EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
NEU3



General Information
Gene Symbol:   
NEU3
Protein name:   
Sialidase-3
Enzyme Class:   
Glycosidase
CAZy Family:   
GH33
E.C. No:   
3.2.1.18
Organs:   
Reaction (SNFG):   
NEU3 NEU3
H2O +
→
+
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Catalyzes exo-sialidase cleavage of sialic acid on various glycolipids, glycoproteins and oligosacharides.
• Shown to function on α2,3 sialic acid of GD1a & GM3, and α2,8 sialic acid of GD3.
• May also have roles in signaling and regulating endocytosis.
• During N-glycan degradation, oligosaccharides sialidase removal typically precedes activity of α- and β-Galactosidase, β-N-Acetylhexosaminidases, and α-Mannosidases. Manβ1-4GlcNAc is cleaved by β-Mannosidase in the last step.

Related Pathway
KEGG ORTHOLOGY:   
K12357
KEGG Pathway:   
ko00511  ko00600 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9UQ49 
NCBI GeneID:   
10825
GlyMap GeneID:   
10825
DNA aRefSeq:   
NM_006656.5 
Protein RefSeq:   
NP_006647.3 
BRENDA:   
Kinetic info.
OMIM number:   
604617
GeneCards database:   
NEU3
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-?)Gal(b1-?) or NeuAc(a2-?)NeuAc(a2-?)
Product:  
     Gal(b1-?) or NeuAc(a2-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-521 717-724 -0.556 98.0 -0.556 99.0 noncon
hsa-miR-6812-3p 877-884 -0.397 95.0 -0.397 97.0 noncon
hsa-miR-3651 933-940 -0.385 96.0 -0.385 97.0 noncon
hsa-miR-4455 530-536 -0.379 94.0 -0.379 96.0 noncon
hsa-miR-6804-5p 4012-4019 -0.377 96.0 -0.368 96.0 noncon
hsa-miR-15a-3p 4154-4161 -0.362 99.0 -0.354 99.0 noncon
hsa-miR-6822-5p 99-106 -0.355 99.0 -0.355 99.0 noncon
hsa-miR-765 123-130 -0.355 97.0 -0.355 97.0 noncon
hsa-miR-6750-5p 99-106 -0.355 99.0 -0.355 99.0 noncon
hsa-miR-8086 2528-2535 -0.352 94.0 -0.344 96.0 noncon
hsa-miR-376b-5p 526-533 -0.343 96.0 -0.343 97.0 noncon
hsa-miR-199b-5p 816-823 -0.319 95.0 -0.319 96.0 noncon
hsa-miR-585-3p 2217-2224 -0.319 92.0 -0.312 94.0 noncon
hsa-miR-199a-5p 816-823 -0.319 95.0 -0.319 96.0 noncon
hsa-miR-563 3896-3903 -0.316 95.0 -0.309 96.0 noncon
hsa-miR-4443 4273-4280 -0.314 96.0 -0.307 97.0 noncon
hsa-miR-4645-5p 993-1000 -0.313 98.0 -0.313 98.0 noncon
hsa-miR-6738-5p 435-442 -0.313 97.0 -0.313 97.0 noncon
hsa-miR-4323 868-875 -0.312 97.0 -0.312 98.0 noncon
hsa-miR-5194 435-442 -0.308 96.0 -0.308 97.0 noncon
hsa-miR-6881-3p 4387-4394 -0.305 96.0 -0.298 97.0 noncon
hsa-miR-331-3p 407-414 -0.303 97.0 -0.303 98.0 noncon
hsa-miR-3616-3p 843-850 -0.301 93.0 -0.301 95.0 noncon
hsa-miR-7845-5p 1337-1344 -0.294 95.0 -0.287 96.0 noncon
hsa-miR-1295b-3p 3093-3100 -0.291 93.0 -0.284 95.0 noncon
hsa-miR-148a-5p 4398-4405 -0.29 98.0 -0.283 98.0 noncon
hsa-miR-380-5p 3896-3903 -0.29 93.0 -0.283 96.0 noncon
hsa-miR-6861-3p 2463-2470 -0.288 92.0 -0.282 94.0 noncon
hsa-miR-1256 694-701 -0.283 95.0 -0.283 96.0 noncon
hsa-miR-637 1126-1133 -0.282 95.0 -0.282 96.0 noncon
hsa-miR-6752-3p 2404-2411 -0.28 93.0 -0.274 95.0 noncon
hsa-miR-4673 993-1000 -0.279 98.0 -0.279 98.0 noncon
hsa-miR-1273g-3p 163-170 -0.276 97.0 -0.276 98.0 noncon
hsa-miR-649 45-52 -0.276 97.0 -0.276 98.0 noncon
hsa-miR-222-5p 60-67 -0.273 96.0 -0.273 97.0 noncon
hsa-miR-22-3p 2652-2659 -0.272 93.0 -0.266 95.0 noncon
hsa-miR-4722-3p 472-479 -0.271 96.0 -0.271 97.0 noncon
hsa-miR-1295b-3p 1145-1151 -0.267 92.0 -0.267 94.0 noncon
hsa-miR-376c-5p 526-533 -0.262 95.0 -0.262 97.0 noncon
hsa-miR-1255b-5p 650-657 -0.262 92.0 -0.262 94.0 noncon
hsa-miR-1255a 650-657 -0.259 92.0 -0.259 94.0 noncon
hsa-miR-6813-3p 1586-1593 -0.257 95.0 -0.251 97.0 noncon
hsa-miR-7845-5p 4234-4241 -0.254 92.0 -0.248 94.0 noncon
hsa-miR-4423-3p 1650-1657 -0.253 92.0 -0.247 94.0 noncon
hsa-miR-648 1785-1791 -0.253 95.0 -0.247 96.0 noncon
hsa-miR-1226-5p 844-851 -0.251 94.0 -0.251 96.0 noncon
hsa-miR-4741 4124-4131 -0.25 95.0 -0.244 96.0 noncon