EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST6GAL1



General Information
Gene Symbol:   
ST6GAL1
Protein name:   
Beta-galactoside alpha-2,6-sialyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.1
Organs:   
Reaction (SNFG):   
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• Involved in the formation of NeuAc(α2,6)Gal linkages on TypeII lactosamine chains of N-linked glycans
• Ubiquitous enzyme with high expression in liver, lactating mammary gland, hematopoietic activated B cells, and subset of T cells
• ST6Gal I prefers a galactose residue at the α1,3-Man arm of N-linked glycans for addition of the first sialic acid residue.
• SNA and TJA-I bind (α2,6)Sia
• Mice lacking ST6Gal-I display impaired B cell activation and humoral immunity. This may be due to the impairment of Siglec binding, particularly CD22.
• Avian influenza virus HA preferentially recognizes α2,3-sialylated glycans, while human influenza virus HA prefers α2,6-sialylated glycans.

Related Pathway
KEGG ORTHOLOGY:   
K00778
KEGG Pathway:   
ko00510  ko00514  ko01100 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
P15907 
NCBI GeneID:   
6480
GlyMap GeneID:   
6480
DNA aRefSeq:   
NM_003032.2 
Protein RefSeq:   
NP_003023.1 
BRENDA:   
Kinetic info.
OMIM number:   
109675
GeneCards database:   
ST6GAL1
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other)
Compartment:  
     
Substrate:  
     Gal(b1-4)GlcNAc(b1-?)
Product:  
     NeuAc(a2-6)Gal(b1-4)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    7.26e-05    (pmol/cell • hr)       KM:    2.60e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 100 80 60 40 20 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-3189-3p 2702-2709 -0.659 99.0 -0.625 99.0 noncon
hsa-miR-6787-5p 261-267 -0.395 94.0 -0.395 95.0 noncon
hsa-miR-4313 842-849 -0.394 96.0 -0.384 97.0 noncon
hsa-miR-492 164-171 -0.393 97.0 -0.393 98.0 noncon
hsa-miR-6751-5p 1144-1151 -0.393 98.0 -0.383 98.0 noncon
hsa-miR-6803-5p 1144-1151 -0.39 97.0 -0.38 98.0 noncon
hsa-miR-4771 382-389 -0.387 97.0 -0.386 98.0 noncon
hsa-miR-6835-5p 1143-1150 -0.383 96.0 -0.373 97.0 noncon
hsa-miR-7109-5p 3220-3227 -0.381 96.0 0.0 0.0 noncon
hsa-miR-6795-5p 3219-3226 -0.377 90.0 0.0 0.0 noncon
hsa-miR-4437 197-204 -0.37 97.0 -0.37 98.0 noncon
hsa-miR-3944-3p 3496-3502 -0.366 90.0 0.0 0.0 noncon
hsa-miR-644a 214-221 -0.364 97.0 -0.364 97.0 noncon
hsa-miR-346 699-706 -0.363 95.0 -0.354 96.0 noncon
hsa-miR-1908-5p 261-267 -0.359 92.0 -0.359 94.0 noncon
hsa-miR-4645-5p 2980-2987 -0.358 98.0 0.0 0.0 noncon
hsa-miR-663a 261-267 -0.352 91.0 -0.352 92.0 noncon
hsa-miR-5703 17-24 -0.348 98.0 -0.348 98.0 noncon
hsa-miR-4434 17-24 -0.34 98.0 -0.34 98.0 noncon
hsa-miR-876-3p 1162-1169 -0.338 96.0 -0.33 97.0 noncon
hsa-miR-628-5p 120-127 -0.335 98.0 -0.335 98.0 noncon
hsa-miR-626 249-256 -0.334 94.0 -0.334 96.0 noncon
hsa-miR-8082 20-27 -0.334 97.0 -0.334 98.0 noncon
hsa-miR-4533 2617-2624 -0.324 98.0 -0.309 98.0 noncon
hsa-miR-4673 2980-2987 -0.324 98.0 0.0 0.0 noncon
hsa-miR-21-5p 2633-2640 -0.321 97.0 -0.306 98.0 con
hsa-miR-6731-5p 295-302 -0.317 96.0 -0.317 97.0 noncon
hsa-miR-8085 295-302 -0.317 96.0 -0.317 97.0 noncon
hsa-miR-3130-5p 2343-2350 -0.316 98.0 -0.308 98.0 noncon
hsa-miR-4715-5p 2794-2800 -0.308 95.0 0.0 0.0 noncon
hsa-miR-4302 2248-2255 -0.303 97.0 -0.296 98.0 noncon
hsa-miR-6845-5p 1604-1611 -0.303 91.0 -0.296 93.0 noncon
hsa-miR-4482-5p 2343-2350 -0.302 97.0 -0.295 98.0 noncon
hsa-miR-6876-3p 249-256 -0.302 93.0 -0.302 95.0 noncon
hsa-miR-7113-3p 3406-3413 -0.298 97.0 0.0 0.0 noncon
hsa-miR-8052 1117-1124 -0.296 94.0 -0.289 96.0 noncon
hsa-miR-3199 1117-1124 -0.293 94.0 -0.286 95.0 noncon
hsa-miR-378g 199-206 -0.293 97.0 -0.293 98.0 noncon
hsa-miR-3151-3p 818-825 -0.29 94.0 -0.283 96.0 noncon
hsa-miR-509-3p 2624-2631 -0.286 92.0 -0.273 94.0 noncon
hsa-miR-6729-3p 1818-1825 -0.283 94.0 -0.276 95.0 noncon
hsa-miR-3665 3448-3455 -0.277 93.0 0.0 0.0 noncon
hsa-miR-4534 20-27 -0.274 97.0 -0.274 98.0 noncon
hsa-miR-6773-5p 2208-2214 -0.272 92.0 -0.265 94.0 noncon
hsa-miR-1912 2344-2350 -0.269 97.0 -0.262 98.0 noncon
hsa-miR-4659a-5p 880-887 -0.269 95.0 -0.262 96.0 noncon
hsa-miR-7160-3p 1708-1714 -0.267 93.0 -0.261 94.0 noncon
hsa-miR-6511b-3p 2494-2501 -0.266 94.0 -0.259 95.0 noncon
hsa-miR-3909 2812-2819 -0.266 92.0 0.0 0.0 noncon
hsa-miR-6511a-3p 2494-2501 -0.266 94.0 -0.259 95.0 noncon
hsa-miR-8086 1757-1763 -0.265 90.0 -0.259 93.0 noncon
hsa-miR-484 145-151 -0.264 97.0 -0.264 98.0 noncon
hsa-miR-214-5p 3392-3399 -0.263 96.0 0.0 0.0 noncon
hsa-miR-939-5p 1746-1752 -0.263 93.0 -0.257 94.0 noncon
hsa-miR-4793-3p 2281-2288 -0.263 97.0 -0.257 98.0 noncon
hsa-miR-4516 17-24 -0.262 98.0 -0.262 98.0 noncon
hsa-miR-6732-5p 3193-3199 -0.259 90.0 0.0 0.0 noncon
hsa-miR-637 3251-3257 -0.259 93.0 0.0 0.0 noncon
hsa-miR-6724-5p 2208-2214 -0.258 92.0 -0.252 94.0 noncon
hsa-miR-6852-3p 2812-2819 -0.258 91.0 0.0 0.0 noncon
hsa-miR-3663-3p 1898-1905 -0.256 90.0 -0.25 93.0 noncon
hsa-miR-5008-5p 112-118 -0.256 93.0 -0.256 95.0 noncon
hsa-miR-1270 2804-2811 -0.254 96.0 0.0 0.0 noncon
hsa-miR-1295b-5p 2344-2350 -0.254 97.0 -0.248 98.0 noncon
hsa-miR-424-3p 406-413 -0.252 95.0 -0.246 96.0 noncon
hsa-miR-7106-5p 257-263 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-1343-5p 1746-1752 -0.252 93.0 -0.246 94.0 noncon
hsa-miR-3652 88-94 -0.251 96.0 -0.251 97.0 noncon