EnzFunc

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
LARGE1



General Information
Gene Symbol:   
LARGE1
Protein name:   
LARGE xylosyl- and glucuronyltransferase 1
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT49
E.C. No:   
2.4.2.26
Organs:   
Reaction (SNFG):   
+
→
+ UDP
+
→
+ UDP
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• LARGE1 (formerly LARGE) and its paralog LARGE2 (formerly GYLTL1B) catalyze the formation of glycosaminoglycan (GAG)-like repeating disaccharide units ([-Xyl-α3-GlcA-β3-]n) in the Golgi
• These bifunctional enzymes polymerize and extend O-Man glycans of dystroglycan (DAG1)

Related Pathway
KEGG ORTHOLOGY:   
K09668
KEGG Pathway:   
ko00515  ko01100 
Reactome:   
R-HSA-5083627
Related Database
Organism:   
Homo sapiens
Uniprot:   
O95461 
NCBI GeneID:   
9215
GlyMap GeneID:   
9215
DNA aRefSeq:   
NM_004737.5 
Protein RefSeq:   
NP_004728.1 
BRENDA:   
Kinetic info.
OMIM number:   
603590
GeneCards database:   
LARGE1
Enzyme Specificity
Glycan Type:  
     O-linked (other)
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcA(b1-4)Xyl(b1-4) or GlcA(b1-3) or Xyl(a1-?)
Product:  
     Xyl(a1-3)GlcA(b1-4)Xyl(b1-4) or Xyl(a1-3)GlcA(b1-3) or GlcA(b1-3)Xyl(a1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells
No expression data available.
Transcription factor {ref. 10}
No high degree of confidence data