FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
NEU1



General Information
Gene Symbol:   
NEU1
Protein name:   
Sialidase-1
Enzyme Class:   
Glycosidase
CAZy Family:   
GH33
E.C. No:   
3.2.1.18
Organs:   
Reaction (SNFG):   
NEU1 NEU1
H2O +
→
+
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Removes α2,3 and α2,6 sialic acid (N-acetylneuraminic acid) from glycoproteins and glycolipids in lysosomal compartments.
• Lower activity toward α2,8 sialic acid.
• During N-glycan degradation, oligosaccharides sialidase removal typically precedes activity of α- and β-Galactosidase, β-N-Acetylhexosaminidases, and α-Mannosidases. Manβ1-4GlcNAc is cleaved by β-Mannosidase in the last step.

Related Pathway
KEGG ORTHOLOGY:   
K01186
KEGG Pathway:   
ko00511  ko00600  ko04142 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q99519 
NCBI GeneID:   
4758
GlyMap GeneID:   
4758
DNA aRefSeq:   
NM_000434.3 
Protein RefSeq:   
NP_000425.1 
BRENDA:   
Kinetic info.
OMIM number:   
608272
GeneCards database:   
NEU1
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GSL
Compartment:  
     
Substrate:  
     Neu5Ac(a2-?)Gal(b1-?) or Neu5Ac(a2-?)Neu5Ac(a2-?)
Product:  
     Gal(b1-?) or Neu5Ac(a2-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 50 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-125a-5p 332-339 -0.622 99.0 -0.622 99.0 con
hsa-miR-4319 332-339 -0.622 99.0 -0.622 99.0 con
hsa-miR-205-5p 497-504 -0.618 99.0 -0.617 99.0 con
hsa-miR-125b-5p 332-339 -0.614 99.0 -0.614 99.0 con
hsa-miR-4449 268-275 -0.611 98.0 -0.611 98.0 noncon
hsa-miR-1910-5p 163-170 -0.536 99.0 -0.536 99.0 noncon
hsa-miR-4652-5p 401-408 -0.522 98.0 -0.521 99.0 noncon
hsa-miR-6778-5p 185-192 -0.522 99.0 -0.522 99.0 noncon
hsa-miR-1226-3p 392-399 -0.514 99.0 -0.513 99.0 noncon
hsa-miR-1233-5p 185-192 -0.506 99.0 -0.506 99.0 noncon
hsa-miR-1247-3p 266-273 -0.504 99.0 -0.504 99.0 noncon
hsa-miR-4294 109-116 -0.471 99.0 -0.471 99.0 noncon
hsa-miR-3616-3p 202-209 -0.467 98.0 -0.467 98.0 noncon
hsa-miR-6762-5p 243-250 -0.466 97.0 -0.466 98.0 noncon
hsa-miR-6845-5p 243-250 -0.45 97.0 -0.45 98.0 noncon
hsa-miR-4509 104-111 -0.428 99.0 -0.428 99.0 noncon
hsa-miR-4722-3p 411-418 -0.421 99.0 -0.42 99.0 noncon
hsa-miR-4714-5p 428-435 -0.412 99.0 -0.411 99.0 noncon
hsa-miR-6727-3p 411-418 -0.403 99.0 -0.402 99.0 noncon
hsa-miR-664a-3p 506-513 -0.402 99.0 -0.401 99.0 noncon
hsa-miR-605-3p 27-34 -0.388 98.0 -0.388 98.0 noncon
hsa-miR-4667-5p 403-410 -0.388 98.0 -0.387 98.0 noncon
hsa-miR-4700-5p 403-410 -0.386 98.0 -0.385 99.0 noncon
hsa-miR-513a-5p 621-628 -0.376 98.0 -0.001 38.0 noncon
hsa-miR-8089 403-410 -0.375 98.0 -0.375 98.0 noncon
hsa-miR-8085 183-190 -0.368 97.0 -0.368 98.0 noncon
hsa-miR-6731-5p 183-190 -0.368 97.0 -0.368 98.0 noncon
hsa-miR-3186-5p 79-85 -0.367 97.0 -0.367 98.0 noncon
hsa-miR-1283 514-521 -0.36 99.0 -0.012 41.0 noncon
hsa-miR-4457 573-580 -0.352 98.0 -0.001 36.0 noncon
hsa-miR-6721-5p 200-206 -0.346 96.0 -0.346 97.0 noncon
hsa-miR-1286 198-204 -0.345 98.0 -0.345 98.0 noncon
hsa-miR-4330 209-216 -0.334 98.0 -0.334 99.0 noncon
hsa-miR-670-5p 333-340 -0.327 99.0 -0.327 99.0 noncon
hsa-miR-605-3p 41-48 -0.326 96.0 -0.326 97.0 noncon
hsa-miR-4747-5p 132-138 -0.325 97.0 -0.325 97.0 noncon
hsa-miR-3937 81-87 -0.322 95.0 -0.322 97.0 noncon
hsa-miR-766-3p 73-80 -0.32 98.0 -0.32 98.0 noncon
hsa-miR-521 611-617 -0.319 93.0 -0.001 12.0 noncon
hsa-miR-2355-5p 341-348 -0.319 97.0 -0.319 98.0 noncon
hsa-miR-4692 618-624 -0.315 98.0 -0.001 36.0 noncon
hsa-miR-4309 110-116 -0.314 97.0 -0.314 97.0 noncon
hsa-miR-4530 71-78 -0.312 97.0 -0.312 98.0 noncon
hsa-miR-1226-5p 203-209 -0.31 97.0 -0.31 98.0 noncon
hsa-miR-6810-5p 402-408 -0.301 96.0 -0.301 97.0 noncon
hsa-miR-3198 110-116 -0.299 98.0 -0.299 98.0 noncon
hsa-miR-5195-5p 17-23 -0.295 96.0 -0.295 97.0 noncon
hsa-miR-5196-5p 132-138 -0.292 95.0 -0.292 96.0 noncon
hsa-miR-554 437-443 -0.291 94.0 -0.291 96.0 noncon
hsa-miR-6830-3p 86-93 -0.287 97.0 -0.287 98.0 noncon
hsa-miR-3652 113-119 -0.285 97.0 -0.285 98.0 noncon
hsa-miR-4540 436-442 -0.279 93.0 -0.279 95.0 noncon
hsa-miR-4430 113-119 -0.278 97.0 -0.278 98.0 noncon
hsa-miR-583 652-658 -0.273 98.0 -0.001 50.0 noncon
hsa-miR-500b-3p 583-590 -0.273 98.0 -0.001 44.0 noncon
hsa-miR-6784-3p 585-591 -0.271 94.0 -0.001 35.0 noncon
hsa-miR-23b-3p 324-331 -0.27 94.0 -0.27 95.0 con
hsa-miR-23c 324-331 -0.27 94.0 -0.27 95.0 con
hsa-miR-23a-3p 324-331 -0.27 94.0 -0.27 95.0 con
hsa-miR-6862-3p 585-591 -0.268 94.0 -0.001 38.0 noncon
hsa-miR-6879-5p 201-207 -0.268 94.0 -0.268 96.0 noncon
hsa-miR-128-1-5p 244-250 -0.264 92.0 -0.264 93.0 noncon
hsa-miR-9500 133-139 -0.262 97.0 -0.262 97.0 noncon
hsa-miR-4645-3p 218-224 -0.26 94.0 -0.26 96.0 noncon
hsa-miR-92a-2-5p 246-253 -0.255 94.0 -0.255 95.0 noncon
hsa-miR-2682-5p 617-623 -0.254 97.0 -0.001 42.0 noncon
hsa-miR-1245b-3p 207-214 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-2467-5p 353-359 -0.251 95.0 -0.251 96.0 noncon
hsa-miR-4514 618-624 -0.25 96.0 -0.001 35.0 noncon