EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GBGT1



General Information
Gene Symbol:   
GBGT1
Protein name:   
Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT6
E.C. No:   
2.4.1.88
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• NOT ACTIVE IN HUMANS
• Forssman heterophilic glycolipid antigen (GalNAcal-3GalNAcbl-3Galal-4Gal?1-4G1c-Cer) is formed by addition of GalNAc(al-3) by GBGT1 ('Forssman glycolipid synthase', FS). This is a globo series GSL that is widely expressed in mammals.
• While the Forssman gene is ubiquitously expressed in humans, the resulting glycolipid product is absent due to two missense mutations: G230S and Q296R. This results in lack of a(1-3)GalNAc activity.
• The lack of such al,3-GalNAc transferase activity & Forssman antigen expression in humans suggests that the activity is dispensable.
• Some Forssman antigens are however exported in certain tumors.

Related Pathway
KEGG ORTHOLOGY:   
K00722
KEGG Pathway:   
ko00603  ko01100 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8N5D6 
NCBI GeneID:   
26301
GlyMap GeneID:   
26301
DNA aRefSeq:   
NM_001282629.1 
Protein RefSeq:   
NP_001269558.1 
BRENDA:   
Kinetic info.
OMIM number:   
606074
GeneCards database:   
GBGT1
Enzyme Specificity
Glycan Type:  
     GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GalNAc(b1-3)Gal(a1-?)
Product:  
     GalNAc(a1-3)GalNAc(b1-3)Gal(a1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4664-3p 252-259 -0.682 99.0 -0.682 99.0 noncon
hsa-miR-4804-5p 24-31 -0.657 99.0 -0.657 99.0 noncon
hsa-miR-6078 239-246 -0.605 99.0 -0.605 99.0 noncon
hsa-miR-668-5p 362-369 -0.594 98.0 -0.594 99.0 noncon
hsa-miR-4664-5p 673-680 -0.527 99.0 -0.527 99.0 noncon
hsa-miR-342-5p 673-680 -0.521 99.0 -0.521 99.0 noncon
hsa-miR-92a-1-5p 589-596 -0.519 98.0 -0.519 98.0 noncon
hsa-miR-4785 446-453 -0.512 95.0 -0.512 96.0 noncon
hsa-miR-4479 469-475 -0.483 95.0 -0.483 96.0 noncon
hsa-miR-4313 748-754 -0.454 97.0 -0.454 98.0 noncon
hsa-miR-199a-5p 105-112 -0.438 98.0 -0.438 98.0 noncon
hsa-miR-199b-5p 105-112 -0.438 98.0 -0.438 98.0 noncon
hsa-miR-6812-5p 674-681 -0.402 97.0 -0.402 98.0 noncon
hsa-miR-4513 234-241 -0.4 98.0 -0.4 99.0 noncon
hsa-miR-5699-5p 729-736 -0.384 98.0 -0.384 99.0 noncon
hsa-miR-6819-5p 674-681 -0.376 97.0 -0.376 97.0 noncon
hsa-miR-362-5p 113-120 -0.376 99.0 -0.376 99.0 noncon
hsa-miR-6737-5p 674-681 -0.376 97.0 -0.376 98.0 noncon
hsa-miR-1281 361-367 -0.374 94.0 -0.374 96.0 noncon
hsa-miR-296-3p 762-769 -0.365 99.0 -0.031 67.0 noncon
hsa-miR-500b-5p 113-120 -0.36 99.0 -0.36 99.0 noncon
hsa-miR-637 621-628 -0.356 97.0 -0.356 98.0 noncon
hsa-miR-6855-3p 234-241 -0.352 97.0 -0.352 98.0 noncon
hsa-miR-6503-5p 208-215 -0.351 96.0 -0.351 97.0 noncon
hsa-miR-4527 208-215 -0.349 96.0 -0.349 97.0 noncon
hsa-miR-184 24-30 -0.345 90.0 -0.345 93.0 noncon
hsa-miR-6887-3p 356-363 -0.342 94.0 -0.342 95.0 noncon
hsa-miR-4505 70-77 -0.342 97.0 -0.342 97.0 noncon
hsa-miR-6715b-5p 456-463 -0.341 98.0 -0.341 99.0 noncon
hsa-miR-6788-5p 586-593 -0.335 98.0 -0.335 99.0 noncon
hsa-miR-3177-3p 410-416 -0.328 90.0 -0.328 93.0 noncon
hsa-miR-6827-5p 656-662 -0.327 93.0 -0.327 95.0 noncon
hsa-miR-30c-2-3p 586-593 -0.319 98.0 -0.319 99.0 noncon
hsa-miR-30c-1-3p 586-593 -0.319 98.0 -0.319 99.0 noncon
hsa-miR-4693-3p 691-698 -0.314 95.0 -0.314 96.0 noncon
hsa-miR-6736-3p 133-140 -0.311 97.0 -0.311 97.0 noncon
hsa-miR-1273g-5p 591-598 -0.31 97.0 -0.31 98.0 noncon
hsa-miR-873-5p.2 668-675 -0.299 97.0 -0.299 98.0 noncon
hsa-miR-4693-5p 322-329 -0.299 97.0 -0.299 98.0 noncon
hsa-miR-6851-5p 709-715 -0.296 94.0 -0.296 95.0 noncon
hsa-miR-3188 436-443 -0.291 97.0 -0.291 98.0 noncon
hsa-miR-6753-5p 523-529 -0.287 95.0 -0.287 96.0 noncon
hsa-miR-566 534-540 -0.276 93.0 -0.276 95.0 noncon
hsa-miR-6815-5p 707-713 -0.274 94.0 -0.274 96.0 noncon
hsa-miR-4425 588-594 -0.271 90.0 -0.271 93.0 noncon
hsa-miR-1913 731-737 -0.267 95.0 -0.267 96.0 noncon
hsa-miR-6865-5p 707-713 -0.263 94.0 -0.263 96.0 noncon
hsa-miR-4269 456-463 -0.263 98.0 -0.263 98.0 noncon
hsa-miR-324-3p 731-737 -0.257 94.0 -0.257 95.0 noncon
hsa-miR-5787 70-77 -0.256 96.0 -0.256 97.0 noncon
hsa-miR-4505 78-84 -0.25 92.0 -0.25 94.0 noncon