EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
POMGNT1



General Information
Gene Symbol:   
POMGNT1
Protein name:   
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT13
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• O-mannosyl glycans are a type of O-glycan in which the reducing terminal mannose is attached to Ser/Thr.
• POMGnT1 catalyzes the transfer of β1,2GlcNAc from UDP-GlcNAc to O-mannose of glycoproteins.
• POMGnT1 deficiency results in muscle-eye-brain disease (MEB), a form of congenital muscular dystrophy/ a-dystroglycanopathy.
• POMGnT1 cannot recognize 6P-Man-peptide as an acceptor. Thus O-mannose phosphorylation occurs after the formation of GlcNAcβ1-2Man linkage.

Related Pathway
KEGG ORTHOLOGY:   
K09666
KEGG Pathway:   
ko00515  ko01100 
Reactome:   
R-HSA-5083628
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8WZA1 
NCBI GeneID:   
55624
GlyMap GeneID:   
55624
DNA aRefSeq:   
NM_017739.3 
Protein RefSeq:   
NP_060209.3 
BRENDA:   
Kinetic info.
OMIM number:   
606822
GeneCards database:   
POMGNT1
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     ER
Substrate:  
     Man(a1-?)Sre|Thr
Product:  
     GlcNAc(b1-2)Man(a1-?)Sre|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6070 205-212 -0.597 97.0 -0.597 97.0 noncon
hsa-miR-4687-5p 96-103 -0.552 99.0 -0.552 99.0 noncon
hsa-miR-4751 173-180 -0.539 99.0 -0.539 99.0 noncon
hsa-miR-4663 19-26 -0.533 99.0 -0.533 99.0 noncon
hsa-miR-7107-5p 1993-2000 -0.526 99.0 -0.525 99.0 noncon
hsa-miR-1234-3p 1993-2000 -0.518 99.0 -0.517 99.0 noncon
hsa-miR-4664-3p 1073-1080 -0.508 97.0 -0.508 97.0 noncon
hsa-miR-615-5p 125-132 -0.497 98.0 -0.497 98.0 noncon
hsa-miR-4749-3p 728-735 -0.477 98.0 -0.477 99.0 noncon
hsa-miR-5586-5p 2195-2202 -0.469 99.0 -0.467 99.0 noncon
hsa-miR-296-5p 2105-2112 -0.457 97.0 -0.456 97.0 noncon
hsa-miR-6812-5p 1586-1593 -0.449 98.0 -0.448 98.0 noncon
hsa-miR-3651 244-251 -0.444 98.0 -0.444 98.0 noncon
hsa-miR-23b-5p 695-702 -0.433 98.0 -0.433 98.0 noncon
hsa-miR-346 47-54 -0.43 97.0 -0.43 98.0 con
hsa-miR-3670 16-23 -0.428 98.0 -0.428 98.0 noncon
hsa-miR-6819-5p 1586-1593 -0.423 98.0 -0.422 98.0 noncon
hsa-miR-3186-3p 767-773 -0.419 92.0 -0.419 95.0 noncon
hsa-miR-1178-5p 416-423 -0.417 98.0 -0.417 99.0 noncon
hsa-miR-6737-5p 1586-1593 -0.407 98.0 -0.406 98.0 noncon
hsa-miR-4726-3p 1880-1887 -0.405 99.0 -0.405 99.0 noncon
hsa-miR-6742-5p 127-134 -0.401 98.0 -0.401 98.0 noncon
hsa-miR-23a-5p 695-702 -0.389 98.0 -0.389 98.0 noncon
hsa-miR-1199-5p 1946-1953 -0.389 98.0 -0.389 99.0 noncon
hsa-miR-4455 131-138 -0.388 95.0 -0.388 96.0 noncon
hsa-miR-93-3p 21-28 -0.385 99.0 -0.385 99.0 noncon
hsa-miR-6512-3p 2027-2034 -0.381 98.0 -0.38 98.0 noncon
hsa-miR-6071 22-29 -0.379 98.0 -0.379 98.0 noncon
hsa-miR-5196-3p 912-919 -0.377 98.0 -0.377 98.0 noncon
hsa-miR-6886-3p 1865-1872 -0.374 96.0 -0.373 97.0 noncon
hsa-miR-6720-5p 2027-2034 -0.373 98.0 -0.373 98.0 noncon
hsa-miR-1224-5p 174-181 -0.37 99.0 -0.37 99.0 con
hsa-miR-558 1904-1911 -0.37 98.0 -0.37 98.0 noncon
hsa-miR-6751-3p 1946-1953 -0.37 98.0 -0.37 98.0 noncon
hsa-miR-2682-3p 28-35 -0.368 98.0 -0.368 98.0 noncon
hsa-miR-2861 2094-2101 -0.361 97.0 -0.361 97.0 noncon
hsa-miR-4467 69-75 -0.356 93.0 -0.356 94.0 noncon
hsa-miR-4638-3p 2179-2186 -0.353 97.0 -0.351 97.0 noncon
hsa-miR-34a-5p 797-804 -0.351 94.0 -0.351 96.0 con
hsa-miR-34c-5p 797-804 -0.345 94.0 -0.345 95.0 con
hsa-miR-3184-3p 1774-1781 -0.342 98.0 -0.342 99.0 noncon
hsa-miR-4717-5p 1064-1071 -0.339 98.0 -0.339 98.0 noncon
hsa-miR-449b-5p 797-804 -0.337 93.0 -0.337 95.0 con
hsa-miR-656-5p 1485-1492 -0.334 94.0 -0.334 96.0 noncon
hsa-miR-3192-5p 159-166 -0.333 98.0 -0.333 99.0 noncon
hsa-miR-6868-5p 1368-1375 -0.331 97.0 -0.331 98.0 noncon
hsa-miR-4487 1253-1260 -0.331 97.0 -0.331 97.0 noncon
hsa-miR-6841-3p 120-127 -0.326 96.0 -0.326 97.0 noncon
hsa-miR-1266-3p 261-268 -0.323 97.0 -0.323 97.0 noncon
hsa-miR-4674 1056-1063 -0.321 97.0 -0.321 98.0 noncon
hsa-miR-449a 797-804 -0.319 92.0 -0.319 95.0 con
hsa-miR-3612 1455-1462 -0.319 97.0 -0.319 98.0 noncon
hsa-miR-6075 1127-1133 -0.315 90.0 -0.315 93.0 noncon
hsa-miR-6781-3p 28-35 -0.315 97.0 -0.315 98.0 noncon
hsa-miR-3922-5p 1982-1989 -0.314 97.0 -0.314 97.0 noncon
hsa-miR-4494 1271-1278 -0.311 97.0 -0.311 98.0 noncon
hsa-miR-4303 1947-1954 -0.31 98.0 -0.31 98.0 noncon
hsa-miR-638 1895-1901 -0.309 97.0 -0.309 97.0 noncon
hsa-miR-8075 749-756 -0.306 95.0 -0.306 96.0 noncon
hsa-miR-6768-3p 1397-1404 -0.305 97.0 -0.305 98.0 noncon
hsa-miR-24-3p 885-892 -0.304 97.0 -0.304 97.0 con
hsa-miR-650 1455-1462 -0.303 97.0 -0.303 98.0 noncon
hsa-miR-6797-5p 1711-1718 -0.299 95.0 -0.299 96.0 noncon
hsa-miR-6871-3p 269-275 -0.299 96.0 -0.299 97.0 noncon
hsa-miR-4665-3p 1995-2001 -0.296 93.0 -0.296 94.0 noncon
hsa-miR-199b-5p 2146-2153 -0.294 94.0 -0.293 96.0 noncon
hsa-miR-4721 2229-2235 -0.293 94.0 -0.291 95.0 noncon
hsa-miR-6773-5p 369-375 -0.293 93.0 -0.293 95.0 noncon
hsa-miR-4645-5p 938-945 -0.291 97.0 -0.291 98.0 noncon
hsa-miR-634 1738-1745 -0.289 96.0 -0.289 97.0 noncon
hsa-miR-6089 1181-1188 -0.287 99.0 -0.287 99.0 noncon
hsa-miR-4755-3p 1942-1948 -0.286 94.0 -0.286 96.0 noncon
hsa-miR-3651 98-104 -0.286 92.0 -0.286 95.0 noncon
hsa-miR-199a-5p 2146-2153 -0.286 94.0 -0.285 95.0 noncon
hsa-miR-5047 117-124 -0.283 96.0 -0.283 97.0 noncon
hsa-miR-1301-3p 117-124 -0.283 96.0 -0.283 97.0 noncon
hsa-miR-4653-3p 1787-1794 -0.282 97.0 -0.282 98.0 noncon
hsa-miR-510-5p 1827-1833 -0.28 96.0 -0.28 97.0 noncon
hsa-miR-663b 1382-1389 -0.277 95.0 -0.277 96.0 noncon
hsa-miR-4656 464-471 -0.277 96.0 -0.277 97.0 noncon
hsa-miR-6803-5p 1607-1614 -0.277 94.0 -0.277 95.0 noncon
hsa-miR-6738-3p 311-318 -0.276 95.0 -0.276 96.0 noncon
hsa-miR-4653-5p 2131-2138 -0.274 97.0 -0.273 98.0 noncon
hsa-miR-1231 162-169 -0.271 97.0 -0.271 98.0 noncon
hsa-miR-4323 1751-1758 -0.268 95.0 -0.268 96.0 noncon
hsa-miR-1249-5p 1711-1718 -0.267 93.0 -0.267 95.0 noncon
hsa-miR-181b-3p 1563-1570 -0.266 97.0 -0.266 98.0 noncon
hsa-miR-3921 2131-2138 -0.266 97.0 -0.265 97.0 noncon
hsa-miR-4437 1055-1062 -0.266 91.0 -0.266 94.0 noncon
hsa-miR-181b-2-3p 1563-1570 -0.266 97.0 -0.266 98.0 noncon
hsa-miR-6802-3p 404-410 -0.265 91.0 -0.265 94.0 noncon
hsa-miR-4673 938-945 -0.265 97.0 -0.265 98.0 noncon
hsa-miR-6878-3p 822-829 -0.265 93.0 -0.265 95.0 noncon
hsa-miR-660-3p 291-298 -0.263 96.0 -0.263 97.0 noncon
hsa-miR-4443 1456-1462 -0.263 93.0 -0.263 95.0 noncon
hsa-miR-512-3p 777-784 -0.262 97.0 -0.262 98.0 noncon
hsa-miR-4457 1350-1357 -0.261 96.0 -0.261 97.0 noncon
hsa-miR-4802-5p 1457-1464 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-218-5p 1224-1231 -0.258 93.0 -0.258 95.0 con
hsa-miR-1200 2064-2071 -0.258 96.0 -0.258 97.0 noncon
hsa-miR-7150 1367-1373 -0.258 94.0 -0.258 95.0 noncon
hsa-miR-3127-5p 148-154 -0.258 92.0 -0.258 94.0 noncon
hsa-miR-103b 99-105 -0.254 91.0 -0.254 94.0 noncon
hsa-miR-103a-2-5p 1221-1228 -0.253 95.0 -0.253 96.0 noncon
hsa-miR-491-5p 128-134 -0.25 91.0 -0.25 93.0 noncon