EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
CHST6



General Information
Gene Symbol:   
CHST6
Protein name:   
Carbohydrate sulfotransferase 6
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Five GlcNAc6ST enzymes have been found in humans.
• GlcNAc6ST-1 (CHST2) catalyzes the formation of 6-sulfo sialyl Lewis-X (6-sulfo sLeX, the major L-selectin ligand on HEV. This is also a major enzyme facilitating C6-GlcNAc sulfation of KS. CHST2 acts at the non-reducing end of GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3).
• GlcNAc6ST-2 (CHST4) is expressed on High Endothelial Cells of the lymph node (HEV). Both CHST2 and CHST4 can make 6-sulfo sialyl Lewis-X. It is also involved in the biosynthesis of MECA-79 epitope. CHST4 acts internally on LacNAc substrate.
• GlcNAc6ST-3 (CHST5) is a GlcNAc6ST that is functional in the intestines. It is highly expressed in colon and small intestine. CHST5 does not act to form KS, but it may attach sulfate to C6 on O-glycan core-2 glycan at the non-reducing end. As it is located in the intestines, it does not participate in the formation of the sulfated sLeX epitope.
• GlcNAc6ST-4/GST-5 (CHST7) is widely expressed in many organs. Less is known about this enzyme. It may contribute to C6 sulfation of GalNAc during chondroitin sulfate biosynthesis.
• GlcNAc6ST-5 (CHST6) is expressed in the cornea, and involved in the sulfation of keratin sulfate GAGs of the eye. The absence of this enzyme results in macular corneal dystrophy.

Related Pathway
KEGG ORTHOLOGY:   
K09671
KEGG Pathway:   
ko00533 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9GZX3 
NCBI GeneID:   
4166
GlyMap GeneID:   
4166
DNA aRefSeq:   
NM_021615.4 
Protein RefSeq:   
NP_067628.1 
BRENDA:   
Kinetic info.
OMIM number:   
605294
GeneCards database:   
CHST6
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-3)Gal(b1-?) or Gal(b1-3)[GlcNAc(b1-6)]GalNAc(a1-?)Ser|Thr or Gal(b1-3)[Gal(b1-4)GlcNAc(b1-6)]GalNAc(a1-?)Ser|Thr
Product:  
     Gal(b1-4)GlcNAc(b1-3 [6S])Gal(b1-?) or Gal(b1-3)[GlcNAc(b1-6 [6S])]GalNAc(a1-?)Ser|Thr or Gal(b1-3)[Gal(b1-4)GlcNAc(b1-6 [6S])]GalNAc(a1-?)Ser|Thr
Constraints:  
     var=50%
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-3173-5p 4203-4210 -0.428 98.0 -0.362 98.0 noncon
hsa-miR-369-5p 4654-4661 -0.41 95.0 -0.347 94.0 noncon
hsa-miR-6772-3p 5484-5491 -0.396 98.0 -0.336 97.0 noncon
hsa-miR-6799-3p 4203-4210 -0.388 98.0 -0.329 97.0 noncon
hsa-miR-7155-5p 1504-1511 -0.387 98.0 -0.387 99.0 noncon
hsa-miR-6836-3p 693-700 -0.384 98.0 -0.384 99.0 noncon
hsa-miR-3173-5p 1340-1347 -0.382 98.0 -0.382 98.0 noncon
hsa-miR-6799-3p 1340-1347 -0.366 97.0 -0.366 98.0 noncon
hsa-miR-6776-3p 1561-1568 -0.344 98.0 -0.344 98.0 noncon
hsa-miR-6775-3p 2048-2055 -0.34 95.0 -0.289 94.0 noncon
hsa-miR-1271-3p 431-438 -0.328 95.0 -0.328 97.0 noncon
hsa-miR-4750-3p 565-572 -0.325 97.0 -0.325 98.0 noncon
hsa-miR-3605-3p 69-76 -0.323 91.0 -0.323 93.0 noncon
hsa-miR-6804-3p 207-214 -0.321 95.0 -0.321 97.0 noncon
hsa-miR-876-3p 3834-3841 -0.314 95.0 -0.268 95.0 noncon
hsa-miR-3914 1422-1428 -0.313 97.0 -0.313 98.0 noncon
hsa-miR-6884-5p 4795-4801 -0.312 97.0 -0.266 96.0 noncon
hsa-miR-8071 5402-5409 -0.307 96.0 -0.262 96.0 noncon
hsa-miR-1291 2048-2055 -0.306 94.0 -0.261 93.0 noncon
hsa-miR-604 5326-5333 -0.297 97.0 -0.253 96.0 noncon
hsa-miR-550a-5p 431-438 -0.296 93.0 -0.296 96.0 noncon
hsa-miR-550a-3-5p 431-438 -0.296 93.0 -0.296 96.0 noncon
hsa-miR-4425 2455-2462 -0.295 92.0 -0.252 92.0 noncon
hsa-miR-4482-5p 1894-1901 -0.292 97.0 -0.249 97.0 noncon
hsa-miR-1271-3p 1387-1394 -0.289 93.0 -0.289 95.0 noncon
hsa-miR-485-5p 4795-4801 -0.288 96.0 -0.246 96.0 noncon
hsa-miR-6511b-3p 481-488 -0.287 95.0 -0.287 96.0 noncon
hsa-miR-6511a-3p 481-488 -0.287 95.0 -0.287 96.0 noncon
hsa-miR-605-3p 5097-5104 -0.285 94.0 -0.243 94.0 noncon
hsa-miR-5586-5p 994-1000 -0.278 95.0 -0.278 96.0 noncon
hsa-miR-3130-5p 1894-1901 -0.274 97.0 -0.234 97.0 noncon
hsa-miR-193b-5p 1502-1509 -0.27 90.0 -0.27 93.0 noncon
hsa-miR-3194-3p 767-774 -0.265 95.0 -0.265 96.0 noncon
hsa-miR-550a-3-5p 1387-1394 -0.265 91.0 -0.265 94.0 noncon
hsa-miR-550a-5p 1387-1394 -0.265 91.0 -0.265 94.0 noncon
hsa-miR-3156-3p 703-710 -0.26 95.0 -0.26 96.0 noncon
hsa-miR-1915-5p 135-142 -0.259 90.0 -0.259 93.0 noncon
hsa-miR-7156-3p 2841-2848 -0.251 94.0 -0.214 93.0 noncon
hsa-miR-3622a-5p 5034-5040 -0.251 96.0 -0.214 96.0 noncon