EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GAL3ST3



General Information
Gene Symbol:   
GAL3ST3
Protein name:   
Galactose-3-O-sulfotransferase 3
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.*
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• There are 4 enzymes that perform 3-O-sulfation of Gal.
• Gal3ST1: Acts on GalCer and LacCer to create sulfated glycolipids. Catalyzes the synthesis of sulfatides and seminolpids.
• Gal3ST2: Galβ1,4GlcNAc of O- and N-glycan, can act on sulfated substrates to form bisulfated carbohydrates like 3′,6-disulfo LewisX and 3′,6'-disulfo LewisX. This can also form 3'-sulfo-Le a and 3-O-sulfo-LeX determinants and it can act on lipid substrates
• Gal3ST3 Galβ1,4GlcNAc of O- and N-glycan and Galβ1,4GalNAc of GloboH structure. Can act on sulfated substrates to form bisulfated carbohydrates
• Gal3ST4 Galβ1,4GalNAc of Globo glycolipids and glycopeptides

Related Pathway
KEGG ORTHOLOGY:   
K09676
KEGG Pathway:   
No Record 
Reactome:   
No Record
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q96A11 
NCBI GeneID:   
89792
GlyMap GeneID:   
89792
DNA aRefSeq:   
NM_033036.2 
Protein RefSeq:   
NP_149025.1 
BRENDA:   
Kinetic info.
OMIM number:   
608234
GeneCards database:   
GAL3ST3
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?) or Gal(b1-4)GlcNAc(b1-? [6S])
Product:  
     Gal(b1-4 [3S])GlcNAc(b1-?) or Gal(b1-4 [3S])GlcNAc(b1-? [6S])
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000312006 ENST00000527878 WT1 SPIB IRF4 ZNF143 KLF4 MECOM TFAP4 PPARG CTCF GATA6 GATA2 SCRT1 SCRT2 SCRT2 STAT5B
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6825-5p 446-453 -0.474 98.0 -0.474 98.0 noncon
hsa-miR-8086 1139-1146 -0.448 97.0 -0.001 18.0 noncon
hsa-miR-7-5p 430-437 -0.446 99.0 -0.446 99.0 con
hsa-miR-4314 476-483 -0.446 99.0 -0.446 99.0 noncon
hsa-miR-3692-5p 1652-1659 -0.43 99.0 -0.001 36.0 noncon
hsa-miR-134-3p 450-457 -0.423 98.0 -0.423 99.0 noncon
hsa-miR-2114-3p 361-368 -0.421 98.0 -0.421 98.0 noncon
hsa-miR-5580-5p 1108-1115 -0.41 97.0 -0.001 16.0 noncon
hsa-miR-135a-3p 415-422 -0.41 98.0 -0.41 98.0 noncon
hsa-miR-342-5p 1637-1644 -0.409 97.0 -0.001 24.0 noncon
hsa-miR-3192-5p 475-482 -0.408 99.0 -0.408 99.0 noncon
hsa-miR-6823-3p 361-368 -0.408 98.0 -0.408 98.0 noncon
hsa-miR-133a-5p 1650-1657 -0.406 98.0 -0.001 16.0 noncon
hsa-miR-3677-5p 1354-1361 -0.404 99.0 -0.001 37.0 noncon
hsa-miR-4664-5p 1637-1644 -0.399 97.0 -0.001 26.0 noncon
hsa-miR-491-5p 448-455 -0.392 98.0 -0.392 98.0 noncon
hsa-miR-7973 1334-1341 -0.391 96.0 -0.001 25.0 noncon
hsa-miR-152-5p 479-486 -0.377 97.0 -0.377 98.0 noncon
hsa-miR-27a-5p 340-347 -0.374 98.0 -0.374 98.0 noncon
hsa-miR-4725-5p 1332-1339 -0.374 97.0 -0.001 17.0 noncon
hsa-miR-6855-3p 566-573 -0.374 98.0 -0.001 30.0 noncon
hsa-miR-4665-5p 1474-1481 -0.369 96.0 -0.001 15.0 noncon
hsa-miR-5196-5p 230-237 -0.364 98.0 -0.364 98.0 noncon
hsa-miR-6796-5p 313-320 -0.36 96.0 -0.36 97.0 noncon
hsa-miR-4756-5p 137-144 -0.36 98.0 -0.36 99.0 noncon
hsa-miR-149-3p 37-44 -0.358 97.0 -0.358 98.0 noncon
hsa-miR-4650-5p 260-267 -0.358 98.0 -0.358 98.0 noncon
hsa-miR-3679-3p 1318-1325 -0.349 95.0 -0.001 37.0 noncon
hsa-miR-4728-5p 37-44 -0.348 97.0 -0.348 98.0 noncon
hsa-miR-6827-5p 412-418 -0.345 94.0 -0.345 96.0 noncon
hsa-miR-1321 137-144 -0.344 98.0 -0.344 98.0 noncon
hsa-miR-4447 312-319 -0.343 97.0 -0.343 98.0 noncon
hsa-miR-4513 566-573 -0.342 97.0 -0.001 28.0 noncon
hsa-miR-5088-5p 86-92 -0.342 97.0 -0.342 98.0 noncon
hsa-miR-4739 137-144 -0.341 98.0 -0.341 98.0 noncon
hsa-miR-4747-5p 230-237 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-6848-5p 310-317 -0.33 91.0 -0.33 93.0 noncon
hsa-miR-6846-5p 310-317 -0.33 91.0 -0.33 93.0 noncon
hsa-miR-4446-3p 367-374 -0.327 97.0 -0.327 97.0 noncon
hsa-miR-4472 312-319 -0.327 97.0 -0.327 98.0 noncon
hsa-miR-4728-5p 396-403 -0.326 97.0 -0.326 97.0 noncon
hsa-miR-6772-3p 883-890 -0.326 96.0 -0.001 22.0 noncon
hsa-miR-7705 1179-1186 -0.322 97.0 -0.001 30.0 noncon
hsa-miR-149-3p 396-403 -0.32 97.0 -0.32 97.0 noncon
hsa-miR-3652 49-56 -0.319 98.0 -0.319 98.0 noncon
hsa-miR-6883-5p 37-44 -0.318 97.0 -0.318 97.0 noncon
hsa-miR-4722-3p 1590-1597 -0.315 98.0 -0.001 19.0 noncon
hsa-miR-6849-3p 201-208 -0.313 98.0 -0.313 98.0 noncon
hsa-miR-6887-3p 916-922 -0.313 92.0 -0.001 26.0 noncon
hsa-miR-6883-5p 396-403 -0.312 96.0 -0.312 97.0 noncon
hsa-miR-6765-3p 1119-1126 -0.308 98.0 -0.001 41.0 noncon
hsa-miR-4430 49-56 -0.308 98.0 -0.308 98.0 noncon
hsa-miR-6502-3p 1226-1233 -0.305 98.0 -0.001 37.0 noncon
hsa-miR-1275 1474-1481 -0.304 96.0 -0.001 20.0 noncon
hsa-miR-6785-5p 396-403 -0.302 96.0 -0.302 97.0 noncon
hsa-miR-6785-5p 37-44 -0.3 96.0 -0.3 97.0 noncon
hsa-miR-4519 1507-1514 -0.3 97.0 -0.001 26.0 noncon
hsa-miR-4489 742-749 -0.298 96.0 -0.001 12.0 noncon
hsa-miR-6727-3p 1590-1597 -0.297 98.0 -0.001 20.0 noncon
hsa-miR-7974 1340-1347 -0.293 93.0 -0.001 18.0 noncon
hsa-miR-4792 283-289 -0.293 95.0 -0.293 96.0 noncon
hsa-miR-6759-3p 1434-1441 -0.29 95.0 -0.001 21.0 noncon
hsa-miR-6796-5p 449-455 -0.288 92.0 -0.288 94.0 noncon
hsa-miR-4763-3p 288-294 -0.288 95.0 -0.288 96.0 noncon
hsa-miR-4705 1581-1588 -0.287 97.0 -0.001 35.0 noncon
hsa-miR-4745-5p 746-753 -0.284 92.0 -0.001 21.0 noncon
hsa-miR-6746-5p 157-163 -0.282 92.0 -0.282 94.0 noncon
hsa-miR-4488 76-82 -0.279 91.0 -0.279 92.0 noncon
hsa-miR-4697-5p 76-82 -0.279 90.0 -0.279 92.0 noncon
hsa-miR-605-5p 501-507 -0.279 98.0 -0.279 98.0 noncon
hsa-miR-1207-5p 288-294 -0.276 94.0 -0.276 95.0 noncon
hsa-miR-6726-5p 466-472 -0.276 92.0 -0.276 94.0 noncon
hsa-miR-491-5p 586-593 -0.276 93.0 -0.001 10.0 noncon
hsa-miR-1237-5p 76-82 -0.275 90.0 -0.275 92.0 noncon
hsa-miR-6728-3p 1143-1150 -0.274 97.0 -0.001 35.0 noncon
hsa-miR-3064-5p 1342-1349 -0.273 96.0 -0.001 31.0 noncon
hsa-miR-3162-5p 414-420 -0.27 91.0 -0.27 94.0 noncon
hsa-miR-150-3p 1734-1740 -0.27 90.0 -0.001 13.0 noncon
hsa-miR-431-5p 887-894 -0.268 94.0 -0.001 36.0 noncon
hsa-miR-4490 1736-1742 -0.267 93.0 -0.001 13.0 noncon
hsa-miR-183-5p.1 1418-1424 -0.265 94.0 -0.001 32.0 noncon
hsa-miR-6504-5p 1342-1349 -0.265 95.0 -0.001 30.0 noncon
hsa-miR-198 1195-1202 -0.263 96.0 -0.001 21.0 noncon
hsa-miR-4638-3p 272-279 -0.26 93.0 -0.26 95.0 noncon
hsa-miR-4757-5p 258-264 -0.257 94.0 -0.257 96.0 noncon
hsa-miR-6823-3p 410-416 -0.257 91.0 -0.257 94.0 noncon
hsa-miR-6760-5p 1129-1135 -0.256 95.0 -0.001 21.0 noncon
hsa-miR-5196-3p 597-604 -0.255 95.0 -0.001 40.0 noncon
hsa-miR-3126-5p 112-118 -0.255 94.0 -0.255 96.0 noncon
hsa-miR-4660 972-978 -0.254 93.0 -0.001 27.0 noncon
hsa-miR-3151-5p 744-750 -0.253 94.0 -0.001 33.0 noncon
hsa-miR-6720-5p 202-208 -0.252 95.0 -0.252 96.0 noncon
hsa-miR-1286 71-77 -0.251 95.0 -0.251 96.0 noncon