EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
SULF1



General Information
Gene Symbol:   
SULF1
Protein name:   
Extracellular sulfatase Sulf-1
Enzyme Class:   
CAZy Family:   
E.C. No:   
3.1.6.*
Organs:   
Reaction (SNFG):   
→
Reaction (IUBMB):   
No specified reaction.
About

• Extracellular sulfatases 1 and 2 are secreted proteins that remodel the glycosaminoglycans in the extracellular matrix by removing sulfate groups.
• SULF1 and SULF2 specifically act on GlcNAc 6-sulfate, diminishing the amount of heparan sulfate proteoglycans.
• SULF1 overexpression in ovarian cancer confers cisplatin resistance.

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8IWU6 
NCBI GeneID:   
23213
GlyMap GeneID:   
23213
DNA aRefSeq:   
NM_001128204.1 
Protein RefSeq:   
NP_001121676.1 
BRENDA:   
Kinetic info.
OMIM number:   
610012
GeneCards database:   
SULF1
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     GlcNAc(a1-4 [6S])
Product:  
     GlcNAc(a1-4)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 1200 1100 1000 900 800 700 600 500 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-199a-5p 421-428 -0.477 98.0 -0.477 98.0 con
hsa-miR-199b-5p 421-428 -0.461 98.0 -0.461 98.0 con
hsa-miR-1247-5p 444-450 -0.356 92.0 -0.356 94.0 noncon
hsa-miR-1343-5p 1967-1974 -0.329 96.0 -0.329 97.0 noncon
hsa-miR-3605-3p 939-946 -0.327 91.0 -0.327 93.0 noncon
hsa-miR-8081 187-194 -0.324 97.0 -0.324 98.0 noncon
hsa-miR-939-5p 1967-1974 -0.321 96.0 -0.321 96.0 noncon
hsa-miR-2681-3p 2297-2304 -0.321 98.0 -0.321 99.0 noncon
hsa-miR-924 185-192 -0.318 97.0 -0.318 98.0 noncon
hsa-miR-6803-5p 1362-1369 -0.314 95.0 -0.314 97.0 noncon
hsa-miR-6751-5p 1362-1369 -0.309 95.0 -0.309 96.0 noncon
hsa-miR-6834-3p 1076-1083 -0.298 94.0 -0.298 96.0 noncon
hsa-miR-422a 359-366 -0.292 95.0 -0.292 97.0 noncon
hsa-miR-378h 359-366 -0.292 95.0 -0.292 97.0 noncon
hsa-miR-378c 359-366 -0.284 95.0 -0.284 97.0 noncon
hsa-miR-378a-3p 359-366 -0.284 95.0 -0.284 97.0 noncon
hsa-miR-378i 359-366 -0.284 95.0 -0.284 97.0 noncon
hsa-miR-378e 359-366 -0.284 95.0 -0.284 97.0 noncon
hsa-miR-378d 359-366 -0.284 95.0 -0.284 97.0 noncon
hsa-miR-6753-5p 1151-1158 -0.279 95.0 -0.279 96.0 noncon
hsa-miR-378b 359-366 -0.276 95.0 -0.276 96.0 noncon
hsa-miR-493-3p 1818-1825 -0.275 95.0 -0.275 96.0 noncon
hsa-miR-7974 860-867 -0.269 91.0 -0.269 94.0 noncon
hsa-miR-20b-3p 87-94 -0.268 96.0 -0.268 97.0 noncon
hsa-miR-5189-3p 1003-1009 -0.268 93.0 -0.268 95.0 noncon
hsa-miR-1287-3p 1111-1118 -0.268 91.0 -0.268 94.0 noncon
hsa-miR-7162-5p 2260-2267 -0.261 94.0 -0.261 96.0 noncon
hsa-miR-627-5p 186-193 -0.261 96.0 -0.261 97.0 noncon
hsa-miR-378f 359-366 -0.26 94.0 -0.26 96.0 noncon
hsa-miR-892a 811-818 -0.259 96.0 -0.259 97.0 noncon
hsa-miR-4474-5p 458-465 -0.256 93.0 -0.256 95.0 noncon
hsa-miR-5007-5p 2140-2146 -0.254 95.0 -0.254 96.0 noncon
hsa-miR-516a-3p 2260-2267 -0.253 94.0 -0.253 96.0 noncon
hsa-miR-516b-3p 2260-2267 -0.253 94.0 -0.253 96.0 noncon