FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG1



General Information
Gene Symbol:   
ALG1
Protein name:   
Chitobiosyldiphosphodolichol beta-mannosyltransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT33
E.C. No:   
2.4.1.142
Organs:   
Reaction (SNFG):   
+
→
+ GDP
Reaction (IUBMB):   
No specified reaction.
About

• These enzymes sequentially add 5-mannose from GDP-man to generate Man5Glc2-PP-Dol.
• Alg1 is a β1-4Mannosyltransferase.
• Alg2 adds both a(1-3) and a(1-6) Mannose to Man1GlcNAc2-PP-Dol.
• Alg1, Alg2, and Alg11 mannosyltransferases are ER membrane proteins with catalytic domains that face the cytosol and that use GDP-man as the sugar donor for mannosylation.
• Deficiencies in Alg1, Alg2, and Alg11 result in CDG-Ik, CDG-Ii and CDG-Ip, respectively.
• Intermediate glycan built on dolichol phosphate containing 7 monosaccahrides is flipped into the ER lumen. This is further built with 4 Man and 3 Glc.

Related Pathway
KEGG ORTHOLOGY:   
K03842
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9BT22 
NCBI GeneID:   
56052
GlyMap GeneID:   
56052
DNA aRefSeq:   
NM_001330504.1 
Protein RefSeq:   
NP_001317433.1 
BRENDA:   
Kinetic info.
OMIM number:   
605907
GeneCards database:   
ALG1
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP
Product:  
     Man(b1-4)GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1911-3p 131-138 -0.517 99.0 -0.517 99.0 noncon
hsa-miR-193b-5p 19-26 -0.467 98.0 -0.467 98.0 noncon
hsa-miR-4515 655-662 -0.452 98.0 -0.226 91.0 noncon
hsa-miR-4274 2413-2420 -0.397 99.0 -0.2 95.0 noncon
hsa-miR-6852-5p 22-29 -0.396 98.0 -0.396 99.0 noncon
hsa-miR-3922-5p 174-181 -0.394 98.0 -0.206 94.0 noncon
hsa-miR-337-5p 1551-1558 -0.37 95.0 -0.187 86.0 noncon
hsa-miR-1269b 656-663 -0.367 97.0 -0.186 89.0 noncon
hsa-miR-874-3p 67-74 -0.355 98.0 -0.355 98.0 noncon
hsa-miR-149-5p 505-512 -0.353 98.0 -0.186 94.0 noncon
hsa-miR-1269a 656-663 -0.351 97.0 -0.178 89.0 noncon
hsa-miR-6783-5p 2438-2445 -0.351 98.0 -0.178 90.0 noncon
hsa-miR-6791-5p 386-393 -0.347 97.0 -0.183 90.0 noncon
hsa-miR-4313 567-574 -0.347 95.0 -0.176 84.0 noncon
hsa-miR-1258 336-343 -0.342 95.0 -0.18 84.0 noncon
hsa-miR-6746-5p 1905-1912 -0.334 96.0 -0.17 82.0 noncon
hsa-miR-8070 2345-2352 -0.33 97.0 -0.168 94.0 noncon
hsa-miR-4292 386-393 -0.323 96.0 -0.171 89.0 noncon
hsa-miR-449c-3p 2313-2320 -0.319 96.0 -0.163 90.0 noncon
hsa-miR-4455 1488-1495 -0.319 91.0 -0.163 78.0 noncon
hsa-miR-3190-5p 99-106 -0.317 98.0 -0.317 98.0 noncon
hsa-miR-19a-3p 479-486 -0.315 95.0 -0.167 85.0 noncon
hsa-miR-19b-3p 479-486 -0.315 95.0 -0.167 85.0 noncon
hsa-miR-6748-5p 1490-1497 -0.304 92.0 -0.156 77.0 noncon
hsa-miR-4734 44-50 -0.295 92.0 -0.295 94.0 noncon
hsa-miR-7976 66-73 -0.29 97.0 -0.29 98.0 noncon
hsa-miR-5189-3p 170-176 -0.288 94.0 -0.153 89.0 noncon
hsa-miR-6862-3p 756-763 -0.28 94.0 -0.144 87.0 noncon
hsa-miR-6821-5p 2292-2298 -0.275 92.0 -0.141 81.0 noncon
hsa-miR-584-3p 425-432 -0.272 95.0 -0.145 90.0 noncon
hsa-miR-6741-3p 1549-1555 -0.263 92.0 -0.136 78.0 noncon
hsa-miR-6510-5p 241-248 -0.261 95.0 -0.139 88.0 noncon
hsa-miR-6722-3p 240-246 -0.258 91.0 -0.138 80.0 noncon
hsa-miR-588 98-104 -0.257 96.0 -0.257 97.0 noncon
hsa-miR-6784-3p 756-763 -0.251 92.0 -0.13 85.0 noncon
hsa-miR-1273g-3p 120-126 -0.25 96.0 -0.25 97.0 noncon