EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
HS6ST3



General Information
Gene Symbol:   
HS6ST3
Protein name:   
Heparan-sulfate 6-O-sulfotransferase 3
Enzyme Class:   
Sulfate synthase
CAZy Family:   
E.C. No:   
2.8.2.30
Organs:   
Reaction (SNFG):   
PAPS +
→
+ PAP
PAPS +
→
+ PAP
Reaction (IUBMB):   
No specified reaction.
About

• Sulfation of heparan sulfate occurs at C2 of HexA. It also occurs at C3, C6, and NS residues of GlcNAc or GlcNS. HSPGs are major regulators of cell behavior with roles in inflammation, blood coagulation, tumor invasion, and malignancy.
• Three heparan sulfate 6-O-sulfotransferases exist that transfer sulfate to the C6 position of GlcNAc or GlcNS in heparan sulfate/heparin.
• HS6ST1 prefers iduronic acid residues adjacent to the GlcNS. HS6ST2 prefers GlcA when substrate conc is high, but it prefers IdoA at low substrate concentration. HS6ST-3 preference is intermediate between HS6ST1 and HS6ST2. These enzymes do not function when IdoA is 2-sulfated.
• HS6ST1 is ubiquitously expressed. The expression of other isoenzymes is restricted. HS6ST2 is expressed primarily in bladder, brain, testis, and thyroid. HS6ST3 slightly in brain, connective tissue, and kidney.
• HS6ST1 deficient mice display growth retardation and fatality after E15.5. H6ST1 is also associated with human disease.
• These enzymes do not act on CS/DS/KS.

Related Pathway
KEGG ORTHOLOGY:   
K08103
KEGG Pathway:   
ko00534 
Reactome:   
R-HSA-2022928
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8IZP7 
NCBI GeneID:   
266722
GlyMap GeneID:   
266722
DNA aRefSeq:   
NM_153456.3 
Protein RefSeq:   
NP_703157.2 
BRENDA:   
Kinetic info.
OMIM number:   
609401
GeneCards database:   
HS6ST3
Enzyme Specificity
Glycan Type:  
     GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     IdoA(a1-4)GlcN(a1-4 [2S]) or GlcA(a1-4)GlcN(a1-4 [2S])
Product:  
     IdoA(a1-4)GlcN(a1-4 [2,6S]) or GlcA(a1-4)GlcN(a1-4 [2,6S])
Constraints:  
     ! < IdoA(a1-4 [2S])>
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000376705 MAX ZBTB14 TCF7L1 MYCN CTCF ZBTB14 MYCN MYC MAX RUNX1 ERG TCF7L1 EGR3 RUNX1 CTCFL SMAD2 ZBTB14 ZBTB14 NRF1 RUNX1 ZBTB26 TAF1 TBP MXI1 TFAP2C ZFP64 OSR2 TFAP4 ZNF600 JUND YY1 SPI1 ERG UBTF HSF1 SP2 EGR2 SP3 KLF15 KLF15 EGR3 MAZ GLIS2 ZNF467 ZNF770 GLIS1 GLIS1 PATZ1 SP1 TFAP4 ZNF263 ZNF263 FLI1 ZNF444 ZBTB20
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-3913-3p 6350-6357 -0.401 98.0 -0.397 98.0 noncon
hsa-miR-3938 1348-1355 -0.373 98.0 -0.369 98.0 noncon
hsa-miR-4751 4130-4137 -0.338 96.0 -0.335 97.0 noncon
hsa-miR-138-5p 5669-5676 -0.334 94.0 -0.331 95.0 con
hsa-miR-4259 1313-1320 -0.311 95.0 -0.308 97.0 noncon
hsa-miR-4697-5p 4632-4639 -0.309 93.0 -0.306 94.0 noncon
hsa-miR-218-5p 6310-6317 -0.299 95.0 -0.296 96.0 con
hsa-miR-1237-5p 4632-4639 -0.296 93.0 -0.293 94.0 noncon
hsa-miR-4488 4632-4639 -0.293 92.0 -0.29 93.0 noncon
hsa-miR-1915-5p 2523-2530 -0.289 92.0 -0.286 95.0 noncon
hsa-miR-6774-5p 2985-2992 -0.285 95.0 -0.282 96.0 noncon
hsa-miR-635 2985-2992 -0.285 95.0 -0.282 96.0 noncon
hsa-miR-4540 3158-3165 -0.285 93.0 -0.282 95.0 noncon
hsa-miR-7150 1962-1969 -0.281 95.0 -0.278 96.0 noncon
hsa-miR-7847-3p 1859-1866 -0.269 92.0 -0.266 94.0 noncon
hsa-miR-877-3p 5111-5118 -0.268 94.0 -0.266 95.0 noncon
hsa-miR-1248 1363-1370 -0.258 96.0 -0.256 97.0 noncon
hsa-miR-4755-3p 5622-5629 -0.257 92.0 -0.255 95.0 noncon
hsa-miR-219b-5p 118-124 -0.254 90.0 -0.254 93.0 noncon
hsa-miR-766-3p 155-162 -0.251 96.0 -0.251 97.0 noncon