FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B3GNT7



General Information
Gene Symbol:   
B3GNT7
Protein name:   
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT31
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• This is a Type II Golgi enzyme that aids the biosynthesis of Keratin sulfates (KS).
• KS is a major component of articular cartilage and cornea, while various KS proteins are also found in other tissues and cells.
• B3GNT7 preferentially acts to on sulfated type 2 glycans (Galβ1-4(SO3-6)GlcNAcβ1-3Galβ1-4(SO3-6)GlcNAc).
• It works in concert with CHST6 and B4GalT4 to extend KS polyLacNAc chains, and its function is reduced upon Gal-sulfation by CHST1.
• B3GNT7 is upregulated during ovarian cancer. It results in the expression of the HMOCC-1 mAb epitope.

Related Pathway
KEGG ORTHOLOGY:   
K09664
KEGG Pathway:   
ko00533 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q8NFL0 
NCBI GeneID:   
93010
GlyMap GeneID:   
93010
DNA aRefSeq:   
NM_145236.2 
Protein RefSeq:   
NP_660279.1 
BRENDA:   
Kinetic info.
OMIM number:   
615313
GeneCards database:   
B3GNT7
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), GAG
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     Gal(b1-4)...GlcNAc(b1-?)
Product:  
     GlcNAc(b1-3)Gal(b1-4)...GlcNAc(b1-?)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    3.70e-06    (pmol/cell • hr)       KM:    7.00e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 40 30 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 50 40 30 20 10 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-4665-3p 2210-2217 -0.58 99.0 -0.58 99.0 noncon
hsa-miR-323a-5p 1797-1804 -0.432 98.0 -0.432 98.0 noncon
hsa-miR-6846-5p 2068-2075 -0.403 95.0 -0.403 96.0 noncon
hsa-miR-6848-5p 2068-2075 -0.403 95.0 -0.403 96.0 noncon
hsa-miR-129-1-3p 2148-2155 -0.391 98.0 -0.391 99.0 noncon
hsa-miR-129-2-3p 2148-2155 -0.391 98.0 -0.391 99.0 noncon
hsa-miR-4685-3p 1872-1879 -0.389 99.0 -0.389 99.0 noncon
hsa-miR-4421 1861-1868 -0.377 97.0 -0.377 98.0 noncon
hsa-miR-6867-3p 1873-1880 -0.376 98.0 -0.376 98.0 noncon
hsa-miR-1249-3p 620-627 -0.373 95.0 -0.373 97.0 noncon
hsa-miR-6763-5p 1830-1837 -0.371 97.0 -0.371 98.0 noncon
hsa-miR-4286 1352-1359 -0.368 98.0 -0.368 98.0 noncon
hsa-miR-4287 1872-1879 -0.365 98.0 -0.365 99.0 noncon
hsa-miR-27b-3p 846-853 -0.355 98.0 -0.355 98.0 con
hsa-miR-3144-5p 92-99 -0.355 95.0 -0.355 96.0 noncon
hsa-miR-27a-3p 846-853 -0.355 98.0 -0.355 98.0 con
hsa-miR-181d-3p 1052-1059 -0.344 91.0 -0.344 93.0 noncon
hsa-miR-5699-3p 1861-1868 -0.335 97.0 -0.335 97.0 noncon
hsa-miR-6825-5p 1838-1844 -0.327 91.0 -0.327 93.0 noncon
hsa-miR-3150a-3p 1830-1837 -0.323 96.0 -0.323 97.0 noncon
hsa-miR-939-3p 1755-1762 -0.31 97.0 -0.31 97.0 noncon
hsa-miR-3175 1829-1836 -0.307 95.0 -0.307 96.0 noncon
hsa-miR-6805-5p 988-994 -0.304 90.0 -0.304 92.0 noncon
hsa-miR-5681a 2110-2117 -0.295 97.0 -0.295 98.0 noncon
hsa-miR-4726-3p 1381-1388 -0.286 98.0 -0.286 98.0 noncon
hsa-miR-1178-5p 2107-2113 -0.285 94.0 -0.285 96.0 noncon
hsa-miR-615-5p 2121-2127 -0.283 92.0 -0.283 94.0 noncon
hsa-miR-5588-3p 1165-1171 -0.283 95.0 -0.283 97.0 noncon
hsa-miR-6798-5p 1976-1982 -0.281 94.0 -0.281 96.0 noncon
hsa-miR-433-5p 2031-2037 -0.28 90.0 -0.28 94.0 noncon
hsa-miR-1254 1568-1575 -0.279 97.0 -0.279 98.0 noncon
hsa-miR-4697-5p 2069-2075 -0.273 90.0 -0.273 92.0 noncon
hsa-miR-6862-3p 1517-1524 -0.272 94.0 -0.272 96.0 noncon
hsa-miR-342-3p 2020-2027 -0.271 97.0 -0.271 98.0 noncon
hsa-miR-3936 2123-2130 -0.271 95.0 -0.271 96.0 noncon
hsa-miR-874-5p 1239-1246 -0.263 90.0 -0.263 92.0 noncon
hsa-miR-6837-5p 820-827 -0.262 93.0 -0.262 95.0 noncon
hsa-miR-3116 1568-1575 -0.261 97.0 -0.261 98.0 noncon
hsa-miR-6784-3p 1517-1524 -0.259 93.0 -0.259 95.0 noncon
hsa-miR-4685-5p 820-827 -0.257 93.0 -0.257 95.0 noncon
hsa-miR-6506-5p 2008-2015 -0.255 98.0 -0.255 98.0 noncon
hsa-miR-200a-5p 1906-1913 -0.253 97.0 -0.253 98.0 noncon
hsa-miR-6852-5p 1660-1667 -0.252 95.0 -0.252 96.0 noncon
hsa-miR-1343-3p 1756-1763 -0.251 94.0 -0.251 96.0 noncon
hsa-miR-6507-3p 1167-1173 -0.25 96.0 -0.25 98.0 noncon