
26290
DNA aRefSeq:   
NM_017417.1 
Protein RefSeq:   
NP_059113.1 
BRENDA:   
Kinetic info.
OMIM number:   
606250
GeneCards database:   
GALNT8
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Ser or Thr
Product:  
     GalNAc(a1-?)Ser or GalNAc(a1-?)Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000252318 SCRT1 RFX2 RUNX1 TP73 ZNF281 ETV7 HOXB13 SOX9 E2F2 PDX1 PDX1 FOS TFAP4
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1258 57-64 -0.543 99.0 -0.543 99.0 noncon
hsa-miR-3160-5p 90-97 -0.525 99.0 -0.525 99.0 noncon
hsa-miR-34c-5p 1257-1264 -0.439 97.0 -0.001 17.0 noncon
hsa-miR-449b-5p 1257-1264 -0.431 97.0 -0.001 17.0 noncon
hsa-miR-34a-5p 1257-1264 -0.413 97.0 -0.001 17.0 noncon
hsa-miR-449a 1257-1264 -0.413 97.0 -0.001 18.0 noncon
hsa-miR-574-5p 2266-2273 -0.395 97.0 -0.001 20.0 noncon
hsa-miR-486-3p 364-371 -0.382 94.0 -0.001 15.0 noncon
hsa-miR-4643 96-103 -0.374 99.0 -0.374 99.0 noncon
hsa-miR-4520-5p 2311-2318 -0.373 92.0 -0.001 12.0 noncon
hsa-miR-3192-5p 65-71 -0.371 99.0 -0.371 99.0 noncon
hsa-miR-6775-3p 697-704 -0.341 95.0 -0.001 16.0 noncon
hsa-miR-4749-3p 804-811 -0.341 95.0 -0.001 27.0 noncon
hsa-miR-653-3p 2338-2345 -0.336 98.0 -0.001 45.0 noncon
hsa-miR-4433b-5p 2496-2503 -0.335 96.0 -0.001 13.0 noncon
hsa-miR-4436b-5p 39-46 -0.324 98.0 -0.324 98.0 noncon
hsa-miR-4755-3p 1358-1365 -0.322 96.0 -0.001 28.0 noncon
hsa-miR-6876-3p 1549-1556 -0.318 94.0 -0.001 16.0 noncon
hsa-miR-1291 697-704 -0.315 94.0 -0.001 18.0 noncon
hsa-miR-6839-3p 2196-2203 -0.305 95.0 -0.001 25.0 noncon
hsa-miR-122-5p 62-68 -0.304 97.0 -0.304 98.0 noncon
hsa-miR-320e 68-74 -0.304 97.0 -0.304 98.0 noncon
hsa-miR-6894-3p 839-846 -0.302 97.0 -0.001 44.0 noncon
hsa-miR-18a-3p 2047-2054 -0.301 97.0 -0.001 28.0 noncon
hsa-miR-6826-3p 3005-3012 -0.298 94.0 -0.001 31.0 noncon
hsa-miR-6834-3p 3013-3019 -0.297 94.0 -0.001 25.0 noncon
hsa-miR-4780 1311-1318 -0.297 96.0 -0.001 16.0 noncon
hsa-miR-3622a-3p 122-128 -0.294 98.0 -0.294 99.0 noncon
hsa-miR-5006-5p 1359-1366 -0.29 95.0 -0.001 24.0 noncon
hsa-miR-4266 56-62 -0.289 93.0 -0.289 95.0 noncon
hsa-miR-3152-3p 59-65 -0.278 96.0 -0.278 97.0 noncon
hsa-miR-626 1549-1556 -0.278 92.0 -0.001 15.0 noncon
hsa-miR-3659 61-67 -0.274 94.0 -0.274 96.0 noncon
hsa-miR-4433b-3p 55-61 -0.273 96.0 -0.273 97.0 noncon
hsa-miR-8085 593-600 -0.273 93.0 -0.001 23.0 noncon
hsa-miR-7150 2636-2643 -0.27 94.0 -0.001 27.0 noncon
hsa-miR-1912 19-25 -0.267 97.0 -0.267 98.0 noncon
hsa-miR-4691-3p 1770-1777 -0.267 96.0 -0.001 41.0 noncon
hsa-miR-219a-1-3p 2740-2747 -0.267 94.0 -0.001 14.0 noncon
hsa-miR-6731-5p 593-600 -0.257 92.0 -0.001 23.0 noncon
hsa-miR-4433b-5p 1315-1321 -0.255 92.0 -0.001 13.0 noncon
hsa-miR-4711-3p 1434-1441 -0.255 94.0 -0.001 24.0 noncon
hsa-miR-4786-3p 47-53 -0.255 94.0 -0.255 96.0 noncon
hsa-miR-4646-3p 2497-2503 -0.254 92.0 -0.001 25.0 noncon
hsa-miR-6839-3p 2685-2692 -0.253 93.0 -0.001 25.0 noncon
hsa-miR-3622b-3p 122-128 -0.25 98.0 -0.25 98.0 noncon