EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST2



General Information
Gene Symbol:   
CHST2
Protein name:   
Carbohydrate sulfotransferase 2
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Five GlcNAc6ST enzymes have been found in humans.
• GlcNAc6ST-1 (CHST2) catalyzes the formation of 6-sulfo sialyl Lewis-X (6-sulfo sLeX, the major L-selectin ligand on HEV. This is also a major enzyme facilitating C6-GlcNAc sulfation of KS. CHST2 acts at the non-reducing end of GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3).
• GlcNAc6ST-2 (CHST4) is expressed on High Endothelial Cells of the lymph node (HEV). Both CHST2 and CHST4 can make 6-sulfo sialyl Lewis-X. It is also involved in the biosynthesis of MECA-79 epitope. CHST4 acts internally on LacNAc substrate.
• GlcNAc6ST-3 (CHST5) is a GlcNAc6ST that is functional in the intestines. It is highly expressed in colon and small intestine. CHST5 does not act to form KS, but it may attach sulfate to C6 on O-glycan core-2 glycan at the non-reducing end. As it is located in the intestines, it does not participate in the formation of the sulfated sLeX epitope.
• GlcNAc6ST-4/GST-5 (CHST7) is widely expressed in many organs. Less is known about this enzyme. It may contribute to C6 sulfation of GalNAc during chondroitin sulfate biosynthesis.
• GlcNAc6ST-5 (CHST6) is expressed in the cornea, and involved in the sulfation of keratin sulfate GAGs of the eye. The absence of this enzyme results in macular corneal dystrophy.

Related Pathway
KEGG ORTHOLOGY:   
K04745
KEGG Pathway:   
ko00533 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y4C5 
NCBI GeneID:   
9435
GlyMap GeneID:   
9435
DNA aRefSeq:   
NM_004267.4 
Protein RefSeq:   
NP_004258.2 
BRENDA:   
Kinetic info.
OMIM number:   
603798
GeneCards database:   
CHST2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-3)Gal(b1-4) or Gal(b1-3)[GlcNAc(b1-6)]GalNAc(a1-?)Ser|Thr or Gal(b1-3)[Gal(b1-4)GlcNAc(b1-6)]GalNAc(a1-?)Ser|Thr
Product:  
     Gal(b1-4)GlcNAc(b1-3 [6S])Gal(b1-4) or Gal(b1-3)[GlcNAc(b1-6 [6S])]GalNAc(a1-?)Ser|Thr or Gal(b1-3)[Gal(b1-4)GlcNAc(b1-6 [6S])]GalNAc(a1-?)Ser|Thr
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000309575 SMAD4 CTCFL ZSCAN22 PAX5 TBP E2F6 EBF1 EBF1 MYCN USF1 SP2 SKI YY1 TCF7L1 SP4 PBX3 NRF1 NFYB TFAP4 KLF9 VEZF1 SP1 ZNF467 MAZ EGR1 MAX SMAD1 SMAD5 ZBTB7A TAF1 EGR3 TFAP2C EGR2 E2F6 CTCFL MAF
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-639 1144-1151 -0.593 98.0 0.0 0.0 noncon
hsa-miR-6782-5p 192-199 -0.592 99.0 -0.46 99.0 noncon
hsa-miR-378h 74-81 -0.544 99.0 -0.544 99.0 noncon
hsa-miR-378e 74-81 -0.528 99.0 -0.528 99.0 noncon
hsa-miR-378c 74-81 -0.528 99.0 -0.528 99.0 noncon
hsa-miR-378a-3p 74-81 -0.528 99.0 -0.528 99.0 noncon
hsa-miR-378i 74-81 -0.528 99.0 -0.528 99.0 noncon
hsa-miR-422a 74-81 -0.528 99.0 -0.528 99.0 noncon
hsa-miR-4417 95-102 -0.528 99.0 -0.412 99.0 noncon
hsa-miR-378d 74-81 -0.528 99.0 -0.528 99.0 noncon
hsa-miR-378f 74-81 -0.512 99.0 -0.512 99.0 noncon
hsa-miR-378b 74-81 -0.512 99.0 -0.512 99.0 noncon
hsa-miR-648 820-827 -0.509 99.0 0.0 0.0 noncon
hsa-miR-7154-3p 285-292 -0.487 99.0 -0.382 99.0 noncon
hsa-miR-4474-3p 178-185 -0.477 99.0 -0.374 98.0 noncon
hsa-miR-299-3p 97-104 -0.459 99.0 -0.36 98.0 con
hsa-miR-6867-5p 129-136 -0.446 97.0 -0.35 94.0 noncon
hsa-miR-30e-5p 351-358 -0.44 99.0 -0.346 97.0 con
hsa-miR-30d-5p 351-358 -0.424 98.0 -0.334 97.0 con
hsa-miR-30a-5p 351-358 -0.424 98.0 -0.334 97.0 con
hsa-miR-3612 566-573 -0.417 99.0 0.0 0.0 noncon
hsa-miR-30b-5p 351-358 -0.416 98.0 -0.328 97.0 con
hsa-miR-30c-5p 351-358 -0.416 98.0 -0.328 97.0 con
hsa-miR-6867-5p 131-138 -0.415 95.0 -0.327 93.0 noncon
hsa-miR-4296 96-102 -0.411 98.0 -0.324 97.0 noncon
hsa-miR-4265 96-102 -0.407 98.0 -0.321 97.0 noncon
hsa-miR-22-3p 667-674 -0.403 98.0 0.0 0.0 noncon
hsa-miR-650 566-573 -0.401 98.0 0.0 0.0 noncon
hsa-miR-1184 1317-1324 -0.398 99.0 0.0 0.0 noncon
hsa-miR-4322 96-102 -0.396 98.0 -0.312 97.0 noncon
hsa-miR-4687-3p 175-182 -0.391 98.0 -0.308 97.0 noncon
hsa-miR-8081 80-87 -0.385 98.0 -0.304 97.0 noncon
hsa-miR-4455 190-196 -0.382 95.0 -0.302 92.0 noncon
hsa-miR-4308 1429-1436 -0.373 98.0 0.0 0.0 noncon
hsa-miR-6829-3p 470-477 -0.357 98.0 -0.282 98.0 noncon
hsa-miR-6791-3p 470-477 -0.349 98.0 -0.276 97.0 noncon
hsa-miR-544b 1019-1026 -0.348 98.0 0.0 0.0 noncon
hsa-miR-3121-5p 507-513 -0.346 98.0 -0.274 97.0 noncon
hsa-miR-324-5p 1246-1252 -0.339 94.0 0.0 0.0 noncon
hsa-miR-5000-3p 1475-1482 -0.337 97.0 0.0 0.0 noncon
hsa-miR-146b-3p 800-807 -0.334 98.0 0.0 0.0 noncon
hsa-miR-6747-5p 191-197 -0.332 97.0 -0.263 95.0 noncon
hsa-miR-6801-5p 222-229 -0.329 97.0 -0.261 96.0 noncon
hsa-miR-4443 567-573 -0.328 96.0 0.0 0.0 noncon
hsa-miR-3675-5p 447-453 -0.327 95.0 -0.259 94.0 noncon
hsa-miR-5004-5p 258-264 -0.321 95.0 -0.255 94.0 noncon
hsa-miR-3184-5p 233-239 -0.32 94.0 -0.254 92.0 noncon
hsa-miR-6881-3p 1379-1386 -0.319 97.0 0.0 0.0 noncon
hsa-miR-223-5p 186-193 -0.319 96.0 -0.253 96.0 noncon
hsa-miR-6880-5p 288-294 -0.317 94.0 -0.252 91.0 noncon
hsa-miR-627-5p 79-86 -0.315 98.0 -0.25 97.0 noncon
hsa-miR-1269b 73-79 -0.314 96.0 -0.314 97.0 noncon
hsa-miR-7113-3p 1431-1438 -0.312 98.0 0.0 0.0 noncon
hsa-miR-4798-3p 520-526 -0.312 98.0 -0.248 97.0 noncon
hsa-miR-423-5p 233-239 -0.309 93.0 -0.245 91.0 noncon
hsa-miR-555 277-283 -0.308 94.0 -0.244 92.0 noncon
hsa-miR-5001-3p 787-794 -0.304 96.0 0.0 0.0 noncon
hsa-miR-6867-3p 1432-1439 -0.304 96.0 0.0 0.0 noncon
hsa-miR-1269a 73-79 -0.303 95.0 -0.303 97.0 noncon
hsa-miR-27a-5p 94-100 -0.301 95.0 -0.239 93.0 noncon
hsa-miR-603 1400-1407 -0.294 99.0 0.0 0.0 noncon
hsa-miR-197-3p 29-35 -0.291 97.0 -0.291 98.0 noncon
hsa-miR-6760-3p 1558-1564 -0.29 97.0 0.0 0.0 noncon
hsa-miR-6773-3p 1080-1087 -0.289 96.0 0.0 0.0 noncon
hsa-miR-6843-3p 927-934 -0.288 96.0 0.0 0.0 noncon
hsa-miR-3690 755-762 -0.288 97.0 0.0 0.0 noncon
hsa-miR-605-3p 248-254 -0.286 94.0 -0.227 92.0 noncon
hsa-miR-5004-5p 599-606 -0.285 93.0 0.0 0.0 noncon
hsa-miR-140-3p.1 1252-1259 -0.283 97.0 0.0 0.0 noncon
hsa-miR-3158-5p 1504-1511 -0.277 97.0 0.0 0.0 noncon
hsa-miR-6836-3p 471-477 -0.276 95.0 -0.22 94.0 noncon
hsa-miR-331-5p 35-41 -0.274 94.0 -0.274 96.0 noncon
hsa-miR-3650 189-195 -0.274 94.0 -0.218 94.0 noncon
hsa-miR-4740-5p 73-79 -0.274 94.0 -0.274 95.0 noncon
hsa-miR-6738-3p 788-795 -0.273 95.0 0.0 0.0 noncon
hsa-miR-361-3p 713-720 -0.272 95.0 0.0 0.0 noncon
hsa-miR-6808-5p 293-299 -0.27 97.0 -0.215 96.0 noncon
hsa-miR-6893-5p 293-299 -0.27 97.0 -0.215 96.0 noncon
hsa-miR-7847-3p 287-293 -0.268 92.0 -0.213 89.0 noncon
hsa-miR-6818-5p 128-134 -0.267 98.0 -0.213 97.0 noncon
hsa-miR-124-5p 841-848 -0.267 93.0 0.0 0.0 noncon
hsa-miR-3065-3p 941-948 -0.267 96.0 0.0 0.0 noncon
hsa-miR-655-3p 524-530 -0.261 96.0 -0.208 95.0 con
hsa-miR-4436a 1475-1482 -0.261 97.0 0.0 0.0 noncon
hsa-miR-4736 292-298 -0.26 96.0 -0.207 95.0 noncon
hsa-miR-431-5p 207-213 -0.26 94.0 -0.207 93.0 con
hsa-miR-27a-5p 1538-1544 -0.26 92.0 0.0 0.0 noncon
hsa-miR-1197 1474-1480 -0.257 96.0 0.0 0.0 noncon
hsa-miR-4507 340-346 -0.257 94.0 -0.205 93.0 noncon
hsa-miR-5193 469-475 -0.252 92.0 -0.201 91.0 noncon