FuncClass

GlycoEnzDB
Comprehensive glycoEnzyme database and dynamic pathway generation

Description: The GlycoEnzDB is a manually curated glycoEnzyme database, primarily focused on humans. It covers 390 enzymes across 28 pathway maps. Facilities are also available to create custom glycosylation reaction pathways using experimental data in SBML format and for pathway simulation
Contacts: Sriram Neelamegham (neel@buffalo.edu), Yusen Zhou (yusenzho@buffalo.edu) or Ted Groth (tgroth@buffalo.edu).

GlycoEnzDB
Create glycosylation pathway
Custom database generation
Instructions/Reference
 
Pathway Map
DPAGT1



General Information
Gene Symbol:   
DPAGT1
Protein name:   
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Enzyme Class:   
Glycosyltransferase
CAZy Family:   
E.C. No:   
2.7.8.15
Organs:   
Reaction (SNFG):   
+ Dol-P →
+ UDP
Reaction (IUBMB):   
No specified reaction.
About

• ALG7/DPAGT1 initiates the synthesis of 14 sugar Glc3Man9GlcNAc2 on dolichol pyrophosphate. This is a lipid linked oligosaccaride (LLO) where 7 monosaccharides are added in the cytoplasmic side and 7 in the ER lumen.
• ALG7 adds GlcNAc to dolichol phosphate (P-Dol) to generate GlcNAc-PP-Dol.
• Tunicamycin acts as a tight-binding competitive inhibitor of DPAGT1, presumably because it structurally resembles the donor nucleotide sugar.
• Mice lacking DPAGT1 are embyonic lethal in early days following implantation.
• CDG type I are a class of Congenital Disorders of Glycosylation that reduces site occupany of N-glycans. Enzymes causing CDG-I are commonly associated with deficiencies in LLO biosynthesis.
• ALG7/DPAGT1 Mutations in human results in CDG-Ij. It also causes Limb-Girdle congential myasthenic syndrome.

Related Pathway
KEGG ORTHOLOGY:   
K01001
KEGG Pathway:   
No Record 
Reactome:   
R-HSA-446193
Related Database
Organism:   
Homo sapiens
Uniprot:   
Q9H3H5 
NCBI GeneID:   
1798
GlyMap GeneID:   
1798
DNA aRefSeq:   
NM_001382.3 
Protein RefSeq:   
NP_001373.2 
BRENDA:   
Kinetic info.
OMIM number:   
191350
GeneCards database:   
DPAGT1
Enzyme Specificity
Glycan Type:  
     N-linked
Compartment:  
     ER
Substrate:  
     Dol-P
Product:  
     GlcNAc(a1-?)Dol-P-P
Simulation Parameters
Mechaelis-Menten parameters (suggested)
VM:       (pmol/cell • hr)       KM:       (pM)
Relative conc of enzymes in compartment.(sum will be normalized to 1)
ER Cis- Medial- Trans- TGN










Expression Data in Tissue {ref. 5} Source from NCBI gene database
adrenal appendix bone marrow brain colon duodenum endometrium esophagus fat gall bladder heart kidney liver lung lymph node ovary pancreas placenta prostate salivary gland skin small intestine spleen stomach testis thyroid urinary bladder 12 10 8 6 4 2 0 RPKM
Expression Data in Cells {ref. 13} Source from Cancer Cell Line Encyclopedia database
Kidney Lung Breast Prostate Large intestine Ovary HL60 haematopoietic and lymphoid tissue Skin Central nervous system 786O A498 ACHN CAKI1 UO31 A549 EKVX HOP62 HOP92 NCIH226 NCIH23 NCIH322 NCIH460 NCIH522 SHP77 BT549 HS578T MCF7 MDAMB231 MDAMB468 T47D DU145 PC3 HCT116 HCT15 HT29 KM12 SW620 OVCAR4 OVCAR8 IGROV1 SKOV3 HL60 K562 RPMI8226 LOXIMVI MALME3M RPMI7951 SKMEL28 SKMEL5 UACC257 UACC62 SF268 SF295 SF539 SNB75 400 300 200 100 0 Transcripts per million (TPM)
Transcription factor {ref. 10}
No high degree of confidence data
miRNA-GlycoEnzyme Interactome {ref. 9}
miRNA Name Pos. Context Score Frac. Wt. Context Frac. Conserved Quality
hsa-miR-1294 22-29 -0.661 99.0 -0.661 99.0 noncon
hsa-miR-765 112-119 -0.561 99.0 -0.561 99.0 noncon
hsa-miR-663b 442-449 -0.479 99.0 -0.479 99.0 noncon
hsa-miR-574-5p 299-306 -0.457 98.0 -0.457 98.0 noncon
hsa-miR-376b-5p 295-302 -0.456 98.0 -0.456 99.0 noncon
hsa-miR-3136-3p 356-363 -0.442 99.0 -0.442 99.0 noncon
hsa-miR-485-5p 256-263 -0.436 99.0 -0.436 99.0 noncon
hsa-miR-7155-3p 356-363 -0.434 99.0 -0.434 99.0 noncon
hsa-miR-6884-5p 256-263 -0.42 99.0 -0.42 99.0 noncon
hsa-miR-5701 463-470 -0.4 98.0 -0.399 99.0 noncon
hsa-miR-5702 43-50 -0.399 98.0 -0.399 98.0 noncon
hsa-miR-199a-3p 188-195 -0.393 98.0 -0.393 98.0 noncon
hsa-miR-199b-3p 188-195 -0.393 98.0 -0.393 98.0 noncon
hsa-miR-376c-5p 295-302 -0.375 98.0 -0.375 98.0 noncon
hsa-miR-4733-5p 344-351 -0.373 99.0 -0.373 99.0 noncon
hsa-miR-136-5p 457-464 -0.364 98.0 -0.363 98.0 noncon
hsa-miR-589-3p 415-422 -0.352 99.0 -0.352 99.0 noncon
hsa-miR-6779-3p 362-369 -0.34 98.0 -0.34 99.0 noncon
hsa-miR-3129-5p 188-195 -0.331 98.0 -0.331 98.0 noncon
hsa-miR-516b-5p 115-121 -0.327 97.0 -0.327 97.0 noncon
hsa-miR-6731-5p 232-238 -0.32 96.0 -0.32 97.0 noncon
hsa-miR-5584-3p 428-435 -0.319 98.0 -0.319 99.0 noncon
hsa-miR-4419a 234-240 -0.316 96.0 -0.316 97.0 noncon
hsa-miR-8085 232-238 -0.309 95.0 -0.309 96.0 noncon
hsa-miR-6129 234-240 -0.305 95.0 -0.305 96.0 noncon
hsa-miR-6130 234-240 -0.305 95.0 -0.305 96.0 noncon
hsa-miR-4510 234-240 -0.305 95.0 -0.305 96.0 noncon
hsa-miR-6811-3p 374-380 -0.296 96.0 -0.296 97.0 noncon
hsa-miR-6127 234-240 -0.294 94.0 -0.294 95.0 noncon
hsa-miR-6133 234-240 -0.294 94.0 -0.294 95.0 noncon
hsa-miR-4677-3p 24-30 -0.289 97.0 -0.289 98.0 noncon
hsa-miR-3911 297-303 -0.288 95.0 -0.288 97.0 noncon
hsa-miR-6873-5p 235-241 -0.287 96.0 -0.287 97.0 noncon
hsa-miR-4695-5p 79-85 -0.285 96.0 -0.285 97.0 noncon
hsa-miR-6728-5p 243-249 -0.279 96.0 -0.279 97.0 noncon
hsa-miR-6810-3p 437-443 -0.275 93.0 -0.275 95.0 noncon
hsa-miR-5000-3p 316-322 -0.271 95.0 -0.271 96.0 noncon
hsa-miR-873-3p 28-34 -0.27 97.0 -0.27 98.0 noncon
hsa-miR-6809-5p 83-89 -0.268 93.0 -0.268 95.0 noncon
hsa-miR-202-3p 21-27 -0.265 93.0 -0.265 95.0 noncon
hsa-miR-185-5p 231-237 -0.258 94.0 -0.258 96.0 noncon
hsa-miR-1827 77-83 -0.254 93.0 -0.254 95.0 noncon
hsa-miR-6801-3p 437-443 -0.254 91.0 -0.254 93.0 noncon
hsa-miR-4728-3p 130-136 -0.252 96.0 -0.252 97.0 noncon
hsa-miR-8082 240-246 -0.251 93.0 -0.251 94.0 noncon