FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
NEU2



General Information
Gene Symbol:   
NEU2
Protein name:   
Sialidase-2
Enzyme Class:   
Glycosidase
CAZy Family:   
GH33
E.C. No:   
3.2.1.18
Organs:   
Reaction (SNFG):   
NEU2 NEU2
H2O +
→
+
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Catalyzes cleavage of α2,3 sialic acid on various glycolipids, glycoproteins and oligosacharides in cellular cytoplasm.
• Shown to function on GM1, GD1a, GT1b ganglioside. Also cleaves sialic acid on glycoprotein and milk oligosaccharides.

Related Pathway
KEGG ORTHOLOGY:   
K12357
KEGG Pathway:   
ko00511  ko00600 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9Y3R4 
NCBI GeneID:   
4759
GlyMap GeneID:   
4759
DNA aRefSeq:   
NM_005383.2 
Protein RefSeq:   
NP_005374.2 
BRENDA:   
Kinetic info.
OMIM number:   
605528
GeneCards database:   
NEU2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-?)Gal(b1-?) or NeuAc(a2-?)NeuAc(a2-?)
Product:  
     Gal(b1-?) or NeuAc(a2-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000233840