EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
PAPSS2



General Information
Gene Symbol:   
PAPSS2
Protein name:   
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Enzyme Class:   
CAZy Family:   
E.C. No:   
2.7.7.4
Tissues:   
Muscular, Epithelial, Connective, Nervous
Organs:   
Reaction (SNFG):   

ATP → PAPS
Reaction (IUBMB):   
No specified reaction.
About

• PAPSS1/2 have two functions of phosphorylation of ADP and sulfation of ATP. These are crucial for the formation of PAPS, which is the source for sulfates in glycan modifications.
• PAPSS2 deficiency has been shown to cause physical deformities later in life.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-174362
Related Database
Organism:   
Homo sapiens
Uniprot:   
O95340 
NCBI GeneID:   
9060
GlyMap GeneID:   
9060
DNA aRefSeq:   
NM_001015880.1 
Protein RefSeq:   
NP_001015880.1 
BRENDA:   
Kinetic info.
OMIM number:   
603005
GeneCards database:   
PAPSS2
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     ATP
Product:  
     PAPS
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-124-3p.1 1597-1604 -0.488 99.0 -0.325 97.0 con
hsa-miR-196a-3p 1316-1323 -0.47 99.0 -0.314 97.0 noncon
hsa-miR-8086 865-872 -0.44 97.0 -0.296 95.0 noncon
hsa-miR-212-5p 1320-1327 -0.41 98.0 -0.276 96.0 noncon
hsa-miR-6794-3p 1728-1735 -0.402 98.0 -0.002 35.0 noncon
hsa-miR-6867-5p 160-167 -0.389 94.0 -0.264 88.0 noncon
hsa-miR-493-5p 1688-1695 -0.387 99.0 -0.261 98.0 con
hsa-miR-6867-5p 162-169 -0.386 94.0 -0.262 88.0 noncon
hsa-miR-33b-3p 873-880 -0.386 97.0 -0.262 96.0 noncon
hsa-miR-519e-3p 873-880 -0.383 97.0 -0.26 95.0 noncon
hsa-miR-5699-3p 1584-1591 -0.382 98.0 -0.258 96.0 noncon
hsa-miR-297 173-180 -0.381 98.0 -0.258 96.0 noncon
hsa-miR-124-3p.2 1598-1605 -0.38 95.0 -0.256 90.0 con
hsa-miR-4421 1584-1591 -0.368 97.0 -0.249 95.0 noncon
hsa-miR-224-5p 1618-1625 -0.362 99.0 -0.244 98.0 noncon
hsa-miR-376c-3p 1685-1692 -0.341 98.0 -0.231 95.0 con
hsa-miR-542-3p 623-630 -0.34 98.0 -0.231 95.0 noncon
hsa-miR-6080 875-882 -0.337 96.0 -0.23 94.0 noncon
hsa-miR-506-3p 1598-1605 -0.336 96.0 -0.228 91.0 con
hsa-miR-148a-3p 1192-1199 -0.334 96.0 -0.228 92.0 noncon
hsa-miR-152-3p 1192-1199 -0.334 96.0 -0.228 92.0 noncon
hsa-miR-148b-3p 1192-1199 -0.334 96.0 -0.228 92.0 noncon
hsa-miR-3174 1534-1541 -0.33 98.0 -0.224 97.0 noncon
hsa-miR-4711-5p 1696-1702 -0.318 96.0 -0.216 93.0 noncon
hsa-miR-3149 174-181 -0.318 99.0 -0.217 98.0 noncon
hsa-miR-4735-3p 1344-1350 -0.317 96.0 -0.216 94.0 noncon
hsa-miR-18b-5p 1344-1350 -0.316 96.0 -0.215 94.0 noncon
hsa-miR-6837-3p 1332-1339 -0.312 97.0 -0.212 95.0 noncon
hsa-miR-5197-5p 1001-1008 -0.312 97.0 -0.213 94.0 noncon
hsa-miR-4750-3p 1096-1103 -0.31 96.0 -0.212 94.0 noncon
hsa-miR-18a-5p 1344-1350 -0.305 96.0 -0.208 94.0 noncon
hsa-let-7a-2-3p 423-430 -0.305 98.0 -0.208 96.0 noncon
hsa-miR-3613-5p 1690-1696 -0.301 95.0 -0.205 93.0 noncon
hsa-miR-410-5p 361-367 -0.298 93.0 -0.204 89.0 noncon
hsa-let-7g-3p 423-430 -0.297 98.0 -0.203 96.0 noncon
hsa-miR-3918 812-819 -0.293 95.0 -0.201 92.0 noncon
hsa-miR-374c-5p 71-78 -0.288 97.0 -0.288 98.0 noncon
hsa-miR-515-3p 873-880 -0.281 96.0 -0.193 95.0 noncon
hsa-miR-323b-5p 361-367 -0.276 92.0 -0.189 88.0 noncon
hsa-miR-494-5p 361-367 -0.276 92.0 -0.189 88.0 noncon
hsa-miR-548an 1543-1550 -0.276 97.0 -0.189 94.0 noncon
hsa-miR-142-3p.2 1430-1437 -0.273 96.0 -0.187 93.0 noncon
hsa-miR-655-3p 71-78 -0.272 96.0 -0.272 97.0 noncon
hsa-miR-133a-5p 1560-1566 -0.272 94.0 -0.186 89.0 noncon
hsa-miR-4320 907-914 -0.271 95.0 -0.186 93.0 noncon
hsa-miR-3925-3p 562-569 -0.271 95.0 -0.186 94.0 noncon
hsa-miR-449c-5p 1541-1547 -0.269 98.0 -0.184 96.0 noncon
hsa-miR-34b-5p 1541-1547 -0.269 97.0 -0.184 96.0 noncon
hsa-miR-25-3p 578-585 -0.269 95.0 -0.185 93.0 noncon
hsa-miR-4714-5p 1726-1732 -0.267 96.0 -0.182 93.0 noncon
hsa-miR-363-3p 578-585 -0.266 95.0 -0.183 93.0 noncon
hsa-miR-3907 977-983 -0.265 91.0 -0.182 88.0 noncon
hsa-miR-2278 1650-1657 -0.263 98.0 -0.18 96.0 noncon
hsa-miR-449c-3p 866-873 -0.263 93.0 -0.181 91.0 noncon
hsa-miR-4423-3p 1510-1516 -0.263 93.0 -0.18 90.0 noncon
hsa-miR-610 244-250 -0.262 94.0 -0.18 89.0 noncon
hsa-miR-27b-3p 1397-1403 -0.26 95.0 -0.178 93.0 con
hsa-miR-6734-5p 1106-1112 -0.26 90.0 -0.179 85.0 noncon
hsa-miR-367-3p 578-585 -0.258 94.0 -0.177 92.0 noncon
hsa-miR-1197 413-419 -0.256 96.0 -0.176 94.0 noncon
hsa-miR-5681a 916-923 -0.256 96.0 -0.176 93.0 noncon
hsa-miR-2682-5p 1541-1547 -0.255 97.0 -0.175 96.0 noncon
hsa-miR-203a-3p.1 228-235 -0.253 98.0 -0.174 96.0 con
hsa-miR-5586-3p 457-464 -0.252 96.0 -0.173 93.0 noncon
hsa-miR-3664-3p 1278-1285 -0.252 97.0 -0.173 94.0 noncon