EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ST8SIA6



General Information
Gene Symbol:   
ST8SIA6
Protein name:   
Alpha-2,8-sialyltransferase 8F
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT29
E.C. No:   
2.4.99.8
Organs:   
Reaction (SNFG):   
+
→
+ CMP
+
→
+ CMP
Reaction (IUBMB):   
No specified reaction.
About

• ST8Sia-VI is involved in the biosynthesis of disialic acid motifs on O-glycans, but not oligo-/poly-Sia.
• The enzyme exhibits low/no activity toward N-glycans and glycolipids; however, it exhibits high activity toward O-glycans of glycoproteins [bovine submaxillary mucin ((GlcNAcβ1-3)NeuAcα2-6GalNAc-O-Ser/Thr) and fetuin (NeuAcα2-3Galβ1-3GalNAc-O-Ser/Thr), and also sialyloligosaccharides:α3/6-sialyllactose and α3/6-sialyl-N-acetyllactosamine.
• Highly expressed in kidney, moderately expressed in the heart and spleen. Several normal and cancer cells express this gene.

Related Pathway
KEGG ORTHOLOGY:   
K06615
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-4085001
Related Database
Organism:   
Homo sapiens
Uniprot:   
P61647 
NCBI GeneID:   
338596
GlyMap GeneID:   
338596
DNA aRefSeq:   
NM_001004470.2 
Protein RefSeq:   
NP_001004470.1 
BRENDA:   
Kinetic info.
OMIM number:   
610139
GeneCards database:   
ST8SIA6
Enzyme Specificity
Glycan Type:  
     O-linked (mucin type)
Compartment:  
     
Substrate:  
     NeuAc(a2-3)Gal(b1-?) or NeuAc(a2-6)...GalNAc(a1-?)Ser|Thr
Product:  
     NeuAc(a2-8)NeuAc(a2-3)Gal(b1-?) or NeuAc(a2-8)NeuAc(a2-6)...GalNAc(a1-?)Ser|Thr
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4283 100-107 -0.537 99.0 -0.537 99.0 noncon
hsa-miR-5702 210-217 -0.528 99.0 -0.528 99.0 noncon
hsa-miR-4260 102-109 -0.516 99.0 -0.516 99.0 noncon
hsa-miR-6509-3p 982-989 -0.513 99.0 -0.513 99.0 noncon
hsa-miR-6807-3p 36-43 -0.507 99.0 -0.507 99.0 noncon
hsa-miR-205-3p 1110-1117 -0.491 99.0 -0.261 99.0 noncon
hsa-miR-217 36-43 -0.486 99.0 -0.486 99.0 noncon
hsa-miR-3162-3p 267-274 -0.47 98.0 -0.47 98.0 noncon
hsa-miR-4747-5p 434-441 -0.466 99.0 -0.466 99.0 noncon
hsa-miR-616-3p 919-926 -0.457 99.0 -0.457 99.0 noncon
hsa-miR-5196-5p 434-441 -0.434 98.0 -0.434 99.0 noncon
hsa-miR-4745-5p 131-138 -0.429 98.0 -0.429 98.0 noncon
hsa-miR-6507-3p 1136-1143 -0.427 99.0 -0.229 97.0 noncon
hsa-miR-6770-5p 166-173 -0.422 99.0 -0.422 99.0 noncon
hsa-miR-4428 185-192 -0.416 99.0 -0.416 99.0 noncon
hsa-miR-4275 1080-1087 -0.411 99.0 -0.221 98.0 noncon
hsa-miR-4720-3p 481-488 -0.403 99.0 -0.403 99.0 noncon
hsa-miR-181a-2-3p 981-988 -0.398 99.0 -0.398 99.0 noncon
hsa-miR-1827 662-669 -0.396 98.0 -0.396 99.0 noncon
hsa-miR-4481 131-138 -0.393 98.0 -0.393 98.0 noncon
hsa-miR-4524a-3p 447-454 -0.367 98.0 -0.367 99.0 noncon
hsa-miR-6836-5p 817-824 -0.366 97.0 -0.366 97.0 noncon
hsa-miR-6885-3p 484-491 -0.353 98.0 -0.353 99.0 noncon
hsa-miR-6780b-5p 127-134 -0.346 96.0 -0.346 97.0 noncon
hsa-miR-6824-5p 1012-1019 -0.343 95.0 -0.187 85.0 noncon
hsa-miR-6132 817-824 -0.339 96.0 -0.339 97.0 noncon
hsa-miR-4725-3p 127-134 -0.338 96.0 -0.338 97.0 noncon
hsa-miR-3916 611-617 -0.326 98.0 -0.326 99.0 noncon
hsa-miR-5695 351-358 -0.318 98.0 -0.318 98.0 noncon
hsa-miR-6880-5p 314-320 -0.316 94.0 -0.316 95.0 noncon
hsa-miR-4299 322-329 -0.315 98.0 -0.315 98.0 noncon
hsa-miR-6842-3p 306-312 -0.311 96.0 -0.311 97.0 noncon
hsa-miR-4437 99-105 -0.31 94.0 -0.31 96.0 noncon
hsa-miR-503-3p 728-735 -0.304 95.0 -0.304 97.0 noncon
hsa-miR-133a-5p 304-310 -0.295 95.0 -0.295 96.0 noncon
hsa-miR-4716-5p 194-201 -0.289 97.0 -0.289 98.0 noncon
hsa-miR-493-5p 999-1006 -0.289 98.0 -0.159 94.0 noncon
hsa-miR-3158-5p 1020-1027 -0.288 98.0 -0.158 94.0 noncon
hsa-miR-5690 570-576 -0.287 98.0 -0.287 98.0 noncon
hsa-miR-4271 127-134 -0.286 96.0 -0.286 97.0 noncon
hsa-miR-3938 18-24 -0.284 95.0 -0.284 97.0 noncon
hsa-miR-208b-5p 926-933 -0.284 98.0 -0.284 98.0 noncon
hsa-miR-4286 1132-1138 -0.283 96.0 -0.155 91.0 noncon
hsa-miR-455-3p.2 457-463 -0.28 97.0 -0.28 98.0 noncon
hsa-miR-3149 216-223 -0.278 99.0 -0.278 99.0 noncon
hsa-miR-6715a-3p 113-120 -0.278 97.0 -0.278 98.0 noncon
hsa-miR-6808-5p 661-667 -0.274 97.0 -0.274 98.0 noncon
hsa-miR-6893-5p 661-667 -0.274 97.0 -0.274 98.0 noncon
hsa-miR-889-5p 307-313 -0.272 95.0 -0.272 97.0 noncon
hsa-miR-4774-5p 303-309 -0.272 94.0 -0.272 96.0 noncon
hsa-miR-135b-5p 599-605 -0.272 94.0 -0.272 95.0 noncon
hsa-miR-135a-5p 599-605 -0.272 94.0 -0.272 95.0 noncon
hsa-miR-140-3p.1 1041-1048 -0.27 97.0 -0.149 90.0 noncon
hsa-miR-3125 611-617 -0.27 97.0 -0.27 98.0 noncon
hsa-miR-487a-3p 904-911 -0.269 97.0 -0.269 98.0 noncon
hsa-miR-20a-3p 35-41 -0.265 95.0 -0.265 97.0 noncon
hsa-miR-30a-3p 1109-1115 -0.258 98.0 -0.142 95.0 noncon
hsa-miR-3909 837-844 -0.258 91.0 -0.258 94.0 noncon
hsa-miR-30e-3p 1109-1115 -0.258 97.0 -0.142 95.0 noncon
hsa-miR-30d-3p 1109-1115 -0.258 97.0 -0.142 95.0 noncon
hsa-miR-8081 273-280 -0.256 94.0 -0.256 96.0 noncon
hsa-miR-491-5p 129-135 -0.254 91.0 -0.254 93.0 noncon