FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GNT2



General Information
Gene Symbol:   
B3GNT2
Protein name:   
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.149
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• This is the principal enzyme forming polylactosamine [Gal(β1-4)GlcNAc(β1-3)]n structures or "i-antigen" on N- and O-glycans (e.g. core-2 glycans), and glycolipids. It also contributes to chain extension in keratan sulfates.
• Long polylactosamine chains on N-glycans was greatly reduced in the tissues of B3gnt2-/- mice.
• It is structurally similar to β1,3-galactosyltransferases.
• Polylactosamine structures are recognized by LEL/tomato lectin and PWM (pokeweed lectin).
• B3GnT2 and B3GnT8 may form a heterocomplex to facilitate polyLacNAc extension.
• Broad, ubiquitous tissue distribution.
• Lack of the enzyme activity in human results in autoimmune diseases.

Related Pathway
KEGG ORTHOLOGY:   
K00741
KEGG Pathway:   
ko00533  ko00601  ko01100 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9NY97 
NCBI GeneID:   
10678
GlyMap GeneID:   
10678
DNA aRefSeq:   
NM_001319075.1 
Protein RefSeq:   
NP_001306004.1 
BRENDA:   
Kinetic info.
OMIM number:   
605581
GeneCards database:   
B3GNT2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GSL, GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-4)GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.70e-06    (pmol/cell • hr)       KM:    7.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
Transcriptional start site (TSS) 0 200 -2000 -1800 -1600 -1400 -1200 -1000 -800 -600 -400 -200
ENST00000301998 ENST00000405767 RUNX1 KLF9 USF1 USF2 ZNF341 SMAD2 NFE2 ZXDC ZXDC RCOR1 PITX3 ZBTB6 ZBTB6 GLIS1 E2F6 E2F4 KLF9 TFAP4 TEAD4 NFE2 KLF1 NRF1 ZNF18 MYC NFYB AR USF2 KMT2B KLF4 CREM GATA6 ATF1 USF1 SRF STAT5A MITF
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-6502-5p 1073-1080 -0.556 99.0 -0.556 99.0 noncon
hsa-miR-1301-5p 1073-1080 -0.527 99.0 -0.527 99.0 noncon
hsa-miR-4471 45-52 -0.523 99.0 -0.523 99.0 noncon
hsa-miR-1911-5p 148-155 -0.521 98.0 -0.521 99.0 noncon
hsa-miR-4305 478-485 -0.496 98.0 -0.496 99.0 noncon
hsa-miR-8059 45-52 -0.487 99.0 -0.487 99.0 noncon
hsa-miR-4722-3p 203-210 -0.47 99.0 -0.47 99.0 noncon
hsa-miR-6727-3p 203-210 -0.452 99.0 -0.452 99.0 noncon
hsa-miR-5581-5p 803-810 -0.447 98.0 -0.447 98.0 noncon
hsa-miR-4297 803-810 -0.429 98.0 -0.429 98.0 noncon
hsa-miR-4651 545-552 -0.427 95.0 -0.427 96.0 noncon
hsa-miR-325 1165-1172 -0.427 98.0 -0.399 98.0 noncon
hsa-miR-608 545-552 -0.424 95.0 -0.424 96.0 noncon
hsa-miR-548b-3p 1255-1262 -0.414 99.0 -0.387 99.0 noncon
hsa-miR-6864-5p 137-144 -0.397 98.0 -0.397 99.0 noncon
hsa-miR-6128 880-887 -0.384 99.0 -0.384 99.0 noncon
hsa-miR-1299 881-888 -0.378 98.0 -0.378 99.0 noncon
hsa-miR-3187-3p 736-743 -0.378 99.0 -0.378 99.0 noncon
hsa-miR-6798-3p 1154-1160 -0.378 93.0 -0.354 95.0 noncon
hsa-miR-643 920-927 -0.378 98.0 -0.378 99.0 noncon
hsa-miR-6841-5p 257-263 -0.364 96.0 -0.364 97.0 noncon
hsa-miR-6812-5p 546-553 -0.355 96.0 -0.355 97.0 noncon
hsa-miR-3926 289-296 -0.345 98.0 -0.345 98.0 noncon
hsa-miR-195-5p 912-918 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-16-5p 912-918 -0.34 97.0 -0.34 98.0 noncon
hsa-miR-548an 100-107 -0.339 98.0 -0.339 99.0 noncon
hsa-miR-549a 499-506 -0.333 96.0 -0.333 97.0 noncon
hsa-miR-6819-5p 546-553 -0.329 96.0 -0.329 96.0 noncon
hsa-miR-6737-5p 546-553 -0.329 96.0 -0.329 97.0 noncon
hsa-miR-3616-3p 314-320 -0.323 94.0 -0.323 96.0 noncon
hsa-miR-532-5p 805-812 -0.319 97.0 -0.319 98.0 noncon
hsa-miR-8065 331-337 -0.316 94.0 -0.316 95.0 noncon
hsa-miR-4793-5p 206-213 -0.314 98.0 -0.314 98.0 noncon
hsa-miR-642a-5p 161-168 -0.306 98.0 -0.306 98.0 noncon
hsa-miR-4715-5p 739-745 -0.305 95.0 -0.305 97.0 noncon
hsa-miR-7152-3p 797-803 -0.298 95.0 -0.298 96.0 noncon
hsa-miR-1468-3p 17-24 -0.297 98.0 -0.297 99.0 noncon
hsa-miR-5693 1302-1308 -0.296 98.0 -0.278 98.0 noncon
hsa-miR-5003-5p 622-629 -0.292 96.0 -0.292 97.0 noncon
hsa-miR-1468-3p 576-583 -0.286 98.0 -0.286 99.0 noncon
hsa-miR-6866-5p 128-135 -0.285 92.0 -0.285 95.0 noncon
hsa-miR-15b-5p 912-918 -0.285 95.0 -0.285 96.0 noncon
hsa-miR-15a-5p 912-918 -0.285 94.0 -0.285 96.0 noncon
hsa-miR-497-5p 912-918 -0.282 95.0 -0.282 96.0 noncon
hsa-miR-5008-5p 126-132 -0.279 94.0 -0.279 95.0 noncon
hsa-miR-3115 340-346 -0.279 92.0 -0.279 95.0 noncon
hsa-miR-597-5p 600-606 -0.274 94.0 -0.274 96.0 noncon
hsa-miR-6838-5p 912-918 -0.264 93.0 -0.264 95.0 noncon
hsa-miR-6715b-5p 967-973 -0.263 97.0 -0.263 97.0 noncon
hsa-miR-1244 1163-1170 -0.261 94.0 -0.245 95.0 noncon
hsa-miR-141-3p 1049-1055 -0.26 97.0 -0.26 97.0 con
hsa-miR-4733-3p 683-689 -0.259 95.0 -0.259 97.0 noncon
hsa-miR-5684 1168-1174 -0.259 96.0 -0.243 97.0 noncon
hsa-miR-758-5p 766-773 -0.258 98.0 -0.258 98.0 noncon