FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
GLB1



General Information
Gene Symbol:   
GLB1
Protein name:   
Beta-galactosidase
Enzyme Class:   
Glycosidase
CAZy Family:   
GH35
E.C. No:   
3.2.1.23
Organs:   
Reaction (SNFG):   
GLB1 GLB1
H2O +
→
+
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Beta galactoisdase 1 removes terminal galactose residues from various contexts (glycolipids, glycoproteins, glycosaminoglycans)
• Alternative splicing of GLB1 creates an elastin binding protein important for maintaining cellular matrix integrity and lysosomal protein complex structure.
• G(M1) gangliosidosis disorder is caused by the lack of GLB1 activity.
• GLB1 forms complexes with other proteins in the lysosome, such as protective protein calthepsin A (PPCA), neuramidase 1 (NEU1), and GalNAc 6 sulfatase (GALNS).

Related Pathway
KEGG ORTHOLOGY:   
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
P16278 
NCBI GeneID:   
2720
GlyMap GeneID:   
2720
DNA aRefSeq:   
NM_000404.3 
Protein RefSeq:   
NP_000395.2 
BRENDA:   
Kinetic info.
OMIM number:   
611458
GeneCards database:   
GLB1
Enzyme Specificity
Glycan Type:  
     
Compartment:  
     
Substrate:  
     Gal(b1-?)GlcNAc(b1-?) or Gal(b1-?)GalNAc(b1-?)
Product:  
     GlcNAc(b1-?) or GalNAc(b1-?)
Constraints:  
     None
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-5685 387-394 -0.599 99.0 -0.082 82.0 noncon
hsa-miR-554 493-500 -0.595 99.0 -0.001 35.0 noncon
hsa-miR-4999-3p 252-259 -0.565 99.0 -0.565 99.0 noncon
hsa-miR-4795-5p 400-407 -0.526 99.0 -0.074 75.0 noncon
hsa-miR-1294 42-49 -0.52 99.0 -0.52 99.0 noncon
hsa-miR-4690-3p 387-394 -0.513 99.0 -0.072 86.0 noncon
hsa-miR-3913-3p 318-325 -0.491 99.0 -0.07 63.0 noncon
hsa-miR-8055 516-523 -0.484 99.0 -0.001 53.0 noncon
hsa-miR-202-3p 40-47 -0.457 98.0 -0.457 99.0 noncon
hsa-miR-125a-3p 121-128 -0.446 99.0 -0.446 99.0 noncon
hsa-miR-6512-3p 228-235 -0.423 98.0 -0.423 99.0 noncon
hsa-miR-154-5p 500-507 -0.417 99.0 -0.001 27.0 noncon
hsa-miR-6720-5p 228-235 -0.415 98.0 -0.415 99.0 noncon
hsa-miR-4999-5p 511-517 -0.414 97.0 -0.001 18.0 noncon
hsa-miR-183-5p.2 148-155 -0.41 98.0 -0.41 98.0 noncon
hsa-miR-4677-3p 44-51 -0.388 99.0 -0.388 99.0 noncon
hsa-miR-3606-5p 404-411 -0.382 98.0 -0.056 64.0 noncon
hsa-miR-6799-3p 224-230 -0.382 98.0 -0.382 98.0 noncon
hsa-miR-5009-3p 104-111 -0.356 99.0 -0.356 99.0 noncon
hsa-miR-9-3p 240-247 -0.356 98.0 -0.356 99.0 noncon
hsa-miR-2052 518-525 -0.352 99.0 -0.001 25.0 noncon
hsa-miR-1271-5p 149-156 -0.35 96.0 -0.35 97.0 noncon
hsa-miR-3160-3p 514-520 -0.335 98.0 -0.001 19.0 noncon
hsa-miR-3692-3p 86-93 -0.332 97.0 -0.332 98.0 noncon
hsa-miR-5190 322-328 -0.328 98.0 -0.049 84.0 noncon
hsa-miR-6076 60-66 -0.328 97.0 -0.328 98.0 noncon
hsa-miR-4749-3p 188-194 -0.328 95.0 -0.328 96.0 noncon
hsa-miR-1321 53-59 -0.327 97.0 -0.327 98.0 noncon
hsa-miR-3173-5p 224-230 -0.327 96.0 -0.327 97.0 noncon
hsa-miR-2115-5p 236-242 -0.326 96.0 -0.326 97.0 noncon
hsa-miR-96-5p 149-156 -0.321 96.0 -0.321 97.0 noncon
hsa-miR-4487 514-520 -0.301 96.0 -0.001 23.0 noncon
hsa-miR-4693-5p 201-208 -0.3 97.0 -0.3 98.0 noncon
hsa-miR-6502-3p 355-361 -0.297 98.0 -0.045 79.0 noncon
hsa-miR-4489 156-162 -0.296 96.0 -0.296 97.0 noncon
hsa-miR-6797-3p 60-66 -0.294 96.0 -0.294 97.0 noncon
hsa-miR-4739 53-59 -0.287 96.0 -0.287 97.0 noncon
hsa-miR-548ba 282-288 -0.284 97.0 -0.284 98.0 noncon
hsa-miR-4276 73-79 -0.281 96.0 -0.281 97.0 noncon
hsa-miR-4270 54-60 -0.279 96.0 -0.279 97.0 noncon
hsa-miR-3151-5p 157-163 -0.272 95.0 -0.272 96.0 noncon
hsa-miR-4441 54-60 -0.269 96.0 -0.269 97.0 noncon
hsa-miR-5685 191-197 -0.267 96.0 -0.267 97.0 noncon
hsa-miR-6838-5p 459-465 -0.266 93.0 -0.001 20.0 noncon
hsa-miR-6715a-3p 442-449 -0.263 96.0 -0.001 23.0 noncon
hsa-miR-6755-5p 29-35 -0.263 90.0 -0.263 92.0 noncon
hsa-miR-4756-5p 53-59 -0.261 95.0 -0.261 96.0 noncon
hsa-miR-3688-5p 64-70 -0.255 93.0 -0.255 95.0 noncon
hsa-miR-7106-5p 52-58 -0.255 96.0 -0.255 97.0 noncon
hsa-miR-15b-5p 459-465 -0.254 92.0 -0.001 20.0 noncon
hsa-miR-16-5p 459-465 -0.254 92.0 -0.001 23.0 noncon
hsa-miR-195-5p 459-465 -0.254 92.0 -0.001 23.0 noncon
hsa-miR-675-3p 212-218 -0.253 95.0 -0.253 96.0 noncon