EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS3ST5



General Information
Gene Symbol:   
HS3ST5
Protein name:   
Heparan sulfate glucosamine 3-O-sulfotransferase 5
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.23
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Seven isoenzymes display HS 3-O-sulfotransferase activity, with all enzymes transferring sulfate to C3 of GlcNAc in heparin and heparan sulfate.
• HS3ST1 sulfates an N-sulfo glucosamine (GlcNS) that has a nonsulfated glucuronic acid (GlcA) or an iduronic acid (IdoA) at the nonreducing end. This modification is necessary for anti-thrombin activity of heparin. HS
• HS3ST-2, 3 (3A and 3B), 4, and 6, all sulfate a GlcNS6S residue that has a 2-O-sulfated iduronic acid (IdoA2S) on the nonreducing end. This does not provide anti-thrombin activity, but serves viral entry for herpes simplex virus type-1.
• The tissue distribution of the different HS3STs is quite different.

Related Pathway
KEGG ORTHOLOGY:   
K08104
KEGG Pathway:   
ko00534 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8IZT8 
NCBI GeneID:   
222537
GlyMap GeneID:   
222537
DNA aRefSeq:   
NM_153612.3 
Protein RefSeq:   
NP_705840.2 
BRENDA:   
Kinetic info.
OMIM number:   
609407
GeneCards database:   
HS3ST5
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4)GlcN(a1-4 [2S]) or IdoA(a1-4)GlcN(a1-4 [2,6S]) or GlcA(a1-4)GlcN(a1-4 [2S]) or GlcA(a1-4)GlcN(a1-4 [2,6S])
Product:  
     IdoA(a1-4)GlcN(a1-4 [2,3S]) or IdoA(a1-4)GlcN(a1-4 [2,3,6S]) or GlcA(a1-4)GlcN(a1-4 [2,3S]) or GlcA(a1-4)GlcN(a1-4 [2,3,6S])
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-3135a 77-84 -0.598 99.0 -0.598 99.0 noncon
hsa-miR-593-5p 450-457 -0.588 99.0 -0.588 99.0 noncon
hsa-miR-936 159-166 -0.485 99.0 -0.485 99.0 noncon
hsa-miR-5587-5p 132-139 -0.478 99.0 -0.478 99.0 noncon
hsa-miR-3679-3p 248-255 -0.477 98.0 -0.477 98.0 noncon
hsa-miR-4802-3p 104-111 -0.464 99.0 -0.464 99.0 noncon
hsa-miR-130b-3p 545-552 -0.451 99.0 -0.451 99.0 con
hsa-miR-130a-3p 545-552 -0.451 99.0 -0.451 99.0 con
hsa-miR-4295 545-552 -0.451 99.0 -0.451 99.0 con
hsa-miR-222-5p 160-167 -0.447 99.0 -0.447 99.0 noncon
hsa-miR-301b-3p 545-552 -0.443 99.0 -0.443 99.0 con
hsa-miR-301a-3p 545-552 -0.443 99.0 -0.443 99.0 con
hsa-miR-150-3p 354-361 -0.438 97.0 -0.438 98.0 noncon
hsa-miR-3666 545-552 -0.435 99.0 -0.435 99.0 con
hsa-miR-377-3p 344-351 -0.421 98.0 -0.421 99.0 con
hsa-miR-7112-3p 394-401 -0.415 98.0 -0.415 98.0 noncon
hsa-miR-454-3p 545-552 -0.414 99.0 -0.414 99.0 con
hsa-miR-3166 192-199 -0.4 97.0 -0.4 98.0 noncon
hsa-miR-3656 307-313 -0.399 96.0 -0.399 97.0 noncon
hsa-miR-6817-3p 643-650 -0.384 99.0 -0.384 99.0 noncon
hsa-miR-6895-3p 1082-1089 -0.366 98.0 -0.124 89.0 noncon
hsa-miR-3191-5p 1337-1344 -0.357 98.0 -0.121 87.0 noncon
hsa-miR-4522 1149-1156 -0.355 98.0 -0.12 83.0 noncon
hsa-miR-532-5p 1110-1117 -0.352 98.0 -0.119 90.0 noncon
hsa-miR-519d-3p 69-75 -0.344 98.0 -0.344 99.0 con
hsa-miR-671-5p 846-853 -0.342 97.0 -0.116 83.0 noncon
hsa-miR-6068 1058-1064 -0.341 91.0 -0.116 67.0 noncon
hsa-miR-518c-5p 1336-1343 -0.338 98.0 -0.115 87.0 noncon
hsa-miR-4524b-3p 1608-1615 -0.337 99.0 -0.115 94.0 noncon
hsa-miR-5187-3p 293-300 -0.334 98.0 -0.334 99.0 noncon
hsa-miR-1908-5p 309-315 -0.332 90.0 -0.332 92.0 noncon
hsa-miR-3180-5p 983-990 -0.329 98.0 -0.112 89.0 noncon
hsa-miR-6501-3p 1270-1277 -0.328 98.0 -0.112 88.0 noncon
hsa-miR-519b-3p 68-74 -0.326 98.0 -0.326 98.0 noncon
hsa-miR-519c-3p 68-74 -0.326 98.0 -0.326 98.0 noncon
hsa-miR-519a-3p 68-74 -0.326 98.0 -0.326 98.0 noncon
hsa-miR-20a-5p 69-75 -0.325 98.0 -0.325 99.0 con
hsa-miR-6134 1004-1011 -0.32 96.0 -0.109 76.0 noncon
hsa-miR-526b-3p 69-75 -0.318 99.0 -0.318 99.0 con
hsa-miR-3529-3p 673-680 -0.317 99.0 -0.317 99.0 noncon
hsa-miR-532-3p 1486-1493 -0.317 98.0 -0.108 91.0 noncon
hsa-miR-4684-5p 1162-1169 -0.314 98.0 -0.108 89.0 noncon
hsa-miR-208a-3p 497-503 -0.312 98.0 -0.312 99.0 noncon
hsa-miR-20b-5p 69-75 -0.311 98.0 -0.311 98.0 con
hsa-miR-6805-5p 836-842 -0.308 90.0 -0.106 74.0 noncon
hsa-miR-942-5p 228-235 -0.308 97.0 -0.308 98.0 noncon
hsa-miR-7110-3p 1499-1505 -0.303 93.0 -0.104 80.0 noncon
hsa-miR-8085 1240-1246 -0.303 95.0 -0.104 75.0 noncon
hsa-miR-208b-3p 497-503 -0.301 98.0 -0.301 99.0 noncon
hsa-miR-197-3p 536-542 -0.301 97.0 -0.301 98.0 noncon
hsa-miR-196a-3p 1296-1303 -0.3 95.0 -0.103 75.0 noncon
hsa-miR-593-5p 1358-1364 -0.3 92.0 -0.103 71.0 noncon
hsa-miR-4503 1391-1398 -0.299 96.0 -0.103 80.0 noncon
hsa-miR-7-5p 283-289 -0.299 97.0 -0.299 97.0 con
hsa-miR-6815-5p 1003-1009 -0.298 96.0 -0.102 86.0 noncon
hsa-miR-6865-5p 1003-1009 -0.298 96.0 -0.102 86.0 noncon
hsa-miR-4441 1242-1248 -0.297 97.0 -0.102 82.0 noncon
hsa-miR-1267 639-646 -0.296 97.0 -0.296 98.0 noncon
hsa-miR-5195-5p 1420-1426 -0.294 95.0 -0.101 81.0 noncon
hsa-miR-106a-5p 69-75 -0.293 98.0 -0.293 98.0 con
hsa-miR-106b-5p 69-75 -0.292 98.0 -0.292 98.0 con
hsa-miR-5197-5p 1299-1306 -0.29 96.0 -0.1 81.0 noncon
hsa-miR-6079 847-854 -0.29 97.0 -0.1 89.0 noncon
hsa-miR-32-5p 798-804 -0.289 95.0 -0.1 87.0 noncon
hsa-miR-17-5p 69-75 -0.289 97.0 -0.289 98.0 con
hsa-miR-93-5p 69-75 -0.289 98.0 -0.289 98.0 con
hsa-miR-30d-3p 162-169 -0.288 98.0 -0.288 98.0 noncon
hsa-miR-6858-3p 423-429 -0.287 97.0 -0.287 98.0 noncon
hsa-miR-6873-3p 644-651 -0.282 96.0 -0.282 97.0 noncon
hsa-miR-1304-3p 1521-1528 -0.282 98.0 -0.097 91.0 noncon
hsa-miR-345-5p 1151-1158 -0.281 98.0 -0.097 91.0 noncon
hsa-miR-30a-3p 162-169 -0.28 98.0 -0.28 98.0 noncon
hsa-miR-30e-3p 162-169 -0.28 98.0 -0.28 98.0 noncon
hsa-miR-3622a-5p 1529-1535 -0.277 97.0 -0.096 87.0 noncon
hsa-miR-183-5p.1 452-458 -0.277 95.0 -0.277 96.0 noncon
hsa-miR-4645-5p 30-37 -0.276 97.0 -0.276 98.0 noncon
hsa-miR-4270 1242-1248 -0.274 96.0 -0.095 80.0 noncon
hsa-miR-3194-3p 82-88 -0.273 96.0 -0.273 97.0 noncon
hsa-miR-6731-5p 1240-1246 -0.27 93.0 -0.093 74.0 noncon
hsa-miR-19a-3p 544-550 -0.269 92.0 -0.269 94.0 con
hsa-miR-19b-3p 544-550 -0.269 92.0 -0.269 94.0 con
hsa-miR-367-3p 798-804 -0.268 95.0 -0.093 85.0 noncon
hsa-miR-4768-5p 645-652 -0.267 96.0 -0.267 96.0 noncon
hsa-miR-6754-5p 1242-1248 -0.26 96.0 -0.09 80.0 noncon
hsa-miR-4679 1468-1474 -0.254 97.0 -0.088 87.0 noncon
hsa-miR-6818-3p 1083-1090 -0.252 96.0 -0.087 84.0 noncon
hsa-miR-7151-5p 1446-1452 -0.252 97.0 -0.087 91.0 noncon