FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
B4GALT2



General Information
Gene Symbol:   
B4GALT2
Protein name:   
Beta-1,4-galactosyltransferase 2
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
GT7
E.C. No:   
2.4.1.*
Organs:   
Reaction (SNFG):   
+
→
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• In enzymology assays, β4GalT1 and β4Gal2 display similar specificity for Type II LacNAc chains. Both enzymes preferring the branch of GlcNAcβ1-2Manα1-3 and GlcNAcβ1-4Manα1-3 arm over GlcNAcβ1-2Manα1-6. But, both can transfer β4-Gal residues to N-glycans in the tetra antennary glycan.

Related Pathway
KEGG ORTHOLOGY:   
K07967
KEGG Pathway:   
ko00052  ko00510  ko00513  ko00514  ko00515  ko00533  ko00601  ko01100 
Reactome:   
R-HSA-2022854
Related Database
Organism:   
Homo sapiens
Uniprot:   
O60909 
NCBI GeneID:   
8704
GlyMap GeneID:   
8704
DNA aRefSeq:   
NM_001005417.2 
Protein RefSeq:   
NP_001005417.1 
BRENDA:   
Kinetic info.
OMIM number:   
604013
GeneCards database:   
B4GALT2
Enzyme Specificity
Glycan Type:  
     N-linked, O-linked (mucin type), O-linked (other), GSL
Compartment:  
     Cis, Medial, Trans, TGN
Substrate:  
     GlcNAc(b1-?)
Product:  
     Gal(b1-4)GlcNAc(b1-?)
Constraints:  
     !Gal(b1-3)GlcNAc(b1-?)&!@GlcNAc(b1-4)...Man(b1-4)
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:    5.75e-04    (pmol/cell • hr)       KM:    1.50e+08    (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 20 10 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 180 160 140 120 100 80 60 40 20 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1181 926-933 -0.891 97.0 -0.003 8.0 noncon
hsa-miR-1538 832-839 -0.694 99.0 -0.003 12.0 noncon
hsa-miR-4745-3p 832-839 -0.689 99.0 -0.003 12.0 noncon
hsa-miR-4449 913-920 -0.613 98.0 -0.003 11.0 noncon
hsa-miR-885-3p 855-862 -0.592 99.0 -0.002 28.0 noncon
hsa-miR-4313 479-486 -0.591 99.0 -0.591 99.0 noncon
hsa-miR-1281 923-930 -0.578 99.0 -0.002 18.0 noncon
hsa-miR-4313 389-396 -0.549 98.0 -0.549 99.0 noncon
hsa-miR-6805-5p 131-138 -0.498 98.0 -0.498 99.0 noncon
hsa-miR-6794-5p 240-247 -0.456 98.0 -0.456 98.0 noncon
hsa-miR-6068 293-300 -0.44 95.0 -0.44 97.0 noncon
hsa-miR-6778-3p 581-588 -0.431 99.0 -0.431 99.0 noncon
hsa-miR-4726-5p 809-816 -0.398 96.0 -0.002 17.0 noncon
hsa-miR-4716-3p 240-247 -0.397 97.0 -0.397 98.0 noncon
hsa-miR-4640-5p 809-816 -0.38 96.0 -0.002 18.0 noncon
hsa-miR-3147 812-819 -0.374 98.0 -0.002 35.0 noncon
hsa-miR-6871-3p 693-699 -0.364 98.0 -0.364 98.0 noncon
hsa-miR-4753-5p 879-886 -0.354 98.0 -0.002 21.0 noncon
hsa-miR-185-3p 514-521 -0.353 93.0 -0.353 95.0 noncon
hsa-miR-185-3p 251-258 -0.348 93.0 -0.348 94.0 noncon
hsa-miR-6886-3p 791-798 -0.341 95.0 -0.002 29.0 noncon
hsa-miR-3151-3p 599-605 -0.328 95.0 -0.328 97.0 noncon
hsa-miR-34a-5p 287-293 -0.314 92.0 -0.314 94.0 con
hsa-miR-7152-3p 527-534 -0.313 95.0 -0.313 97.0 noncon
hsa-miR-3194-5p 61-68 -0.31 96.0 -0.31 97.0 noncon
hsa-miR-6791-3p 637-644 -0.309 97.0 -0.309 98.0 noncon
hsa-miR-1910-5p 90-97 -0.309 96.0 -0.309 97.0 noncon
hsa-miR-4264 92-99 -0.307 97.0 -0.307 98.0 noncon
hsa-miR-6887-3p 449-456 -0.304 91.0 -0.304 93.0 noncon
hsa-miR-6852-5p 517-524 -0.291 97.0 -0.291 97.0 noncon
hsa-miR-6826-3p 450-457 -0.287 93.0 -0.287 95.0 noncon
hsa-miR-331-3p 444-451 -0.282 97.0 -0.282 97.0 con
hsa-miR-5189-3p 566-572 -0.279 93.0 -0.279 96.0 noncon
hsa-miR-371b-3p 794-800 -0.278 93.0 -0.001 26.0 noncon
hsa-miR-6829-3p 637-644 -0.277 97.0 -0.277 98.0 noncon
hsa-miR-6868-5p 37-44 -0.272 95.0 -0.272 96.0 noncon
hsa-miR-6835-3p 695-702 -0.268 98.0 -0.268 99.0 con
hsa-miR-7704 232-238 -0.267 93.0 -0.267 94.0 noncon
hsa-miR-6829-5p 513-519 -0.267 93.0 -0.267 95.0 noncon
hsa-miR-1910-5p 44-51 -0.267 94.0 -0.267 96.0 noncon
hsa-miR-19b-3p 675-681 -0.261 91.0 -0.261 93.0 con
hsa-miR-19a-3p 675-681 -0.261 91.0 -0.261 93.0 con
hsa-miR-6769b-3p 633-640 -0.251 95.0 -0.251 96.0 noncon