FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
ALG14



General Information
Gene Symbol:   
ALG14
Protein name:   
UDP-N-acetylglucosamine transferase subunit ALG14 homolog
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT1
E.C. No:   
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• Alg13/Alg14 are part of a heterodimer that transfers second GlcNAc from UDP-GlcNAc to generate Dol-PP-GlcNAc2
• Cells lacking these activities grow slowly, and mutations in Alg13 leads to CDG-1r

Related Pathway
KEGG ORTHOLOGY:   
K07441
KEGG Pathway:   
ko00510  ko00513  ko01100 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q96F25 
NCBI GeneID:   
199857
GlyMap GeneID:   
199857
DNA aRefSeq:   
NM_001305242.1 
Protein RefSeq:   
NP_001292171.1 
BRENDA:   
Kinetic info.
OMIM number:   
612866
GeneCards database:   
ALG14
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     GlcNAc(a1-?)Dol-PP
Product:  
     GlcNAc(b1-4)GlcNAc(a1-?)Dol-PP
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1244 197-204 -0.657 99.0 -0.629 99.0 noncon
hsa-miR-6874-3p 303-310 -0.639 99.0 -0.388 99.0 noncon
hsa-miR-148b-5p 303-310 -0.636 99.0 -0.387 99.0 noncon
hsa-miR-3679-3p 59-66 -0.618 99.0 -0.618 99.0 noncon
hsa-miR-299-5p 220-227 -0.613 99.0 -0.581 99.0 noncon
hsa-miR-3664-3p 103-110 -0.57 99.0 -0.57 99.0 noncon
hsa-miR-4744 17-24 -0.542 99.0 -0.542 99.0 noncon
hsa-miR-3686 300-307 -0.527 99.0 -0.325 98.0 noncon
hsa-miR-6837-3p 150-157 -0.523 99.0 -0.52 99.0 noncon
hsa-miR-6762-3p 1372-1379 -0.523 99.0 -0.011 37.0 noncon
hsa-miR-8067 235-242 -0.522 99.0 -0.496 99.0 noncon
hsa-miR-125b-1-3p 7646-7653 -0.518 98.0 -0.004 19.0 noncon
hsa-miR-8068 178-185 -0.507 99.0 -0.486 99.0 noncon
hsa-miR-6720-3p 1659-1666 -0.501 96.0 -0.004 18.0 noncon
hsa-miR-3607-3p 299-306 -0.495 99.0 -0.307 99.0 noncon
hsa-miR-181b-3p 148-155 -0.482 99.0 -0.479 99.0 noncon
hsa-miR-181b-2-3p 148-155 -0.482 99.0 -0.479 99.0 noncon
hsa-miR-504-3p 537-544 -0.477 99.0 -0.01 58.0 noncon
hsa-miR-6722-5p 2500-2507 -0.477 96.0 -0.004 18.0 noncon
hsa-miR-6508-5p 235-242 -0.477 99.0 -0.453 99.0 noncon
hsa-miR-647 1373-1380 -0.473 99.0 -0.01 61.0 noncon
hsa-miR-1468-3p 25-32 -0.46 99.0 -0.46 99.0 noncon
hsa-miR-4420 148-155 -0.449 99.0 -0.446 99.0 noncon
hsa-miR-5571-5p 107-114 -0.441 99.0 -0.441 99.0 noncon
hsa-miR-5089-3p 1029-1036 -0.419 98.0 -0.009 32.0 noncon
hsa-miR-1-5p 1475-1482 -0.418 99.0 -0.009 40.0 noncon
hsa-miR-5002-5p 224-230 -0.41 99.0 -0.39 99.0 noncon
hsa-miR-4753-5p 613-620 -0.409 98.0 -0.009 32.0 noncon
hsa-miR-135b-3p 71-77 -0.4 95.0 -0.4 96.0 noncon
hsa-miR-196a-5p 257-263 -0.394 97.0 -0.375 98.0 noncon
hsa-miR-196b-5p 257-263 -0.394 97.0 -0.375 98.0 noncon
hsa-miR-487a-3p 1477-1484 -0.389 99.0 -0.008 54.0 con
hsa-miR-3199 3575-3582 -0.386 97.0 -0.003 26.0 noncon
hsa-miR-154-3p 1477-1484 -0.381 99.0 -0.008 55.0 con
hsa-miR-2114-3p 8206-8213 -0.372 97.0 -0.003 34.0 noncon
hsa-miR-644a 5938-5945 -0.37 97.0 -0.003 38.0 noncon
hsa-miR-8052 3575-3582 -0.365 97.0 -0.003 28.0 noncon
hsa-miR-181c-3p 117-123 -0.362 99.0 -0.362 99.0 noncon
hsa-miR-4705 1512-1519 -0.361 98.0 -0.005 46.0 noncon
hsa-miR-6873-5p 1345-1352 -0.361 98.0 -0.008 29.0 noncon
hsa-miR-6873-3p 402-409 -0.357 98.0 -0.008 39.0 noncon
hsa-miR-874-5p 1074-1081 -0.355 96.0 -0.008 20.0 noncon
hsa-miR-7109-3p 162-168 -0.355 98.0 -0.341 98.0 noncon
hsa-miR-4462 2814-2821 -0.352 92.0 -0.003 19.0 noncon
hsa-miR-197-5p 7563-7570 -0.348 93.0 -0.003 14.0 noncon
hsa-miR-6879-3p 912-919 -0.348 94.0 -0.008 39.0 noncon
hsa-miR-3613-3p 7054-7061 -0.346 99.0 -0.003 39.0 noncon
hsa-miR-2467-3p 1354-1360 -0.343 98.0 -0.008 40.0 noncon
hsa-miR-4638-5p 878-885 -0.34 90.0 -0.007 33.0 noncon
hsa-miR-6823-3p 8206-8213 -0.335 96.0 -0.003 32.0 noncon
hsa-miR-3664-5p 623-630 -0.334 98.0 -0.007 45.0 noncon
hsa-miR-34b-3p 1499-1506 -0.334 99.0 -0.007 50.0 noncon
hsa-miR-4290 7923-7930 -0.332 92.0 -0.003 26.0 noncon
hsa-miR-519e-3p 4688-4695 -0.331 96.0 -0.003 25.0 noncon
hsa-miR-3937 6486-6493 -0.329 96.0 -0.003 22.0 noncon
hsa-miR-3132 7563-7570 -0.327 92.0 -0.003 14.0 noncon
hsa-miR-7162-5p 500-507 -0.326 97.0 -0.007 33.0 noncon
hsa-miR-942-5p 1416-1423 -0.325 98.0 -0.007 43.0 noncon
hsa-miR-5585-5p 586-593 -0.322 97.0 -0.007 46.0 noncon
hsa-miR-3169 100-106 -0.318 97.0 -0.318 98.0 noncon
hsa-miR-33b-3p 4688-4695 -0.318 96.0 -0.002 26.0 noncon
hsa-miR-154-3p 323-329 -0.317 98.0 -0.201 96.0 con
hsa-miR-487a-3p 323-329 -0.317 98.0 -0.201 96.0 con
hsa-miR-4786-3p 520-527 -0.317 97.0 -0.007 30.0 noncon
hsa-miR-618 315-321 -0.312 99.0 -0.198 97.0 noncon
hsa-miR-493-5p 135-141 -0.31 98.0 -0.31 98.0 noncon
hsa-miR-3126-5p 3370-3377 -0.305 96.0 -0.002 34.0 noncon
hsa-miR-539-3p 322-328 -0.304 94.0 -0.193 88.0 noncon
hsa-miR-516a-3p 500-507 -0.302 96.0 -0.007 33.0 noncon
hsa-miR-516b-3p 500-507 -0.302 96.0 -0.007 33.0 noncon
hsa-miR-601 5060-5067 -0.3 91.0 -0.002 19.0 noncon
hsa-miR-1911-5p 47-53 -0.299 92.0 -0.299 95.0 noncon
hsa-miR-4728-3p 491-498 -0.299 98.0 -0.007 36.0 noncon
hsa-miR-6731-3p 1275-1282 -0.299 96.0 -0.007 49.0 noncon
hsa-miR-1306-5p 7958-7965 -0.296 96.0 -0.002 44.0 noncon
hsa-miR-504-3p 896-903 -0.292 96.0 -0.007 50.0 noncon
hsa-miR-4507 7663-7670 -0.292 95.0 -0.002 38.0 noncon
hsa-miR-301a-3p 535-541 -0.291 96.0 -0.007 44.0 con
hsa-miR-301b-3p 535-541 -0.291 96.0 -0.007 44.0 con
hsa-miR-758-3p 8331-8338 -0.291 97.0 -0.002 45.0 noncon
hsa-miR-4314 7417-7423 -0.289 95.0 -0.002 21.0 noncon
hsa-miR-5001-5p 8159-8165 -0.289 92.0 -0.002 23.0 noncon
hsa-miR-5702 144-150 -0.289 95.0 -0.289 96.0 noncon
hsa-miR-4498 8159-8165 -0.288 92.0 -0.002 23.0 noncon
hsa-miR-6766-5p 6299-6306 -0.287 91.0 -0.002 25.0 noncon
hsa-miR-6833-3p 403-410 -0.287 96.0 -0.006 54.0 noncon
hsa-miR-4443 548-554 -0.283 94.0 -0.006 42.0 noncon
hsa-miR-3688-5p 5936-5943 -0.282 95.0 -0.002 23.0 noncon
hsa-miR-6875-5p 3370-3377 -0.281 95.0 -0.002 33.0 noncon
hsa-miR-4768-5p 403-410 -0.281 96.0 -0.006 54.0 noncon
hsa-miR-1266-5p 104-110 -0.279 96.0 -0.279 97.0 noncon
hsa-miR-452-3p 140-146 -0.279 97.0 -0.279 98.0 noncon
hsa-miR-1288-3p 4538-4544 -0.278 93.0 -0.002 31.0 noncon
hsa-miR-6078 788-794 -0.275 94.0 -0.006 24.0 noncon
hsa-miR-4721 6962-6969 -0.275 92.0 -0.002 19.0 noncon
hsa-miR-6823-3p 7022-7028 -0.274 93.0 -0.002 32.0 noncon
hsa-miR-6755-5p 3560-3566 -0.273 90.0 -0.002 21.0 noncon
hsa-miR-3612 548-554 -0.272 95.0 -0.006 29.0 noncon
hsa-miR-6811-5p 261-267 -0.27 94.0 -0.172 91.0 noncon
hsa-miR-4726-5p 1305-1312 -0.27 90.0 -0.006 24.0 noncon
hsa-miR-4532 855-861 -0.269 90.0 -0.006 26.0 noncon
hsa-miR-515-3p 4688-4695 -0.269 96.0 -0.002 35.0 noncon
hsa-miR-4667-5p 3458-3465 -0.268 94.0 -0.002 21.0 noncon
hsa-miR-4433b-3p 6764-6770 -0.267 95.0 -0.002 43.0 noncon
hsa-miR-6824-5p 5655-5661 -0.266 90.0 -0.002 15.0 noncon
hsa-miR-4513 5892-5899 -0.266 95.0 -0.002 33.0 noncon
hsa-miR-6810-5p 474-480 -0.264 95.0 -0.006 41.0 noncon
hsa-miR-5008-5p 1132-1138 -0.263 93.0 -0.006 42.0 noncon
hsa-miR-8089 3458-3465 -0.263 94.0 -0.002 24.0 noncon
hsa-miR-485-3p 322-328 -0.263 96.0 -0.168 93.0 noncon
hsa-miR-4721 6970-6977 -0.262 91.0 -0.002 19.0 noncon
hsa-miR-454-3p 535-541 -0.261 95.0 -0.006 41.0 con
hsa-miR-2114-3p 7022-7028 -0.26 91.0 -0.002 32.0 noncon
hsa-miR-604 1373-1379 -0.26 95.0 -0.006 32.0 noncon
hsa-miR-6751-3p 8208-8215 -0.26 94.0 -0.002 39.0 noncon
hsa-miR-4700-5p 3458-3465 -0.258 94.0 -0.002 22.0 noncon
hsa-miR-5000-3p 102-108 -0.258 95.0 -0.258 96.0 noncon
hsa-miR-130b-3p 535-541 -0.258 94.0 -0.006 43.0 con
hsa-miR-4474-3p 7811-7818 -0.258 93.0 -0.002 26.0 noncon
hsa-miR-7109-3p 7946-7952 -0.257 95.0 -0.002 42.0 noncon
hsa-miR-454-5p 4316-4323 -0.256 92.0 -0.002 29.0 noncon
hsa-miR-5004-5p 4249-4256 -0.255 91.0 -0.002 18.0 noncon
hsa-miR-1199-5p 8208-8215 -0.255 95.0 -0.002 43.0 noncon
hsa-miR-937-5p 144-150 -0.253 96.0 -0.253 97.0 noncon
hsa-miR-2114-5p 972-978 -0.252 95.0 -0.006 35.0 noncon
hsa-miR-4436a 102-108 -0.252 97.0 -0.252 97.0 noncon
hsa-miR-2114-3p 5036-5042 -0.251 91.0 -0.002 32.0 noncon