FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
NEU4



General Information
Gene Symbol:   
NEU4
Protein name:   
Sialidase-4
Enzyme Class:   
Glycosidase
CAZy Family:   
GH33
E.C. No:   
3.2.1.18
Organs:   
Reaction (SNFG):   
NEU4 NEU4
H2O +
→
+
H2O +
→
+
Reaction (IUBMB):   
No specified reaction.
About

• Catalyzes exo-sialidase cleavage of sialic acid on various glycolipids, glycoproteins and oligosacharides.
• Shown to function on α2,3/6/8 sialic acid.
• Has functional activity toward mucin, fetuin, sialyllactose.
• During lysosomal N-glycan degradation, oligosaccharides sialidase removal typically precedes activity of α- and β-Galactosidase, β-N-Acetylhexosaminidases, and α-Mannosidases. Manβ1-4GlcNAc is cleaved by β-Mannosidase in the last step.

Related Pathway
KEGG ORTHOLOGY:   
K12357
KEGG Pathway:   
ko00511  ko00600 
Reactome:   
R-HSA-1660662
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8WWR8 
NCBI GeneID:   
129807
GlyMap GeneID:   
129807
DNA aRefSeq:   
NM_001167599.2 
Protein RefSeq:   
NP_001161071.1 
BRENDA:   
Kinetic info.
OMIM number:   
608527
GeneCards database:   
NEU4
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GSL
Compartment:  
     
Substrate:  
     NeuAc(a2-?)Gal(b1-?) or NeuAc(a2-?)NeuAc(a2-?)
Product:  
     Gal(b1-?) or NeuAc(a2-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 6 4 2 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6799-3p 187-194 -0.538 99.0 -0.538 99.0 noncon
hsa-miR-3173-5p 187-194 -0.522 99.0 -0.522 99.0 noncon
hsa-miR-140-3p.1 105-112 -0.494 99.0 -0.494 99.0 noncon
hsa-miR-6125 248-255 -0.459 98.0 -0.459 98.0 noncon
hsa-miR-324-3p 38-45 -0.43 98.0 -0.43 99.0 noncon
hsa-miR-1913 38-45 -0.412 98.0 -0.412 99.0 noncon
hsa-miR-3127-3p 162-169 -0.375 97.0 -0.375 98.0 noncon
hsa-miR-6756-3p 162-169 -0.375 97.0 -0.375 98.0 noncon
hsa-miR-6752-3p 191-197 -0.34 96.0 -0.34 97.0 noncon
hsa-miR-6775-3p 261-268 -0.333 94.0 -0.333 96.0 noncon
hsa-miR-1291 261-268 -0.307 94.0 -0.307 95.0 noncon
hsa-miR-3943 85-91 -0.303 95.0 -0.303 96.0 noncon
hsa-miR-1225-3p 86-92 -0.294 94.0 -0.294 95.0 noncon
hsa-miR-1913 200-206 -0.281 95.0 -0.281 96.0 noncon
hsa-miR-324-3p 200-206 -0.271 95.0 -0.271 96.0 noncon
hsa-miR-6793-3p 58-64 -0.266 97.0 -0.266 98.0 noncon
hsa-miR-4447 236-243 -0.266 95.0 -0.266 96.0 noncon
hsa-miR-4472 236-243 -0.266 95.0 -0.266 96.0 noncon