EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GNT8



General Information
Gene Symbol:   
B3GNT8
Protein name:   
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Synthesizes polylacosamine of N-glycan, particulalry along the GlcNAc(β1-6) N-glycan branch.
• B3GnT2 and B3GnT8 may form a heterocomplex to facilitate polyLacNAc extension.
• B3GNT8 is highly expressed in lung, throat, and ileum. It is further augmented during colon cancer.
• Highly metastatic cell lines may synthesize more N-glycans containing polylactosamines than poorly metastatic cell lines.

Related Pathway
KEGG ORTHOLOGY:   
K09665
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-913709
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q7Z7M8 
NCBI GeneID:   
374907
GlyMap GeneID:   
374907
DNA aRefSeq:   
NM_198540.2 
Protein RefSeq:   
NP_940942.1 
BRENDA:   
Kinetic info.
OMIM number:   
615357
GeneCards database:   
B3GNT8
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.70e-06    (pmol/cell • hr)       KM:    7.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 10 8 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-759 219-226 -0.516 99.0 -0.378 99.0 noncon
hsa-miR-7111-5p 134-141 -0.405 94.0 -0.405 95.0 noncon
hsa-miR-4745-5p 217-223 -0.401 97.0 -0.297 95.0 noncon
hsa-miR-6806-5p 184-190 -0.398 94.0 -0.398 96.0 noncon
hsa-miR-4723-5p 134-141 -0.397 94.0 -0.397 95.0 noncon
hsa-miR-4481 217-223 -0.394 98.0 -0.292 96.0 noncon
hsa-miR-6763-5p 214-220 -0.391 98.0 -0.391 98.0 noncon
hsa-miR-5698 134-141 -0.389 94.0 -0.389 95.0 noncon
hsa-miR-3065-3p 21-28 -0.382 98.0 -0.382 99.0 noncon
hsa-miR-6870-5p 134-141 -0.381 93.0 -0.381 94.0 noncon
hsa-miR-491-5p 215-221 -0.377 97.0 -0.28 95.0 noncon
hsa-miR-3150a-3p 214-220 -0.369 97.0 -0.369 98.0 noncon
hsa-miR-504-3p 211-217 -0.352 98.0 -0.352 98.0 noncon
hsa-miR-1296-3p 216-222 -0.349 98.0 -0.26 97.0 noncon
hsa-miR-3175 214-220 -0.343 96.0 -0.343 97.0 noncon
hsa-miR-3661 74-80 -0.342 96.0 -0.342 97.0 noncon
hsa-miR-4666b 207-213 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-4505 138-145 -0.334 96.0 -0.334 97.0 noncon
hsa-miR-631 74-80 -0.321 95.0 -0.321 96.0 noncon
hsa-miR-132-3p 175-181 -0.314 98.0 -0.314 98.0 noncon
hsa-miR-5008-5p 37-43 -0.285 94.0 -0.285 96.0 noncon
hsa-miR-212-3p 175-181 -0.281 97.0 -0.281 98.0 noncon
hsa-miR-5787 138-145 -0.264 96.0 -0.264 97.0 noncon
hsa-miR-455-3p.1 173-179 -0.261 96.0 -0.261 97.0 noncon
hsa-miR-1199-5p 58-64 -0.259 95.0 -0.259 96.0 noncon